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Anti-MBP Magnetic Beads
An affinity matrix for small-scale isolation and purification of (MBP) fusion proteins.
- Monoclonal anti-MBP is covalently coupled to 1 µm nonporous superparamagnetic particles through a linkage that is stable and leak resistant over a wide pH range, thereby permitting the immunomagnetic isolation of MBP-fusion proteins from cell culture extracts
- Immobilized fusion proteins can be used in subsequent experiments to capture (pull down) target proteins that specifically interact with the immobilized MBP fusion protein
- Binding capacity of 5 μg MBP5*-paramyosin ΔSal fusion protein/mg Anti-MBP Magnetic Beads
- Quick, small-scale purification of MBP-fusion proteins enables higher throughput and efficiency
