NEB^® PCR Cloning Kit (Without Competent Cells)

Easy cloning of all PCR products, including blunt and TA ends

• Updated to allow for in vitro transcription with both SP6 and T7 promoters
• BsaI-site removed from the ampicillin-resistant gene (allows for the cloning of Golden Gate Assembly modules)
• Fast cloning experiments with 5-minute ligation step
• Simplified screening with low/no colony background and no blue/white selection required
• Save time by eliminating purification steps
• Flanking restriction sites available for easy subcloning, including choice of two single digest options
• Provided analysis primers allow for downstream colony PCR screening or sequencing
• Ready-to-use kit components include 1 kb control amplicon, linearized cloning vector and single-use competent E. coli
• Longer shelf life (12 months), as compared to some commercially available products
• Also available with competent cells 

说明书