NEBNext^® Enzymatic Methyl-seq Kit

NEBNext Enzymatic Methyl-seq (EM-seq™) is a new method for identification of 5-mC and 5-hmC.

While bisulfite sequencing has been the gold standard for methylome analysis, this conversion treatment damages DNA, resulting in fragmentation, loss and bias. 

In contrast, the highly effective enzyme-based conversion in the NEBNext Enzymatic Methyl-seq Kit minimizes damage to DNA and, with the supplied NEBNext Ultra™ II library preparation workflow reagents, produces high quality libraries that enable superior detection of 5-mC and 5-hmC from fewer sequencing reads.

• Superior sensitivity of detection of 5-mC and 5-hmC 
• Greater mapping efficiency
• More uniform GC coverage 
• Detection of more CpGs with fewer sequence reads
• Uniform dinucleotide distribution
• High-efficiency library preparation, with larger library insert sizes
• Conversion module also available separately

View or download extensive performance data in our Technical Note.