| 产品编号 | 产品名称 | 产品规格 | 产品等级 | 产品价格 |
| 631-10389 | （AKRLP-011）Lbis® Mouse Leptin ELISA kit Lbis®小鼠瘦素 ELISA试剂盒 |
96 tests | – | – |
- 产品特性
- 相关资料
- Q&A
- 参考文献
Lbis® Leptin-Mouse
Shibayagi 小鼠瘦素 ELISA试剂盒
瘦素（Leptin）是一种分子量为16KD的蛋白质类激素。主要由白色脂肪组织产生，其它组织如褐色脂肪组织、胎盘、卵巢、骨骼肌、胃底部、乳腺上皮细胞、骨髓、下垂体、肝脏等也能检测到。其在大鼠和小鼠中有97％的同源性。瘦素在突变种肥胖小鼠中发现，是通过调节食欲和新陈代谢来参与至能量平衡的一种重要的激素。血液中瘦素的浓度会因为空腹和低热量饮食而降低，但进食后并不会有明显增加，只有肥胖即脂肪组织量增大的情况下才会增加，可反映身体脂肪量。瘦素进入大脑，通过抑制体内下丘脑神经肽Y（NPY）和刺鼠基因相关蛋白(AgRP)表达神经的受体，另外活化体内α-MSH表达神经，起到降低食欲，减少进食的作用。但是，对于严重肥胖的人，虽然他们血液中瘦素的浓度很高，但对瘦素有抗性，瘦素并不能发挥抑制食欲的作用。全身性脂肪萎缩病引起的瘦素降低或缺乏，会造成胰岛素抗性、糖尿病、脂肪肝、高甘油三酯血症等疾病。除此之外，瘦素也被认为与对动脉粥样硬化的免疫反应、能量平衡和性周期相关，与肺泡表面活性剂的产生也有关系。
◆特点
• 短时间测定（总的反应时间：3小时）
• 微量样品（标准操作：10 μL）可测
• 使用对环境无害的防腐剂
• 全部试剂均为液体，可直接使用
• 精密的测定精度和高再现性
◆构成
|
组成 |
状态 |
容量 |
|
(A)抗体固相化 96 孔板（干燥孔板） |
洗净后使用 |
96 wells（8×12）／1 块 |
|
(B)瘦素标准溶液（小鼠）（5,000 pg/ml） |
稀释后使用 |
500 μL／1 瓶 |
|
(C)缓冲液 |
即用 |
60 mL／1 瓶 |
|
(D)生物素结合抗瘦素抗体 |
稀释后使用 |
200 μL／1 瓶 |
|
(E)过氧化物・抗生物素蛋白结合物 |
稀释后使用 |
200 μL／1 瓶 |
|
(F)显色液（TMB） |
即用 |
12 mL／1 瓶 |
|
(H)反应终止液（1M H2SO4）※小心轻放 |
即用 |
12 mL／1 瓶 |
|
( I )浓缩洗净液（10×） |
稀释后使用 |
100 mL／1 瓶 |
|
封板膜 |
3 张 |
|
|
使用说明书 |
1 份 |
◆交叉反应
※交叉反应是浓度为3,000 pg/mL时的数据
|
动物种类 |
对象物质 |
反应性和反应率（％） |
|
Mouse |
Leptin |
100 |
|
α-MSH |
― |
|
|
IFN-γ |
― |
|
|
MCH |
― |
|
|
TNF-α |
― |
|
|
Rat |
Leptin |
31.5 |
|
Human |
Lepin |
＋ |
＋：存在交叉反应
―：不存在交叉反应
◆样品信息
小鼠的血清、血浆
10 μL/well（标准操作）
※样品量在10～50 μL范围内可以配制
◆测量范围
20.6～5,000 pg/mL（样品量50μL）
103~25,000 pg/mL（样品量10μL）
◆Validation data
精度测试（检测内变动系数）
|
样品 |
A |
B |
C |
|
1 |
3818 |
846 |
405 |
|
2 |
3810 |
856 |
405 |
|
3 |
3979 |
842 |
394 |
|
4 |
4047 |
851 |
392 |
|
5 |
4046 |
856 |
420 |
|
mean |
3940 |
850 |
403 |
|
SD |
118 |
6.18 |
11.2 |
|
CV(%) |
3.0 |
0.73 |
2.8 |
单位：pg/mL
再现性测试（检测内变动系数）
|
测量日/样品 |
D |
E |
|
第0天 |
5007 |
1052 |
|
第1天 |
5126 |
1063 |
|
第2天 |
5069 |
1027 |
|
第3天 |
5000 |
1000 |
|
mean |
5051 |
1035 |
|
SD |
59.3 |
28.0 |
|
CV(%) |
1.2 |
2.7 |
单位：pg/mL n=2
添加回收测试
样品F
|
添加量 |
实测值 |
回收量 |
回收率(%) |
|
0.00 |
994.9 |
– |
– |
|
337 |
1334.0 |
339.1 |
101 |
|
1061 |
2099.0 |
1104 |
104 |
|
1238 |
2284.0 |
1289 |
104 |
单位：pg/mL n=2
样品G
|
添加量 |
实测值 |
回收量 |
回收率(%) |
|
0.00 |
1996 |
– |
– |
|
2731 |
4741 |
2745 |
101 |
|
4682 |
6444 |
4448 |
95.0 |
|
5462 |
7717 |
5721 |
105 |
单位：pg/mL n=2
稀释直线性测试
用稀释缓冲液分5次连续稀释2个血清样品的测量结果，线性回归方程的R2在0.9992~0.9997之间。
欲了解更多相关产品信息，请点击文字：Lbis® 疾病相关动物模型ELISA试剂盒系列
相关资料
 |
 |
 |
| 说明书 |
ELISA试剂盒选择指南①② |
ELISA试剂盒选择指③④ |
参考文献
|
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|
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9. |
Type 2 diabetic conditions in Otsuka Long-Evans Tokushima Fatty rats are ameliorated by 5-aminolevulinic acid. Sato T, Yasuzawa T, Uesaka A, Izumi Y, Kamiya A, Tsuchiya K, Kobayashi Y, Kuwahata M, Kido Y. Nutr Res. Vol.34(6), p544-551, Jun 2014. |
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10. |
Inhibitory Effects of Japanese Herbal Medicines Sho-saiko-to and Juzen-taiho-to on Nonalcoholic Steatohepatitis in Mice. Takahashi Y, Soejima Y, Kumagai A, Watanabe M, Uozaki H, Fukusato T. PLoS One. 2014 Jan 22;9(1):e87279. |
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11. |
Down-Regulation of Hepatic Stearoyl-CoA Desaturase-1 Expression by Fucoxanthin via Leptin Signaling in Diabetic/Obese KK-A y Mice. Beppu F., Hosokawa M., Yim M-J., Shinoda T., Miyashita K. Lipids, Vol.48(5), p449-455, May 2013. |
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12. |
Dietary Combination of Fish Oil and Taurine Decreases Fat Accumulation and Ameliorates Blood Glucose Levels in Type 2 Diabetic/Obese KK-Ay Mice. N. Mikami., M. Hosokawa., K. Miyashita. Journal of Food Science, Vol. 77(6), pH114-H120, Jun 2012. |
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Effects of Gametophytes of Ecklonia Kurome on the Levels of Glucose and Triacylglycerol in db/db, Prediabetic C57BL/6J and IFN-γ KO Mice. F. Dwiranti., M. Hiraoka., T. Taguchi., Y. Konishi., M. Tominaga., A. Tominaga. Int J B 64 iomed Sci, Vol.8, No.1, Mar 2012. |
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Overexpression of FoxO1 in the Hypothalamus and Pancreas Causes Obesity and Glucose Intolerance. H.-J. Kim., M. Kobayashi., T. Sasaki., O. Kikuchi., K. Amano., T. Kitazumi., Y.-S. Lee., H. Yokota-Hashimoto., V. Y. Susanti., Y. Ido Kitamura., J. Nakae., and T. Kitamura. Endocrinology, Vol.153, No.2, p659-671, Feb 2012. |
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Spirulina improves non-alcoholic steatohepatitis, visceral fat macrophage aggregation, and serum leptin in a mouse model of metabolic syndrome. M. Fujimoto., K. Tsuneyama., T. Fujimoto., C. Selmid., M. E. Gershwin., Y. Shimada. Digestive and Liver Disease, 2012. |
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