NEBExpress^® E. coli Lysis Reagent 

NEBExpress E. coli Lysis Reagent is a chemical lysis solution composed of a proprietary mix of non-ionic and zwitterionic detergents and Tris-based buffer. It allows disruption of E. coli cells without denaturing soluble proteins.

• Ready-to-use liquid that is stable at room temperature
• Gentle yet highly active formulation of detergents in Tris buffer
• Scalable lysis reactions from small to large bacterial cell pellets and compatible with high throughput workflows 
• Suitable for extracting proteins, especially thermosensitive proteins vulnerable to mechanical lysis procedures
• Compatible with analyses such as SDS-PAGE, Western blots, activity assay, immunoprecipitation, and downstream purification 
• Suitable for disrupting most Gram-negative bacterial cells