上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
BioBrick® Assembly Kit
This product was discontinued on 12/15/2021.
Individual components can be purchased separately. Please contact [email protected].
We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. Beginning April 2021, we will be gradually transitioning to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. All information on the website has been updated to reflect this change. Find more details at www.neb.com/BSA-free.
- Catalog # E0546 was discontinued on December 15, 2021
- Assemble multi-compontent genetic systems using BioBrick parts
- BioBricks are DNA sequences that encode a defined biological function
- Easily assemble any two BioBrick parts using the same protocol
产品信息
The BioBrick Assembly Kit contains EcoRI-HF™, XbaI, SpeI, PstI, T4 DNA Ligase and NEBuffer 2.1.
- 产品类别:
- Discontinued Products
- 应用:
- BioBrick® Assembly
- 特性和用法
操作说明、说明书 & 用法
- 操作说明
- Digestion Protocol for BioBrick Assembly Kit (E0546)
- Ligation Protocol using BioBrick Assembly Kit® (E0546)
FAQs & 问题解决指南
- FAQs
- I am competing in the iGEM competition. Do you have any products that I should consider from NEB?
- Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?
引用 & 技术文献
- 引用文献
产品引用文献查找工具
Powered by Bioz See more details on Bioz
质控、安全 & 法规
- 质控分析
每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。
- CoA
CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]
- E0546S_v0_10010950
- E0546S_v0_10013772
- E0546S_v0_10023227
- E0546S_v0_10042692
- E0546S_v0_10046959
- E0546S_v0_10053289
- E0546S_v0_10054220
- E0546S_v0_10058289
- E0546S_v0_10116053
猴肿瘤浸润组织单个核细胞分离液试剂盒,货号：P4840
猴肿瘤浸润组织单个核细胞分离液试剂盒,货号：P4840
| 市场价： | ￥700.0 | |
| 价格： |
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| 品牌： | solarbio |
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产品详情
说明书下载
参考文献
|
BsaI | NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
BsaI
This product was discontinued on 12/31/2020 and replaced with the High Fidelity version, BsaI-HFv2 (NEB #R3733).
- Catalog # R0535 was discontinued on December 31, 2020
Attention Golden Gate Assembly users: BsaI-HFv2 (NEB #R3733) has been optimized for Golden Gate Assembly*. BsaI-HFv2 also works well for any protocol requiring DNA cutting by BsaI. This is the recommended enzyme for any purpose requiring digestion at the recognition sequence …5′-GGTCTC(N1)/(N5)-3′…
*The requirements for digestion of DNA during Golden Gate Assembly are more demanding than what is encountered in traditional DNA cloning. For Golden Gate, the enzyme must function well in a buffer that might not be the same buffer as that normally provided for the restriction enzyme, and maintain activity for an extended time at elevated temperatures in a dynamic cutting/re-ligating reaction with competition for substrate binding between the endonuclease and ligase. Extensive testing has demonstrated superior performance of BsaI-HFv2 compared to both BsaI (NEB #R0535) and BsaI-HF (NEB
- High Fidelity (HF®) version available () in this challenging Golden Gate assembly context where restriction enzyme efficiency and fidelity are critically important.“/products/r3733-bsai-hf-v2″>NEB #R3733) supplied with CutSmart® Buffer
- Used in Golden Gate Assembly
- Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
- 100% activity in CutSmart Buffer (over 210 enzymes are available in the same buffer) simplifying double digests
- Supplied with 1 vial of Gel Loading Dye, Purple (6X)
精选视频
产品信息
High Fidelity (HF) Restriction Enzymes have 100% activity in CutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
产品来源
An E. coli strain that carries the cloned BsaI gene from Bacillus stearothermophilus 6-55 (Z. Chen)
- 产品类别:
- Discontinued Products
- 特性和用法
单位定义
One unit is defined as the amount of enzyme required to digest 1 µg of pXba DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
反应条件
1X CutSmart® Buffer
Incubate at 37°C1X CutSmart® Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml BSA
(pH 7.9 @ 25°C)在不同缓冲液中的活性
NEBuffer™ 1.1: 75%
NEBuffer™ 2.1: 75%
NEBuffer™ 3.1: 100%稀释兼容性
- 稀释液 B
贮存溶液
10 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
500 µg/ml BSA
50% Glycerol
pH 7.4 @ 25°C热失活
65°C for 20 min
甲基化敏感性
dam 甲基化: Not Sensitive
dcm 甲基化: Impaired by Some Combinations of Overlapping
CpG甲基化: Blocked by Some Combinations of Overlapping - 相关产品
相关产品
- dam–/dcm– Competent E. coli
- Monarch® Plasmid Miniprep Kit
- Monarch® DNA Gel Extraction Kit
- Monarch® PCR & DNA Cleanup Kit (5 μg)
单独销售的组分
- Gel Loading Dye, Purple (6X)
- CutSmart® Buffer
- 注意事项
- Blocked by overlapping dcm methylation. Cleavage of mammalian genomic DNA is blocked by some combinations of overlapping CpG methylation.
- Activity at 50°C is 100%.
- May exhibit star activity in NEBuffer r1.1.
- Based on the stability of the enzyme in the reaction, incubations longerthan 1 hr will not result in improved digestion, unless additionalenzyme is added. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.
- star activity may result from a glycerol concentration of >5%
操作说明、说明书 & 用法
- 操作说明
- Optimizing Restriction Endonuclease Reactions
- Restriction Digest Protocol
- Double Digest Protocol with Standard Restriction Enzymes
- 使用指南
- Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
- Activity of Restriction Enzymes in PCR Buffers
- Cleavage Close to the End of DNA Fragments
- Dam and Dcm Methylases of E. coli
- Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
- Double Digests
- Effects of CpG Methylation on Restriction Enzyme Cleavage
- Heat Inactivation
- NEBuffer Activity/Performance Chart with Restriction Enzymes
- Optimizing Restriction Endonuclease Reactions
- Restriction Endonucleases – Survival in a Reaction
- Restriction Enzyme Diluent Buffer Compatibility
- Restriction Enzyme Tips
- Single Letter Codes
- Star Activity
- Technical Tips For Optimizing Golden Gate Assembly Reactions
- Traditional Cloning Quick Guide
- 应用实例
- Breaking through the Limitations of Golden Gate Assembly
工具 & 资源
- 选择指南
- Alphabetized List of Recognition Sequences
- Cleavage of Supercoiled DNA
- Compatible Cohesive Ends and Generation of New Restriction Sites
- Dam-Dcm and CpG Methylation
- Enzymes with Nonpalindromic Sequences
- Frequencies of Restriction Sites
- Isoelectric Points (pI) for Restriction Enzymes
- Isoschizomers
- NEB Diluent and Buffer Table
- Time-Saver™ Qualified Enzymes
- Type IIS Restriction Enzymes
- Why Choose Recombinant Enzymes?
- Web 工具
- Competitor Cross-Reference Tool
- DNA Sequences and Maps Tool
- Double Digest Finder
- Enzyme Finder
- NEBcutter™ v3.0
- NEBioCalculator®
- REBASE®
FAQs & 问题解决指南
- FAQs
- Which restriction enzymes are used in Golden Gate Assembly?
- Which restriction enzymes are used in GoldenBraid Assembly?
- Why is my Restriction Enzyme not cutting DNA?
- Why do I see a DNA smear on an agarose gel after a restriction digest?
- Why do I see additional DNA bands on my gel after a restriction digest?
- How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?
- Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
- What is the activity of the Type IIS restriction enzyme BsaI-HFv2 (NEB #R3733) in T4 DNA Ligase Buffer?
- Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
- Can Gel Loading Dye, Purple 6X (B7024) be stored in cold temperatures?
- 问题解决指南
- Restriction Enzyme Troubleshooting Guide
- 实验技巧
- Restriction Enzymes in Golden Gate Assembly
- Restriction Enzymes in Golden Gate Assembly
引用 & 技术文献
- 产品手册
- Golden Gate Assembly brochure
- 引用文献
产品引用文献查找工具
Powered by Bioz See more details on Bioz更多引用文献
- Lee JH, Won HJ, Oh E-S, Oh M-H and Jung JH (2020) Golden Gate Cloning-Compatible DNA Replicon/2A-Mediated Polycistronic Vectors for Plants Front Plant Sci; 11, 559365. DOI: 10.3389/fpls.2020.559365
- Feng Y, Zhang S, Huang X (2014) A robust TALENs system for highly efficient mammalian genome editing Sci Rep; 4, 3632. PubMedID: 24407151, DOI: 10.1038/srep03632
- Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T (2014) Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system Sci Rep; 4, 5400. PubMedID: 24954249, DOI: 10.1038/srep05400
质控、安全 & 法规
- 质控分析
每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。
- 产品说明与变更通知
产品说明与变更通知
产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]
- R0535S_L_v2
- CoA
CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]
- R0535S_L_v1_0371403
- R0535S_L_v1_0361212
- R0535S_L_v1_0361305
- R0535S_L_v1_0361311
- R0535S_L_v1_0371404
- R0535S_L_v2_0371404
- R0535S_L_v1_0371409
- R0535S_L_v2_0371503
- R0535S_L_v2_0371509
- R0535S_L_v2_0371603
- R0535S_L_v2_0381608
- R0535S_L_v2_0401702
- R0535S_L_v2_0401708
- R0535S_L_v2_0431712
- R0535S_L_v2_0431805
- R0535L_v2_10009918
- R0535S_v2_10018570
- R0535L_v2_10030542
- R0535S_v2_10030544
- R0535S_v2_10043185
- R0535L_v2_10043183
- R0535S_v2_10055060
- R0535S_v2_10059599
- R0535S_v2_10062820
- R0535L_v2_10062822
- R0535S_v2_10065817
- R0535S_v2_10081423
- R0535S_v2_10091140
- R0535L_v2_10086217
- SDS
以下 SDS 文件可以帮助您安全地使用该产品
-
BsaI
-
Allele Biotech品牌代理商
上海金畔生物科技有限公司是Allele Biotech品牌代理商 ，欢迎访问官网了解更多产品信息和订购。
Allele Biotech
Allele生物制药公司主要探索生物制药研究机制，为生物医学研究人员开发技术和产品。
Allele生物制药公司主要探索生物制药研究机制，为生物医学研究人员开发技术和产品。该公司的目标是推进科学发展，不断发现与创新。在过去十年中，Allele已经大量生产出多种分子生物学试剂，优质荧光蛋白，骆驼纳米抗体（nAb），免疫试剂盒和多种定制服务。
Allele biotechnologynology and Pharmaceuticals, Inc. is a private, San Diego-based company that explores the mechanisms of biological processes to develop technologies and products for biomedical research and therapy development.
产品列表：
|
BasoTest Kit (100 Tests) |
IDK-10-0500 |
100 Tests |
|
BasoTest Kit (20 Tests) |
IDK-10-0500S |
20 Tests |
|
MigraTest Kit (24 Tests) |
IDK-10-0800 |
24 Tests |
|
MigraTest Kit (6 Tests) |
IDK-10-0800S |
6 Tests |
|
NKTest Kit (100 Tests) |
IDK-10-0400 |
100 Tests |
|
NKTest Kit (20 Tests) |
IDK-10-0450 |
20 Tests |
|
PhagoBurst Kit – BurstTest |
IDK-10-0200S |
20 Tests |
|
PhagoBurst Kit – BurstTest |
IDK-10-0200 |
100 Tests |
|
PhagoTest Kit (100 Tests) |
IDK-10-0100 |
100 Tests |
|
PhagoTest Kit (20 Tests) |
IDK-10-0100S |
20 Tests |
PAGE浓缩胶染料（250×，黄色）
PAGE浓缩胶染料（250×，黄色）
￥50.00
货号：BL584B
规格：1mL
品牌：biosharp
Dye for PAGE Stacking Gel, 250×
PAGE浓缩胶染料（250×）
|
产品编号 |
产品名称 |
规格 |
|
BL584A |
PAGE浓缩胶染料（250×，红色） |
1 mL |
|
BL584B |
PAGE浓缩胶染料（250×，黄色） |
1 mL |
|
BL584C |
PAGE浓缩胶染料（250×，蓝色） |
1 mL |
|
BL584D |
PAGE浓缩胶染料（250×，绿色） |
1 mL |
|
BL584E |
PAGE浓缩胶染料（250×，紫色） |
1 mL |
产品简介：
本PAGE浓缩胶染料(Dye for PAGE Stacking Gel, 250×)是一种用于配制聚丙烯酰胺凝胶浓缩胶（上层胶、积成胶）的染料，该染料有红、黄、蓝、绿、紫五种颜色可供选择。添加该染料可以使得浓缩胶蛋白点样孔一目了然，方便将蛋白样品准确无误加入点样孔中，易于判断上样孔边距或是否歪曲破损。并且该染料的颜色不会随着电泳而进入分离胶（下层胶），可稳定的存在于浓缩胶中，电泳完成后也便于识别上层胶并切除，不会影响电泳和染色效果。如需凝胶配制试剂盒，可搭配使用我司产品SDS-PAGE凝胶快速制备及电泳试剂盒（BL522A），或直接购买包含彩色浓缩胶染料的凝胶快速制备试剂盒（BL563A, BL564A, BL565A, BL566A, BL567A）。
产品特点：
1. 便捷性—便于上样，易于判断上样孔边距或是否歪曲破损。
2. 稳定性—在浓缩胶中稳定，不随着电泳迁移，不影响电泳和染色结果，对后续实验没有任何影响。
使用方法：
1. 由于染料的特殊性质，在保存过程中会出现沉淀现象，因此使用前请上下颠倒混匀后即可正常使用；
2. 以1.0 mm厚凝胶为例，配制单块凝胶约需2 mL上层胶溶液，可按照1:250比例在浓缩胶溶液中加入8μL浓缩胶染料并混匀（加入颜料的多少可根据个人爱好，最大使用量不超过此建议使用量的5倍即可）；
3. 加入浓缩胶凝胶聚合催化剂（如过硫酸铵或其它过硫酸铵替代物）和TEMED，充分混匀后即可用移液管或移液器将上层胶溶液注入制胶玻璃板之间，并插入电泳梳；
4. 待上层胶凝固后，拔去电泳梳即可进行后续电泳。
注意事项：
1. 染料也可在加入凝胶聚合催化剂（如过硫酸铵或其它过硫酸铵替代物）和TEMED之后再加入，混匀后直接灌注上层胶。
2. 由于经常会出现不同样本平行电泳多块凝胶的现象，因此本公司同时提供多种颜色的浓缩胶染料，包括红色、绿色、黄色、蓝色、紫色以用于区分不同样本不同凝胶电泳。
3. 本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
4. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
保存条件：
4℃保存，有效期一年。
| 货号 | BL584B |
| 规格 | 1mL |
| 品牌 | biosharp |
| 说明书下载 | 点击下载 |
