NEBNext^® Ultra^™ II RNA First Strand Synthesis Module

This module is part of the Ultra II Directional RNA and Ultra II RNA workflows, for Illumina^®– compatible RNA library construction. These workflows are compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enable high yield preparation of high quality libraries from 5 ng – 1 µg total RNA.

Kits are also available that include reagents for every step in the Ultra II Directional RNA library construction workflow (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760) and NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads (NEB #E7765)) and non-directional Ultra II RNA library construction workflow (NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770) and NEBNext Ultra II RNA Library Prep with Sample Purification Beads (NEB #E7775)).