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E. coli Poly(A) Polymerase
Poly(A) Polymerase catalyzes the template independent addition of AMP from ATP to the 3´ end of RNA
- Isolated from a recombinant source
- Applications include 3′ labeling of RNA with ATP or Cordycepin and poly(A) tailing of RNA for cloning or affinity purification
- Enhances translation of RNA transferred into eukaryotic cells
- Tested for the absence of DNases and RNases