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Thermolabile Exonuclease I
Interested in a 5-minute protocol? Try out the Exo-CIP™ Rapid PCR Cleanup Kit (NEB #E1050)
We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.
- DNA specific exonuclease
- Catalyzes the removal of nucleotides from linear single-stranded DNA in the 3′ to 5′ direction
- Unlike Exonuclease I (NEB #M0293), Thermolabile Exonuclease I can be heat inactivated at 80°C in 1 minute
Thermolabile Exonuclease I is ideal for:
- Removal of single-stranded primers in PCR reactions prior to DNA sequencing or SNP analysis
- Removal of single-stranded primers for nested PCR reactions
- Removal of linear single-stranded DNA, leaving behind double-stranded DNA in the sample
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What are exonucleases and their applications?
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