TRAP染色试剂盒被用于破骨细胞的抗酒石酸酸性磷酸酶染色。骨量是由成骨细胞骨形成活性和破骨细胞活性之间的平衡来控制。
而碱性磷酸酶（ALP staining kit，Cat.No.PMC-AK20-COS）和抗酒石酸酸性磷酸酶分别是成骨细胞和破骨细胞的标志物。

TRAP染色试剂盒被用于破骨细胞的抗酒石酸酸性磷酸酶染色。骨量是由成骨细胞骨形成活性和破骨细胞活性之间的平衡来控制。
而碱性磷酸酶（ALP staining kit，Cat.No.PMC-AK20-COS）和抗酒石酸酸性磷酸酶分别是成骨细胞和破骨细胞的标志物。

[Protocol (96-well plate format)]
1. Remove culture medium. Wash each well once with 100 ul of PBS.
2. Add 50 ul of the 10% Formalin Neutral Buffer Solution to each well and fix for 5 minutes at room temperature.
3. Wash each well with 250 ul of deionized water. (x 3 times)
4. Dissolve 1 vial of Chromogenic Substrate with 5 ml of Tartrate-containing Buffer.
5. Add 50 ul of Chromogenic Substrates to each well.
6. Incubate at 37°C for 20-60 minutes. Adjust incubation time until stained TRAP is clearly showing the result in figure 1.
7. Wash with deionized water to stop the reaction.
Note: Excess incubation will be cause of over staining.