NEBridge^® Ligase Master Mix

Offering flexibility and convenience for users, NEBridge Ligase Master Mix performs high efficiency and high-fidelity Golden Gate Assembly with a broad assortment of NEB Type IIS restriction enzymes.

• Optimized for efficient and accurate Golden Gate Assembly
• Convenient 3X Master Mix format
• Use with NEB Type IIS restriction enzymes
• Use for seamless cloning – no scar remains following assembly
• Ideal for ordered assembly of multiple fragments (2-25+) in a single reaction
• Can also be used for cloning of single inserts and library construction
• Free tool available at GoldenGate.neb.com
• Try our free Ligase Fidelity Tools ( for the design of high-fidelity Golden Gate Assemblies)