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NEBridge® Golden Gate Assembly Kit (BsaI-HF® v2)
Learn about Ligase Fidelity and Push the Limits of Golden Gate Assembly (50+ fragments)
- Updated to include BsaI-HFv2 (optimized for Golden Gate)
- Seamless cloning – no scar remains following assembly
- Includes destination plasmid with T7/SP6 promoters
- Ordered assembly of multiple fragments (2-50+) in a single reaction
- Can also be used for cloning of single inserts and library preparations
- Efficient with regions with high GC content and areas of repeats
- Compatible with a broad range of fragment sizes (< 100 bp to > 15 kb)
- Free tool available at GoldenGate.neb.com
- Try our Ligase FidelityTools (for the design of high-fidelity Golden Gate Assemblies)
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Golden Gate Assembly Workflow
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