NEBNext^® Multiplex Oligos for Illumina^® (96 Unique Dual Index Primer Pairs Set 3)

For optimal workflow flexibility, NEBNext^® Multiplex Oligos are available separately from library preparation kits, providing adaptors and primers for high-yield multiplex Illumina^® library production. The NEBNext Adaptor has a unique hairpin loop structure that minimizes adaptor-dimer formation, while NEBNext index PCR primers enable index (barcode) incorporation during library amplification. This third set of unique-dual indices (UDIs) includes 96 pre-mixed unique pairs of index primers (i5 and i7), packaged in a single-use 96-well plate with a pierceable foil seal.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.

• Enables complete filtering of index-hopped reads
• Highly efficient adaptor ligation
• Minimized adaptor-dimer formation
• Includes index primers for library multiplexing
• Combine with sets 1, 2, and 4 for 384 barcodes