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T4 DNA Ligase
Looking for T4 DNA Ligase alternatives? Try Salt-T4 or Hi-T4 DNA Ligases
T4 DNA Ligase will ligate these substrates:
dsDNA
Nicked DNA/RNA
- Catalyzes the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA.
- This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids (1).
- The industry standard for performance and quality
- Applicable for use in high-complexity library cloning
- Other T4 DNA Ligase products include Quick Ligation Kit, Salt-T4, Hi-T4, Instant Sticky-end Ligase Master Mix and Blunt/TA Ligase Master Mix
- This product can be purchased in larger volumes. Submit an inquiry to find out more.
- Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart or DNA Ligase Selection Chart
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Quick Tips – Do I need a 5′ phosphate for ligation?
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Need a custom/large volume order? Contact Us
Bulk packaging may also be available and requested for large recurring orders.
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