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EpiMark® 5-hmC and 5-mC Analysis Kit
The EpiMark® 5-hmC and 5-mC Analysis Kit can be used to analyze and quantitate 5-methylcytosine and 5-hydroxymethylcytosine within a specific locus. Complete conversion of 5-hmC to glucosylated 5-hmC in DNA by T4 β-glucosyltransferase (T4-BGT).
- Discrimination between 5-mC and 5-hmC in CCGG sequences using enzymatic digestion and PCR amplification
- Relative quantitation of 5-mC and 5-hmC
- Easy-to-use protocol
- This kit contains enough material for 20 reactions