EnGen Lba Cas12a (Cpf1) |NEB酶试剂 New England Biolabs

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EnGen® Lba Cas12a (Cpf1)

EnGen Lba Cas12a (Cpf1) is a programmable DNA endonuclease guided by a single guide RNA (gRNA). Targeting requires a gRNA complementary to the target site as well as a 5′ TTTV protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. Cleavage by EnGen Lba Cas12a (Cpf1) occurs ~17 bases 3′ of the PAM and leaves 5′ overhanging ends.

  • T-rich TTTV PAM sequence opens up additional genomic regions for targeting
  • Shorter, 40-44 base guide RNA
  • Two nuclear localization signals for improved transport to the nucleus
  • 5’ overhanging termini on cleavage products
  • Active from 16 to 48°C
  • Maintains activity at lower temperatures than the Acidaminococcus orthologs, permitting editing in ectothermic organisms such as zebra fish and xenopus
  • High concentration liquid format can be used for microinjection, electroporation and lipofection.

EnGen Lba Cas12a (Cpf1) |

库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M0653S 1 µM 70 pmol ¥839.00 有 有 有 有 有
M0653T 100 µM 2,000 pmol ¥2,999.00 有 有 有 有 有
如何订购
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库存 货号 浓度 规格 目录价 北京 上海 广州 成都 苏州 M0653S 1 µM 70 pmol ¥839.00 有 有 有 有 有 M0653T 100 µM 2,000 pmol ¥2,999.00 有 有 有 有 有