The TEV protease recognition sequence with the highest catalytic efficiency is ENLYFQ ▼S; however, the amino acid in the P1’ position can also be G, A, M, C, or H (1).

• Removal of affinity purification tags such as maltose-binding protein (MBP) or poly-histidine from fusion proteins
• Optimal activity and stability for up to 24 months
• Active in a wide range of buffers; optimal activity between pH 6.0 and 9.0.
• High substrate specificity with no non-specific proteolysis
• TEV Protease contains a 7x His-Tag for easy removal from a reaction using NEBExpress^® Ni Resin (NEB# S1428S), NEBExpress^® Ni Spin Columns (NEB# S1427S/L), or NEBExpress^® Ni-NTA Magnetic Beads (NEB# S1423S)