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WarmStart® Colorimetric LAMP 2X Master Mix with UDG
Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets
Fast, clear, pink-to-yellow visible detection of amplification
Reduce risk of carryover contamination with thermolabile UDG and dUTP included in the master mix
WarmStart® feature inhibits enzyme activity at room temperature allowing for flexible reaction setup
Learn more about LAMP and other isothermal amplification methods
Learn about a colorimetric RT-LAMP assay used for the detection of Eastern equine encephalitis virus in mosquitoes: AMCA publication
Read how NEB developed and implemented a SARS-CoV-2 RT-LAMP workflow for workplace surveillance: 1) NEB Expressions article 2) PLOS ONE publication
A publication by members of the global LAMP (gLAMP) Consortium provides a comprehensive review of LAMP and its role in the COVID-19 pandemic
Negative reactions are indicated in pink and positive reactions are indicated by a change to yellow.
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NEB® LAMP Primer Design Tool Tutorial
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产品信息
WarmStart Colorimetric LAMP 2X Master Mix with UDG is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of LAMP and RT-LAMP reactions. The inclusion of dUTP and UDG in the master mix reduces the possibility of carryover contamination between reactions.
This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow (an overview of LAMP and primer design can be found in the Featured Videos section). This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15–40 minutes.
Simple Visualization of Amplification Success
Negative reactions are indicated in pink and positive reactions are indicated by a change to yellow.
What is the difference between using Colorimetric LAMP and regular LAMP reactions?
How long should I incubate the Colorimetric LAMP reaction before checking the results?
What buffer should I use for my target samples and primers going into the colorimetric LAMP reaction?
What is the Mg++ concentration in the Colorimetric LAMP 2X Master Mix?
Can the Colorimetric LAMP 2X Master Mix be used in instruments that utilize fluorescent detection of DNA amplification or real time turbidity measurement?
How stable are the WarmStart Colorimetric LAMP 2X Master Mixes?
How do I design LAMP Primers?
The WarmStart LAMP Master Mix has some precipitation in the tube after thawing, is this normal?
What is the difference between the WarmStart® Colorimetric LAMP 2X Master Mix with UDG (NEB #M1804) and the WarmStart Colorimetric LAMP 2X Master Mix (DNA & RNA) (NEB #M1800)?
What if my colorimetric LAMP mix is orange instead of pink prior to amplification?
Does the WarmStart® Colorimetric LAMP Master Mix with UDG enable carryover prevention/contamination reduction?
How much sample can be added to the colorimetric LAMP reaction as input?
Can I monitor the color change of the colorimetric LAMP reactions using a spectrophotometer?
What components are contained in the WarmStart® Colorimetric LAMP Master Mix with UDG and what else I need to perform a LAMP reaction?
Can the WarmStart Colorimetric LAMP 2X Master Mixes be used in instruments that utilize fluorescence detection?
What is the optimal LAMP/RT-LAMP amplification temperature?
How fast should I expect a result?
Can I set up my LAMP/RT-LAMP reactions at room temperature?
Amplification occurred in my NTC sample(s) following isothermal incubation. What happened?
Is a separate incubation step required for RT-LAMP?
Should I use Guanidine HCl (B2619A) in colorimetric LAMP reactions?
Does NEB have a master mix for LAMP or RT-LAMP reactions?
What is LAMP and RT-LAMP?
引用 & 技术文献
专题文章
Many Initiatives Turning to RT-LAMP as Alternative to PCR for Rapid COVID-19 Screening Assays
Mitigating Risk and Ensuring Consistent Supply Chain Through Internal SARS-CoV-2 Testing with RT-LAMP
