NEBExpress^® Ni-NTA Magnetic Beads

• An affinity matrix for the small-scale isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins in manual or automated formats.
• Prepared with agarose based, super-paramagnetic microparticles which provide high binding capacity and fast magnetic response permitting high throughput and scalable purification strategies of His tagged-fusion proteins.
• NTA coordination exhibits low Nickel ion leaching.
• Tolerates a wide range of conditions, including the presence of protein denaturants and detergents. Compatible with commercially available detergent based cell lysis reagents.
• Elution of the target protein may be achieved by protonation, ligand exchange (with imidazole) or extraction of the metal ion by a stronger chelator (e.g. EDTA).
• Extremely low non-specific binding properties permit immobilized fusion proteins to be used in subsequent experiments to capture or pull down interacting proteins from crude cell lysates