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Mismatch Endonuclease I
- DNA Endonuclease
- Catalyzes the cleavage of some DNA mismatches (T:T, G:G and G:T)
- Cleaves the 3rd phosphodiester bond on the 5′ side of the mismatched base in both strands leaving a 5 bp overhang with a 3′- OH and 5′- phosphate
- Best at T mismatches; does not recognize all DNA mismatches
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