NEBNext^® Multiplex Oligos for Illumina^® (Unique Dual Index UMI Adaptors DNA Set 3)

NEBNext^® Multiplex Oligos for Illumina^® are an essential component of the NEBNext suite of library preparation products, and are available in several different configurations for use with NEBNext products or other standard Illumina-compatible library preparation protocols. This set was designed and optimized for use in DNA sequencing workflows, including PCR-free. These unique dual index adaptors also include a 12-nt unique molecular identifier (UMI) sequence to enable either the PCR-free sample preparation for NGS, or the identification and removal of PCR errors or duplicates from amplified libraries. Supplied as 96 unique, pre-annealed index adaptors with UMIs, packaged in a single-use 96-well plate with a pierceable foil seal. Universal primers are included for applications requiring PCR amplification.

Additional NEBNext Unique Dual Index UMI Adaptors for DNA-seq are available (NEB #E7395, #E7874, #E7878) for multiplexing up to 384 samples. NEBNext Unique Dual Index UMI Adaptors for RNA-seq (NEB # E7416) are also available.

• Index adaptors enable PCR-free library preparation
• Increased sample identification specificity
• Superior and reliable ligation efficiency
• More-sensitive detection of low-frequency single-nucleotide variants (SNVs)
• Supports multiplexing for higher throughputs