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Lambda Exonuclease
- DNA specific exonuclease
- Catalyzes the removal of nucleotides from linear or nicked double-stranded DNA in the 5′ to 3′ direction
- Highly processive degradation of double-stranded DNA from the 5′ end
- Preferred substrate is 5′-phosphorylated double-stranded DNA although non-phosphorylated substrates are degraded at a greatly reduced rate
Lambda Exonuclease is ideal for:
- Conversion of linear double-stranded DNA to single-stranded DNA via preferred activity on 5′-phosphorylated ends
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