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NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs Set 2)
For optimal workflow flexibility, NEBNext® Multiplex Oligos are available separately from library preparation kits, providing adaptors and primers for high-yield multiplex Illumina® library production. The NEBNext Adaptor has a unique hairpin loop structure that minimizes adaptor-dimer formation, while NEBNext index PCR primers enable index (barcode) incorporation during library amplification. This second set of unique-dual indices (UDIs) includes 96 pre-mixed unique pairs of index primers (i5 and i7), packaged in a single-use 96-well plate with a pierceable foil seal.
For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.
- Increased sample identification specificity
- Enables complete filtering of index-hopped reads
- Highly efficient adaptor ligation
- Minimized adaptor-dimer formation
- Includes index primers for library multiplexing
- Combine with sets 1, 3, and 4 for 384 indices