Deglycosylation in minutes for N-glycan analysis

Rapid PNGase F enables complete and rapid deglycosylation of antibodies and immunoglobulin fusion proteins, as well as other glycoproteins, in minutes. All N-glycans are released rapidly and without bias, and are ready to be prepared for downstream chromatography or mass spectrometry analysis. Rapid PNGase F creates an optimized workflow, reducing processing time without compromising sensitivity or reproducibility.

• Complete deglycosylation of antibodies and immunoglobulin fusion proteins in 10 minutes
• Recombinant enzyme; guaranteed endoglycosidase F1, F2 or F3 free
• All reaction components are compatible with HPLC and mass spectrometry analysis
• ≥ 95% purity, as determined by SDS-PAGE and intact ESI-MS
• Optimal activity and stability ensured for up to 12 months
• Fast reaction setup

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One-step protocol for deglycosylation with Rapid PNGase F