Enzymatic fragmentation of DNA as part of the library prep workflow provides many advantages compared to mechanical shearing. However, specialized fragmentation reagents are required for enzymatic shearing in order to maintain methylation marks on samples for methylome analysis or for use with challenging samples such as FFPE DNA.

NEBNext UltraShear™ is a mix of enzymes that has been designed and optimized to fragment these sample types upstream of library preparation. This improves library quality and allows retention of methylation marks.

View or download extensive performance data in our Data Supplement.

• Compatible with methylation analysis workflows, including NEBNext^® Enzymatic Methyl-seq (EM-seq™) (NEB #E7120)
• Compatible with FFPE DNA
• Fast workflow with minimal hands-on time
• For methylation analysis, improves library yields, CpG coverage and sequencing metrics
• For FFPE DNA, increases usable reads and coverage uniformity and decreases artificial mutation frequency

Note that for high quality genomic DNA library prep with enzymatic fragmentation, we recommend NEBNext Ultra™ II FS DNA Library Prep for Illumina^® (NEB #E7805, #E6177).