标记核苷酸是生命科学研究的关键要素，是分子生物学中检测特定核苷酸序列的有效工具，通过酶的作用即可结合到DNA/RNA序列中，进行测序和分析。由于出色的安全性、稳定性和便捷性，生物素和荧光标记是用于原位杂交、基因表达谱分析或电泳迁移率转移分析(EMSA)的核酸探针的最常见标记。

产品应用

核酸序列测定（Southern/Northern印记）

Hi-C技术，捕捉染色质构象

菌落与噬菌体，细胞与染色体的原位杂交

基因表达分析

治病病原体检测

遗传病与肿瘤诊断

品名：Aminoallyl-dUTP-XX-ATTO-532 Rhodamine-12-dUTP 

货号：NU-803-RHOX

Aminoallyl-dUTP-XX-ATTO532 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.

Recommended Aminoallyl-dUTP-XX-ATTO532/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-XX-ATTO532/ 50% dTTP

Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.

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