世界*实验材料供应商 FormuMax Scientific 正式上海金畔生物为其中国代理， FormuMax Scientific 在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品，的服务，签约 FormuMax Scientific 就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获
FormuMax Scientific 中国代理, FormuMax Scientific 上海代理， FormuMax Scientific 北京代理，FormuMax Scientific 广东代理， FormuMax Scientific 江苏代理FormuMax Scientific 湖北代理,FormuMax Scientific 天津,FormuMax Scientific 黑龙江代理,FormuMax Scientific 内蒙古代理,FormuMax Scientific 吉林代理,FormuMax Scientific 福建代理, FormuMax Scientific 江苏代理,FormuMax Scientific 浙江代理, FormuMax Scientific 四川代理,
为更好的服务中国广大科研客户FormuMaxScientific从2013年开始正式上海金畔生物科技有限公司为其中国代理，为广大科研工作者带来更好的服务感受。
FormuMax Scientific公司位于美国加州硅谷，是一家致力于为药物和生物技术研究提供制剂支持的专业公司。FormuMax拥有具有世界*水平的药物传输技术团队，可以为基础研究、制剂可行性和客服定制制剂提供服务，尤其在基于脂质体制剂技术的特殊化合物传输方面有*的优势和经验。
Trans-Hi™ DNA Transfection Reagent (new)
!!! New And The Best !!!
We are very excited to announce the introduction of our brand new product, Trans-HiTM, as a superior transfection reagent to compete against the leading products in the market. Trans-HiTM is created based on a proprietary polymer technology. It forms stable complex particles with DNA and rapidly releases the DNA molecules upon endocytosis into the cytoplasm. It offers superior and reproducible transfection efficiency on the common cells such as Hela, CHO, HEK293 and many hard-to-transfect mammalian cells with minimal toxicity. Trans-HiTM is very cost-efficient and 1.0mL is sufficient for over 650 transfections in 24 well plates.
Trans-Hi™ – In vitro Transfection Reagent
SKU:
F90101TH-10
Availability:
1day
* Select volume:
- 0.5 mL
- 1.0 mL
- 10 x 1.0 mL
Product Description
Trans-Hi™ is a superior transfection reagent capable to compete against the leading products on the market. It is based on a proprietary polymer technology. It forms stable nanoparticles with DNA and rapidly releases the DNA molecules upon endocytosis into the cytoplasm. It is a broad spectrum transfection regent offering superior and reproducible transfection efficiency on the common cells such as Hela, CHO, HEK293 and many hard-to-transfect mammalian cells with minimal toxicity. Trans-Hi™ is very cost-efficient and 1.0mL is sufficient for over 660 transfections in 24 well plates.
Key Features for Trans-HiTM
- Excellent transfection for single DNA transfection or multi DNA co-transfection
- Rapid onset and long-lasting transfection
- Suitable for very long DNAs
- Simple and easy to use transfection procedure
- Robust and reproducible transfection
- High level of recombinant protein production
- Very cost efficient
- Long shelf-life: 12 months
Specifications:
| Product type: | In vitro cell transfection reagent | |
| Applications | Plasmid transfection, protein overexpression | |
| Technique: | Polymer-based DNA nanoparticles | |
| Cell type: | Established cell lines, primary cells, hard-to-transfect cells Adherent cells and suspension cells | |
| Sample type: | Plasmid DNA | |
| Serum compatibility: | Yes | |
| Product size: | 0.5, 1.0 and 10×1.0 mL | |
| Number of transfections | Up to 660 in 24-well plates with 1.0mL reagent up to 330 transfections in 6-well plates | |
| Shelf-life: | 12 months or longer when store refrigerated (2-8°C) | |
| Shipping condition: | Room temperature |
Comparison study of In vitro plasmid DNA transfection of Trans-HiTM and Lipofectamine® 2000 in HEK293T cells with GFP vector using the manufacture recommended reagent/DNA ratio of 3:1 (µL/µg) and 0.5µg DNA per well used for both products in 24 well plate 30 hours post transfection.
Trans-Hi™/DNA ratio (µL/µg) (HEK293T)
Study of the transfection efficiency as a function of transfection reagent/DNA ratio indicating optimal tranfection efficiency achieved at Trans-Hi™/DNA ratio of 3:1 (µL/µg) (HEK293T cells in 24 well plate 30 hours post transfection, 0.5ug DNA per well).
Head-to-head comparison Trans-Hi™ against Lipofectamine 2000 HEK293A cells with GFP vector (reagent/DNA ratio of 3:1(µL/µg, 0.5ug per well), 24 well plate 48 hours post transfection).
| Trans-Hi™ | Lipofectamine-2000 | |||||
| Time | Day 1 | Day 2 | Day 3 | Day 1 | Day 2 | Day 3 |
| HEK293A (%) | 79.5 | 93.4 | 93.3 | 75.7 | 89.4 | 50.6 |
Comparison study of In vitro plasmid DNA transfection of Trans-Hi™ and Lipofectamine® 2000 in Hela cells with GFP vector ((reagent/DNA ratio of 3:1(µL/µg, 0.5ug per well), 24 well plate 48 hours post transfection).
Broad Transfection Spectrum for Mammalian Cell Types
|
Cell Lines |
Efficiency (% GFP) |
|
COS-7 |
85 – 90% |
|
Hela |
85 – 90% |
|
HEK-293T |
85-90% |
|
NIH 3T3 |
75% |
|
A549 |
75% |
|
Hep3D |
65-75% |
|
SHEP |
65-70% |
|
CaCo2 |
60% |
|
HepG2 |
70% |
|
SKOV3 |
65% |
|
MCF-7 |
65-70% |
|
3T3-442A |
35-40% |
|
CHO |
80-90% |
我们公司zui大优势是强大的采购，
1：基本什么都能进口，血清，抗体，耗材，还有部分限制进口的，
2：货品全，现经营过700多个品牌，基本所有生物试剂耗材都可以进口，特别是冷偏的产品那就更有优势，
3：提供加急服务，一般1-2周到货，超过时限加急费全免
4：价格公道，绝大部分价格有优势，当然不能保证100%产品都是,因为意味着没有服务.
5：良好的信誉，大部分客户我们提供货到付款服务，客户包括清华，北大 交大 复旦，中山等100多所大学，ROCHE，阿斯利康，国药 ，fisher等500多家公司
6：我们*代理的品牌有：Antibody Research Corporation，arcticzymes，Biorelevant，AmberGen, Inc. ，clemente-associates，clodronaiposomes，Columbia Biosciences，enzyme research，Gene Bridges GmbH，Genovis，AmberGen, Inc. Biotechnology GmbH，Haematologic Technologies HTI Haemtech，hookelabs，Immudex，Innovative Research of America，inspiralis ，List Biological Laboratories, Inc.，lumafluor，Microsurfaces，multiplicom，nanotools，Pel-Freez Biologicals，pentapharm，progen，Protein Ark，QA-Bio,Inc，QA-Bio,IncQuickZyme Biosciences，Teknova，TriLink BioTechnologies, Inc.，Zyagen Laboratories 等
7：我们还是invitrogen,qiagen,Midland BioProducts Corporationam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知名*批发，欢迎合作。