mrcppureagents始于1998年，致力于协助MRC蛋白磷酸化和泛素化单位（MRC PPU）的一部分的科学家。我们自己的团队由具有分子生物学和生物化学背景的个人组成，他们经过培训可提供符合MRC预期标准的试剂PPU。
在科学中共享试剂是*的，并可以促进协作，但也可以促进发现并验证过去的结果。这些步骤有助于推动科学向前发展。MRC PPU的研究人员已经发表了前沿科学多年，并且正如预期的那样，广泛要求他们论文中使用的工具来促进其他人的工作以及整个领域。
考虑到所需试剂的数量，我们在2013年创建了一个网站，以方便访问数据库中的这些研究工具。尽管我们初的投资组合开始专注于遍在蛋白系统中的激酶，磷酸酶和酶，但我们的科学家-因此我们的试剂-已扩展到新领域。我们欢迎您查看我们不断扩大的试剂目录，以找出可能有助于您自己的研究工作的试剂。
此外，我们很乐意为研究人员提供定制生成的蛋白质或抗体的帮助。迄今为止，我们已经与来自学术界以及制药和生物技术领域的世界各地的研究人员合作完成了许多项目。请让我们知道我们将如何帮助您！
mrcppureagents cDNA克隆产品| MRC PPU试剂和服务
感兴趣的cDNA的克隆和亚克隆是分子生物学的基础，并有助于随后的生化和细胞生物学研究。尽管看似常规，但我们创建和验证克隆的方法仍保持一定程度的严格性，以确保所有克隆均符合本单位科学家所要求的准确度标准。
通过测序验证所有cDNA插入片段，然后将原始测序迹线与参考序列（Genbank）进行比对以进行验证。我们的工厂利用DNA Dynamo进行此比对。SNAPGENE，一个免费的软件程序可以打开这些文件进行查看。请参考我们的参考资料部分，以识别其他资源，这些资源可以帮助您检查和操作所需的测序文件。提供了每种构建体的详细数据表，其中列出了质粒的详细信息以及的核苷酸和蛋白质序列。
请注意：我们的投资组合中有一部分构造可能没有完整的可用数据集。这些是较旧的克隆，可在设备中使用，而当时并不一定要存储序列数据。对于要求使用这些较早构建体的任何研究人员，我们将自费对它们进行重新排序，并在发出该构建体之前生成数据表。
所有cDNA克隆都以10ul的体积提供，浓度在10-100 ng /微升之间（取决于拷贝数）-在您自己的实验室中有足够的数量用于转化和繁殖每个构建体！
| Type | Gene Name | Plasmid | Genbank | Species | Tag | Vector Type | Price per aliquot |
|---|---|---|---|---|---|---|---|
| cDNA Clones | MRCKView Data | pcDNA FRT/TO HA MRCK beta 1-473 D218A | NM_006035.3 | HA | Mammalian | £110.00 | |
| cDNA Clones | UVRAGView Data | pcDNA5D FRT/TO 3xflag UVRAG S508A | Human | FLAG | Mammalian | £110.00 | |
| cDNA Clones | UVRAGView Data | pcDNA5D FRT/TO 3xFlag UVRAG S508A S509A | Human | FLAG | Mammalian | £110.00 | |
| cDNA Clones | (chicken) CDKL5View Data | pcDNA5D FRT/TO Chicken CDKL5 TEV GST 6His | Sequence provided by Michael Morgan | Chicken | GST | Mammalian | £110.00 |
| cDNA Clones | (chicken) CDKL5View Data | pEX Chicken CDKL5 TEV GST 6His | Sequence provided by Michael Morgan. | Chicken | GST, 6His | Bacterial | £110.00 |
| cDNA Clones | (TEV)-Ubiquitin-UbiquitinView Data | pGST-1-(blunt)TEV_diubiquitin | P62987 | Human | GST | Bacterial | £110.00 |
| cDNA Clones | (TEV)-Ubiquitin-UbiquitinView Data | pSc-B-(blunt)TEV_diubiquitin | P62987 | Other | £110.00 | ||
| cDNA Clones | -View Data | pSuperior Neo GFP- shRNA-NegativeControl | – | GFP | Mammalian | £110.00 | |
| cDNA Clones | 132View Data | pGL4.13 132 comp (1 copy) | Mammalian | £110.00 | |||
| cDNA Clones | 13376836View Data | pCMV-HA 13376836 | NM_025232 | Human | HA | Mammalian | £110.00 |
| cDNA Clones | 13376836View Data | pGEX 1337836 | NM_025232 | Human | GST | Bacterial | £110.00 |
| cDNA Clones | 13376836View Data | TOPO-13376836 | Other | £110.00 | |||
| cDNA Clones | 13376836 (presenilin like)View Data | pGFP-HA 13376836 | NM_025232 | Human | GFP, HA | Mammalian | £110.00 |
| cDNA Clones | 14-3-3 betaView Data | pcDNA5-FRT/TO-mCherry 14-3-3 beta | XM_017028039.1 | Human | mCherry | Mammalian | £110.00 |
| cDNA Clones | 14-3-3 betaView Data | pEBG6P-1 14-3-3 beta | NM_139323.2 | Human | GST | Mammalian | £110.00 |
| cDNA Clones | 14-3-3 betaView Data | pET28a 14-3-3 beta | NP_647539 | Human | 6His | Bacterial | £110.00 |
| cDNA Clones | 14-3-3 betaView Data | pGEX6P-14-3-3 beta | NM_139323.2 | Human | GST | Bacterial | £110.00 |
| cDNA Clones | 14-3-3 beta (mouse)View Data | pBluescript KS+mouse 14-3-3 beta | NM_018753.6 GI:158508429 with silent change g588a | Mouse | Other | £110.00 | |
| cDNA Clones | 14-3-3 epsilonView Data | pBabe hygro FLAG-mCherry 14-3-3 epsilon | Human | FLAG, mCherry | Mammalian | £110.00 | |
| cDNA Clones | 14-3-3 epsilonView Data | pcDNA5-FRT/TO NRL-14-3-3 epsilon | Mammalian | £110.00 | |||
| cDNA Clones | 14-3-3 epsilonView Data | pcDNA5-FRT/TO-mCherry 14-3-3 epsilon | mCherry | Mammalian | £110.00 | ||
| cDNA Clones | 14-3-3 epsilonView Data | pcDNA5-FRT/TO-mCherry 14-3-3 epsilon | mCherry | Mammalian | £110.00 | ||
| cDNA Clones | 14-3-3 epsilonView Data | pEBG-6 14-3-3 epsilon | HSU54778 | GST | Mammalian | £110.00 | |
| cDNA Clones | 14-3-3 epsilonView Data | pET28a 14-3-3 epsilon | NP_006752.1 | Human | 6His | Bacterial | £110.00 |
| cDNA Clones | 14-3-3 epsilonView Data | pGEX 14-3-3 epsilon Y211/K | GST | Bacterial | £110.00 | ||
| cDNA Clones | 14-3-3 epsilonView Data | pGEX 14-3-3 epsilon E183/K | GST | Bacterial | £110.00 | ||
| cDNA Clones | 14-3-3 epsilonView Data | pGEX 14-3-3 epsilon F199/Y | GST | Bacterial | £110.00 | ||
| cDNA Clones | 14-3-3 epsilonView Data | pGEX-6-14-3-3 epsilon | HSU54778 | Human | GST | Bacterial | £110.00 |
| cDNA Clones | 14-3-3 epsilonView Data | pIRES2-mCherry 14-3-3 epsilon/pEGFP | NM_006761.4 GI:195546907 | GFP | Mammalian | £110.00 | |
| cDNA Clones | 14-3-3 epsilonView Data | pJET-m-cherry 14-3-3 epsilon | Other | £110.00 | |||
| cDNA Clones | 14-3-3 epsilonView Data | TOPO-14-3-3 epsilon | HSU54778 | Human | Other | £110.00 | |
| cDNA Clones | 14-3-3 epsilon (mouse)View Data | pBluescript KS+ mouse 14-3-3 epsilon | NM_009536.4 GI:226874905 with silent changes c639t and t711c | Mouse | Other | £110.00 | |
| cDNA Clones | 14-3-3 etaView Data | Hygro-retrovir-FLAG-mCherry 14-3-3 eta | Human | FLAG, mCherry | Mammalian | £110.00 | |
| cDNA Clones | 14-3-3 etaView Data | pcDNA5-FRT/TO-mCherry 14-3-3 eta | mCherry | Mammalian | £110.00 | ||
| cDNA Clones | 14-3-3 etaView Data | pEBG6P-14-3-3 eta | NP_003396.1 | Human | GST | Mammalian | £110.00 |
| cDNA Clones | 14-3-3 etaView Data | pET28a 14-3-3 eta | NP_003396.1 | Human | 6His | Bacterial | £110.00 |
| cDNA Clones | 14-3-3 etaView Data | pET28a 14-3-3 sigma | NP_006133.1 | Human | 6His | Bacterial | £110.00 |
| cDNA Clones | 14-3-3 etaView Data | pGEX6P-1-14-3-3 eta | NP_003396.1 | Human | GST | Bacterial | £110.00 |
| cDNA Clones | 14-3-3 etaView Data | pJET-m-cherry 14-3-3 eta (Bgl2-Xho1) | Other | £110.00 | |||
| cDNA Clones | 14-3-3 eta (mouse)View Data | pBluescript KS+ mouse 14-3-3 eta | NM_011738.2 GI:162951868 | Mouse | Other | £110.00 | |
| cDNA Clones | 14-3-3 gammaView Data | Hygro-retrovir-FLAG-mCherry 14-3-3 gamma | Human | FLAG, mCherry | Mammalian | £110.00 | |
| cDNA Clones | 14-3-3 gammaView Data | pcDNA5-FRT/TO-mCherry 14-3-3 gamma | mCherry | Mammalian | £110.00 | ||
| cDNA Clones | 14-3-3 gammaView Data | pEBG6P-14-3-3 gamma | AAH20963.1 | Human | GST | Mammalian | £110.00 |
| cDNA Clones | 14-3-3 gammaView Data | pET28a 14-3-3 gamma | AAH20963.1 | Human | 6His | Bacterial | £110.00 |
| cDNA Clones | 14-3-3 gammaView Data | pGEX6P-1-14-3-3 gamma | AAH20963.1 | Human | GST | Bacterial | £110.00 |
| cDNA Clones | 14-3-3 gammaView Data | pJET-m-cherry 14-3-3 gamma | Other | £110.00 | |||
| cDNA Clones | 14-3-3 gamma (mouse)View Data | pBluescript KS+ mouse 14-3-3 gamma | NM_018871.3 GI:229577260 | Mouse | Other | £110.00 | |
| cDNA Clones | 14-3-3 sigmaView Data | Hygro-retrovir-FLAG-mCherry 14-3-3 sigma | Human | FLAG, mCherry | Mammalian | £110.00 | |
| cDNA Clones | 14-3-3 sigmaView Data | pcDNA5-FRT/TO-mCherry 14-3-3 sigma | mCherry | Mammalian | £110.00 | ||
| cDNA Clones | 14-3-3 sigmaView Data | pCMV5-FLAG 14-3-3 sigma | AF029081 | Human | FLAG | Mammalian | £110.00 |