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T7 Endonuclease I
EnGen Mutation Detection Kit (NEB #E3321 ) Now Available
- DNA Endonuclease
- Catalyzes the cleavage of DNA mismatches and non-β DNA structures including Holliday junctions and cruciform leaving 3′-OH and 5′-phosphate
- Best at C mismatches and does not recognize all DNA mismatches
- To a lesser extent cleaves across a nick in dsDNA
- Key enzyme for use in genome editing mutation detection workflows
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What are endonucleases and their applications?
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