组成部分

状态

容量

(A) 抗体固相化 96 孔板

洗净后使用

96 wells（8×12）／1 块

(B) 胰岛素标准溶液（小鼠）（200 ng/mL）

稀释后使用

300 μL／1 瓶

(C) 缓冲液（蓝色）

即用

60 mL／1 瓶

(D) 生物素结合抗胰岛素抗体

稀释后使用

200 μL／1 瓶

(E) 过氧化物・抗生物素蛋白结合物

稀释后使用

200 μL／1 瓶

(F) 显色液（TMB）

即用

12 mL／1 瓶

(H) 反应终止液（1M H₂SO₄）※小心轻放

即用

12 mL／1 瓶

( I ) 浓缩洗净液（10×）

稀释后使用

100 mL／1 瓶

封板膜

3 张

使用说明书

1 份

样品

A

B

C

1

0.749

10.6

81.9

2

0.749

10.7

80.9

3

0.749

10.6

82.3

4

0.711

10.8

82.9

5

0.711

10.8

81.9

mean

0.734

10.7

82.0

SD

0.021

0.11

0.72

CV(%)

2.8

1.0

0.88

测量日/样品

F

G

H

第0天

5.61

37.3

78.9

第1天

5.85

37.9

77.9

第2天

5.65

37.6

78.2

mean

5.70

37.6

78.3

SD

0.126

0.287

0.544

CV(%)

2.2

0.8

0.7

添加量

实测值

回收量

回收率(%)

0

1.10

–

–

1.0

2.16

1.06

106

2.5

3.60

2.50

100

5.1

6.06

4.96

97.3

添加量

实测值

回收量

回收率(%)

0

31.5

–

–

20

51.5

20.0

100

40

73.1

41.6

104

60

95.1

63.6

106

ELISA试剂盒选择指南①②

1.

Does a Treadmill Running Exercise Contribute to Preventing Deterioration of Bone Mineral Density

and Bone Quality of the Femur in KK-Ay Mice, a Type 2 Diabetic Animal Model? Takagi S, Yamashita

T, Miura T. Calcif Tissue Int. 2017 Aug 4.

2.

Further characterization of diabetes mellitus and body weight loss in males of the congenic mouse

strain DDD. Cg-Ay. Suto J, Satou K. The Journal of Veterinary Medical Science, 2015.

3.

Fat and carbohydrate in western diet contribute differently to hepatic lipid accumulation.

Wu W, Tsuchida H, Kato T, Niwa H, Horikawa Y, Takeda J, Iizuka K. Biochem Biophys Res Commun.

Vol.461(4), p681-6, Jun 2015.

4.

C-C Chemokine Receptor 2 Inhibitor Ameliorates Hepatic Steatosis by Improving ER Stress and

Inflammation in a Type 2 Diabetic Mouse Model. Kim HM, Lee ES, Lee BR, Yadav D, Kim YM,

Ko HJ, Park KS, Lee EY, Chung CH. PLoS One. 2015 Mar 27;10(3):e0120711.

5.

Viability and functional assessment of murine pancreatic islets after transportation between Korea

and Japan. Lee S, Takahashi Y, Lee KM, Mizuno M, Nemeno JG, Takebe T, Lee JI. Transplant Proc.

Vol.47(3), p738-41, Apr 2015.

6.

Calorie restriction-mediated restoration of hypothalamic signal transducer and activator of

transcription 3 (STAT3) phosphorylation is not effective for lowering the body weight set point

in IRS-2 knockout obese mice. Satoko Senda,  Atsushi Inoue,  Arshad Mahmood,  Ryo Suzuki, 

Nozomu Kamei,  Naoto Kubota,  Taku Watanabe,  Masashi Aoyama,  Allah Nawaz,  Yoshiaki Ohkuma, 

Koichi Tsuneyama,  Yukiko Koshimizu,  Isao Usui,  Kumiko Saeki,  Takashi Kadowaki,  Kazuyuki Tobe.

Diabetology International February 2015

7.

Overweight in Mice and Enhanced Adipogenesis In Vitro are Associated with Lack of the Hedgehog Coreceptor Boc. Lee HJ, Jo SB, Romer AI, Lim HJ, Kim MJ, Koo SH, Krauss RS, Kang JS. Diabetes. 2015 Jan 9.

8.

Investigating the suspension culture on aggregation and function of mouse pancreatic β-cells
K.-C.Yang, C.-C.Wu, S.-H.Yang, C.-C.Chiu, S.Sumi, H.-S. Lee. Journal of Biomedical Materials Research Part A, Vol.101A(8), p2273-2282, Aug 2013.

9.

Microenvironment-regulated gene expression, morphology, and in vivo performance of mouse

pancreatic β-cells. Chen P-Y., Wu C-C., Lu D-H., Sumi S., Lin F-H., Yange K-C. Process Biochemistry,

Vol.48(1), p58-67, Jan 2013.

10.

The anti-ulcer agent, irsogladine, increases insulin secretion by MIN6 cells. T.Matsumoto，K.Sakurai,

A.Tanaka, T.Ishibashi, K.Tachibana, K.Ishikawa, K.Yokote. European Journal of Pharmacology, Vol.685

(1-3), p213-217, Jun 2012.

11.

Cell coupling regulates Ins1, Pdx-1 and MafA to promote insulin secretion in mouse pancreatic beta

cells. K.-C. Yanga., Z. Qi., G. Yanai., Y. Shirouza., D.-H. Lu., H.-S. Lee., S. Sumi. Process Biochemistry,

Vol.46(9), p1853-1860, 2011.

12.

Dietary Combination of Fish Oil and Taurine Decreases Fat Accumulation and Ameliorates Blood

Glucose Levels in Type 2 Diabetic/Obese KK-Ay Mice. N. Mikami., M. Hosokawa., K. Miyashita.

Journal of Food Science, Vol. 77(6), pH114-H120, Jun 2012.

13.

Cell coupling regulates Ins1, Pdx-1 and MafA to promote insulin secretion in mouse pancreatic beta cells. K.C. Yang., Z. Qi., G. Yanai., Y. Shirouza., D.H. Lu., H.S. Lee., and S. Sumi. Process Biochemistry.