人类原代细胞一览

人类原代细胞一览

推荐的培养基* 产品 接种密度 溶解后传代 标记物** 种群加倍
C-22070 人类心肌细胞 (HCM) 10,000 – 15,000
cells per cm²
P2 Sarcomeric alpha-Actinin+
Slow muscle myosin+
> 15
C-27101 人类软骨细胞(HCH) 10,000 – 20,000
cells per cm²
P2 Differentiation tested > 10
C-22010
C-22011
人类脐静脉内皮细胞(HUVEC) 5,000 – 10,000
cells per cm²
P1 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22010
C-22011
人类脐静脉内皮细胞(HUVEC) 混合 5,000 – 10,000
cells per cm²
P1 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22010
C-22011
人类脐静脉内皮细胞 (HUVEC)
预先筛选
5,000 – 10,000
cells per cm²
P1 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22010
C-22011
人类脐动脉内皮细胞 (HUAEC) 5,000 – 10,000
cells per cm²
P1 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22020
C-22022
人类主动脉内皮细胞 (HAoEC) 5,000 – 10,000
cells per cm²
P2 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22020
C-22022
人类冠状动脉内皮细胞 (HCAEC) 5,000 – 10,000
cells per cm²
P2 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22010
C-22011
人类肺动脉内皮细胞 (HPAEC) 5,000 – 10,000
cells per cm²
P2 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22010
C-22011
人类大隐静脉内皮细胞 (HSaVEC) 5,000 – 10,000
cells per cm²
P2 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22020
C-22022
人类皮肤微血管内皮细胞 (HDMEC)
青少年包皮
10,000 – 20,000
cells per cm²
P2 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22020
C-22022
人类皮肤微血管内皮细胞 (HDMEC)
成年人供者
10,000 – 20,000
cells per cm²
P2 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22020
C-22022
人类皮肤微血管内皮细胞 (HDMEC)
预先筛选
10,000 – 20,000
cells per cm²
P2 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22020 人类皮肤血液内皮细胞 (HDBEC)
青少年包皮
10,000 – 20,000
cells per cm²
P2 Podoplanin                                
Prox-1
CD31+
> 15
C-22020 人类皮肤血液内皮细胞 (HDBEC)
成年人供者
10,000 – 20,000
cells per cm²
P2 Podoplanin                                
Prox-1
CD31+
> 15
C-22020 人类皮肤淋巴内皮细胞 (HDLEC)
青少年包皮
10,000 – 20,000
cells per cm²
P2 Podoplanin                                
Prox-1
CD31+
> 15
C-22020 人类皮肤淋巴内皮细胞 (HDLEC)
成年人供者
10,000 – 20,000
cells per cm²
P2 Podoplanin                                
Prox-1
CD31+
> 15
C-22020
C-22022
人类膀胱微血管内皮细胞 (HBdMEC) 10,000 – 20,000
cells per cm²
P2 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22020
C-22022
人类心脏微血管内皮细胞 (HCMEC) 10,000 – 20,000
cells per cm²
P2 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22020
C-22022
人类肺脏微血管内皮细胞 (HPMEC) 10,000 – 20,000
cells per cm²
P2 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22020
C-22022
人类子宫微血管内皮细胞 (HUtMEC) 10,000 – 20,000
cells per cm²
P2 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-21060 人类鼻部上皮细胞 (HNEpC) 10,000 – 15,000
cells per cm²
P2 Cytokeratin+ > 15
C-21060 人类气管上皮细胞 (HTEpC) 10,000 – 15,000
cells per cm²
P2 Cytokeratin+ > 15
C-21060 人类支气管上皮细胞 (HBEpC) 10,000 – 15,000
cells per cm²
P2 Cytokeratin+ > 15
C-21070 人类小气道上皮细胞 (HSAEpC) 10,000 – 15,000
cells per cm²
P2 Cytokeratin+ > 15
C-21010 人类乳腺上皮细胞 (HMEpC) 10,000 – 15,000
cells per cm²
P2 Cytokeratin+ > 15
C-26001
C-26030
人类肾脏上皮细胞 (HREpC) 10,000 – 15,000
cells per cm²
P2 Cytokeratin+ > 15
C-26001
C-26030
人类肾皮质上皮细胞 (HRCEpC) 10,000 – 15,000
cells per cm²
P2 Cytokeratin+ > 15
C-26040 人类胎盘上皮细胞 (HPlEpC) 10,000 – 15,000
cells per cm²
P2 Cytokeratin+ > 5
C-23010 正常人类皮肤成纤维细胞 (NHDF)
青少年包皮
3,500 – 7,000
cells per cm²
P2 CD90+ > 15
C-23020 正常人类皮肤成纤维细胞 (NHDF)
成年人供者
3,500 – 7,000
cells per cm²
P2 CD90+ > 15
C-23020 人类肺脏成纤维细胞 (HPF) 3,500 – 7,000
cells per cm²
P2 CD90+ > 15
C-23020 人类主动脉外膜成纤维细胞 (HAoAF) 3,500 – 7,000
cells per cm²
P2 CD90+ > 10
C-23025 人类心脏成纤维细胞 (HCF) 3,500 – 7,000
cells per cm²
P2 CD90+                                     
Smooth muscle α-actin           
Slow muscle myosin
> 15
C-23020 人类子宫成纤维细胞 (HUF) 3,500 – 7,000
cells per cm²
P2 CD90+ > 15
C-23020 人类绒毛膜间充质成纤维细胞 (HVMF) 3,500 – 7,000
cells per cm²
P2 CD90+ > 15
C-26501 人类毛囊毛乳头细胞 (HFDPC) 5,000 – 10,000
cells per cm²
P2 Alkaline phosphatase+ > 10
C-25010
C-25020
人类肝脏细胞 (HHpC) 150,000 cells per cm² P0 ECOD+                                     
7-HCG/7-HCS+          
Albumin+
 
C-20011 正常人类表皮角质化细胞 (NHEK)
青少年包皮, 单一供者
5,000 cells per cm² P2 Cytokeratin+ > 15
C-20011 正常人类表皮角质化细胞 (NHEK) 青少年包皮,混合 5,000 cells per cm² P2 Cytokeratin+ > 15
C-20011 正常人类表皮角质化细胞 (NHEK)
成年人单一供者
5,000 cells per cm² P2 Cytokeratin+ > 15
C-20011 正常人类表皮角质化细胞 (NHEK)
成年人供者, 混合
5,000 cells per cm² P2 Cytokeratin+ > 15
C-24010 正常人类表皮黑色素细胞 (NHEM)
青少年包皮
5,000 – 10,000
cells per cm²
P2 Mel-5+ > 15
C-24300 正常人类表皮黑色素细胞 (NHEM)
青少年包皮,培养于M2培养基
5,000 – 10,000
cells per cm²
P2 Mel-5+ > 15
C-24300 正常人类表皮黑色素细胞 (NHEM)
成年人供者,培养于M2培养基
5,000 – 10,000
cells per cm²
P2 Mel-5+ > 15
C-27001 人类成骨细胞 (HOB) 10,000 – 20,000
cells per cm²
P2 Alkaline phosphatase+
Mineralization tested
> 10
C-27410
C-27436
C-27438
人类白色前成脂肪细胞 (HWP)
皮下脂肪
5,000
cells per cm²
P2 Differentiation tested > 10
C-27410
C-27436
C-27438
人类白色前成脂肪细胞 (HWP)
内脏脂肪
5,000
cells per cm²
P2 Differentiation tested > 10
C-23060
C-23061
人类骨骼肌细胞
(SkMC)
3,500 – 7,000
cells per cm²
P2 Sarcomeric Myosin+
Differentiation tested
> 15
C-22062 人类主动脉平滑肌细胞
(HAoSMC)
7,500 – 10,000
cells per cm²
P2 Smooth muscle alpha-Actin+ > 15
C-22062 人类冠状动脉平滑肌细胞 (HCASMC) 7,500 – 10,000
cells per cm²
P2 Smooth muscle alpha-Actin+ > 15
C-22062 人类肺动脉平滑肌细胞
(HPASMC)
7,500 – 10,000
cells per cm²
P2 Smooth muscle alpha-Actin+ > 15
C-22062 人类脐带动脉平滑肌细胞 (HUASMC) 7,500 – 10,000
cells per cm²
P2 Smooth muscle alpha-Actin+ > 15
C-22062 人类气管平滑肌细胞 (HTSMC) 7,500 – 10,000
cells per cm²
P2 Smooth muscle alpha-Actin+ > 15
C-22062 人类支气管平滑肌细胞(HBSMC) 7,500 – 10,000
cells per cm²
P2 Smooth muscle alpha-Actin+ > 15
C-22062 人类膀胱平滑肌细胞 (HBdSMC) 7,500 – 10,000
cells per cm²
P2 Smooth muscle alpha-Actin+ > 15
C-22062 人类前列腺平滑肌细胞 (HPrSMC) 7,500 – 10,000
cells per cm²
P2 Smooth muscle alpha-Actin+ > 15
C-22062 人类子宫平滑肌细胞
(HUtSMC)
7,500 – 10,000
cells per cm²
P2 Smooth muscle alpha-Actin+ > 15

* 该表格中的培养基为即用型。培养基试剂盒信息,请参见第4章。
** CD 31, Prox-1 和肾小球上皮细胞整合膜蛋白抗体均可以从 PromoKine购买。

 

  • 心肌细胞
  • 软骨细胞
  • 内皮细胞(大血管)
  • 内皮细胞(微血管)
  • 上皮细胞
  • 成纤维细胞
  • 毛囊毛乳头细胞
  • 肝细胞

脐带间充质干细胞无血清培养基(原代),货号:NC0103+NC0103.S

脐带间充质干细胞无血清培养基(原代),货号:NC0103+NC0103.S

市场价: 1890.0
价格:
1606.50
品牌: 友康
规格: 500ml*1+5ml添加剂
产品详情

说明书下载

参考文献

脐带间充质干细胞无血清培养基,货号:NC0103+NC0103.S


简要描述:

间充质干细胞无血清培养基(脐带—原代细胞分离及种子库构建)既用于脐带间充质干细胞的原代分离,也可以用于种子库构建。可稳定传至10代。 间充质干细胞无血清培养基的优点:未添加血清且无动物源成分;不含任何未知组分物质;产品批间差小。






【间充质干细胞无血清培养基(脐带—原代细胞分离及种子库构建)   用途与描述】
间充质干细胞无血清培养基(脐带—原代细胞分离及种子库构建)用于脐带间充质干细胞的原代细胞分离及种子库的构建,同时还能保持其多向分化的潜能,如脐带(UCM-hMSC)。
使用本产品无需添加血清或血清替代物。
间充质干细胞无血清培养基化学成分明确、无血清、无动物源成分。产品批间差异小,所有原料符合GMP标准。更适合临床研究用途。

【间充质干细胞无血清培养基(脐带—原代细胞分离及种子库构建)   规格与保存】

脐带间充质干细胞无血清培养基(原代),货号:NC0103+NC0103.S

MSC无血清培养基中没有添加胰酶抑制剂,建议使用干细胞温和消化酶消化,细胞变圆后,直接加细胞培养上清稀释即可,无需终止消化。

【间充质干细胞无血清培养基(脐带—原代细胞分离及种子库构建)   产品性能】

原代细胞收获

应用友康生物的脐带原代细胞分离技术,结合无血清培养基,组织块克隆率可达到100%以上,优质脐带样本可达到300%。

统计数据表明:应用友康生物的脐带原代细胞分离技术,结合无血清培养基一根20cm的符合条件的脐带,大致可收获原代细胞2.4×107个。

原代细胞爬出及收获时间:

脐带间充质干细胞无血清培养基(原代),货号:NC0103+NC0103.S

传代细胞收获

将从一根可用部分为16cm的脐带为例,连续传代细胞的均为经冻存复苏操作,所有步骤均严格遵循友康技术工艺,可收获
P3代细胞: 9496×107

P4代细胞: 108907×107

P5代细胞: 949409×107

脐带间充质干细胞无血清培养基(原代),货号:NC0103+NC0103.S

细胞形态

脐带间充质干细胞无血清培养基(原代),货号:NC0103+NC0103.S    脐带间充质干细胞无血清培养基(原代),货号:NC0103+NC0103.S

    脐带间充质干细胞无血清培养基(原代),货号:NC0103+NC0103.S脐带间充质干细胞无血清培养基(原代),货号:NC0103+NC0103.S

细胞免疫表型

脐带间充质干细胞无血清培养基(原代),货号:NC0103+NC0103.S

脐带间充质干细胞无血清培养基(原代),货号:NC0103+NC0103.S

脐带间充质干细胞无血清培养基(原代),货号:NC0103+NC0103.S

细胞诱导分化

脐带间充质干细胞无血清培养基(原代),货号:NC0103+NC0103.S


* 以上数据为应用友康生物脐带分离技术,无血清培养基及干细胞温和消化酶所得数据。

不同脐带样本与不同的分离技术,会导致较大的结果差异。

客户问答

三代培养基的差别

培养基类型
血清培养基(1代)
血清替代物培养基(2代)
无血清培养基(3代)
是否有明确的配方
是否无动物源
产品批间差


接种密度/cm2 5000-10000 5000-10000 8000
消化时间 0.25%胰酶/3min 0.25%胰酶/2-3min 干细胞温和消化酶/2-5min
终止消化方式 完全培养基 完全培养基 不需终止,直接加细胞培养上清稀释


Cell application 原代细胞 / 培养基

Cell application 原代细胞 / 培养基 / 细胞培养试剂

Cell Applications(CA,细胞应用公司)是一家总部位于美国的生物技术公司,专注于提供高质量的细胞和细胞相关产品,以支持生命科学研究和应用。该公司的产品和服务覆盖了细胞培养、分子生物学、蛋白质组学、细胞分析和药物筛选等广泛领域。

Cell applications常卖产品:

货号 品名 规格 品牌
128-100 Bovine Insulin tested for cell culture 100mg cellapplications
080-100 Trypsin Neutralization Solution: Stops the trypsinization reaction. 100ml cellapplications
062-100 HBSS: 1X HBSS for washing of cells before Trypsin/EDTA treatment 100ml cellapplications
070-100 Trypsin/EDTA Solution: For subculturing of adherent cells. 100ml cellapplications
022K DiI-Ac-LDL Kit 1Kit cellapplications
408K-05a Cryopreserved HFLS, Adult: 5×10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) 1Kit cellapplications
411-500 Chondrocyte Growth Medium 500ml cellapplications
415-GS HFLS Growth Supplement 50ml cellapplications
415K-500 HFLS Growth Medium Kit Yields 500 ml cellapplications
511-500 HBEpC/HTEpC Growth Medium 500ml cellapplications
511D-250 HBEpC/HTEpC Differentiation Medium 250ml cellapplications
602k-05a Human Hair Follicle Dermal Papilla Cells kit cellapplications
811D-250 HPAd Differentiation Medium 250ml cellapplications
R352-05 Rat Pulmonary Artery Smooth Muscle Cells (RPASMC) 1 Cryovial cellapplications
TF102K Cytofect™ Epithelial Cell Transfection Kit 1Kit cellapplications

关键词:Cell Applications;Cell Applications 128-100;128-100;Bovine Insulin tested for cell culture;细胞

Evercyte原代细胞

Evercyte原代细胞

Evercyte公司的主要业务包括:细胞系的开发、生产和销售,涵盖了各种类型的人类细胞,如肾上腺细胞、肾细胞、肝细胞、肺细胞、神经元等,这些细胞系被广泛应用于药物筛选、毒性测试、细胞治疗、基因治疗和生物医学研究等领域。

Evercyte常卖产品:

货号 品名 规格 品牌
CHT-003-0002 Kidney RPTEC/TERT1 1vial evercyte
CHT-031-0166 fHDF/TERT166 1vial evercyte
CkHT-040-231-2 Muscle LHCN-M2 ea evercyte
MHT-033-3 PodoUp3 ready to use 500ml evercyte
MHT-033-3-B+MHT-033-3-S Kidney PodoUp3 KIT evercyte
MHT-040 MyoUp ready to use 500ml evercyte

关键词:Evercyte;Evercyte代理;CHT-003-0002;CHT-031-0166;CkHT-040-231-2

Zen-Bio细胞库/细胞培养/原代细胞

Zen-Bio细胞库/细胞培养/原代细胞


Zen-Bio是一家成立于1995年的生物技术公司,总部位于美国北卡罗来纳州瑞利市。该公司专注于开发和提供高质量的细胞、组织和血清产品,为生命科学领域的研究人员和制药公司提供支持。Zen-Bio的产品广泛应用于肥胖研究、糖尿病、心血管疾病、皮肤病、神经退行性疾病和癌症等疾病的研究。Zen-Bio的细胞和组织产品包括皮下脂肪、脂肪组织、骨髓间充质干细胞、皮肤、心脏、肝脏、胰腺、骨骼肌等。同时,该公司还提供不同种类的血清产品,包括胎牛血清、成年牛血清、羊血清等,可以满足客户不同的需求。除此之外,Zen-Bio还提供客户定制服务,满足客户特殊需求。

zen-bio常卖产品:

货号 品名 规格 品牌
DM-2-L1 3T3-L1 Differentiation Medium, 100ml 100ml zen-bio
PM-1-L1 3T3-L1 Preadipocyte Medium, 500ml 500ml zen-bio
LIP-2-NC-L1 3T3-L1 Lipolysis Assay Kit (free fatty acids detection; REAGENTS ONLY) Kit zen-bio
CO-5 LIP-2/3 Assay Buffer 200ml zen-bio
AM-1-L1 3T3-L1 Adipocyte Maintenance Medium, 500ml 500ml zen-bio
SP-F-2 Cryopreserved, Subcutaneous Preadipocytes (2 million cells/vial) BMI 25.0-29.99 2millioncells/vial zen-bio
SP-F-1 Cryopreserved, Subcutaneous Preadipocytes (2 million cells/vial) BMI<24.99 2millioncells/vial zen-bio
SKM-M Skeletal Muscle Cell Growth Medium, 500ml 500ml zen-bio
SP-F-3 Cryopreserved, Subcutaneous Preadipocytes (2 million cells/vial) BMI >30.0 2millioncells/via zen-bio
SKM-D Skeletal Muscle Cell Differentiation Medium, 500ml 500ml zen-bio
SKB-F-SL Cryopreserved, Human Skeletal Myoblasts (.5×106 cells), POOLED Donor lot EACH zen-bio
OP-F-2 Cryopreserved, omental preadipocytes (1×106 cells). BMI 25-29.99 Vial zen-bio
SER-PMN Human Polymorphonuclear Cells (PMN), Cryopreserved, 15 million cells/vial 15M zen-bio
SER-PLP Human Recovered Plasma, FROZEN POOLED (Any ANTICOAGU-LANT) 200ml zen-bio
SER-PLE-ZB human recovered plasma zen-bio
SER-PBMC-F Human Peripheral Blood Mononuclear Cells, Cryopreserved 15millioncells/vial zen-bio
SER-CD34-MPB1-F Human Mobilized Peripheral Blood CD34+ Cells, Cryopreserved 1millioncells/vial zen-bio
SER-CD14-F Normal Human Peripheral Blood CD14+ MONOCYTES, Cryopreserved 5millioncells/vial zen-bio
SP-F-SL Cryopreserved, Subcutaneous Preadipocytes, POOLED Lot (2 million cells/vial) BMI 25.0-29.99 2millioncells/vial zen-bio
380790 alpha 1 Sodium Potassium ATPase Rabbit pAb 50ul zen-bio
OP-F-1 Cryopreserved, omental preadipocytes (1×106 cells). BMI <25.0 Vial zen-bio
LIP-6-NC Adipose Tissue Explant Lipolysis Assay Kit: Glycerol Detection KIT zen-bio
FM-1-100 Preadipocyte Cryopreservation medium 100ml zen-bio
EZPREP-10 EZPREP Peripheral Blood Mononuclear Cell Isolation Kit (can process up to 100ml of blood).+ Includes EZPREP Tubes and Reagents KIT zen-bio
DM-2-500 Subcutaneous Preadipocyte Differentiation Medium 500ml zen-bio
CnT-57 PCT Epidermal Keratinocyte Medium, Low BPE 500mLKIT zen-bio
BM-1 Subcutaneous Basal Medium 500ml zen-bio
AOX-17 Total Phenolic Content Assay Kit Kit zen-bio
PM-1 Subcutaneous Preadipocyte Medium 500ml zen-bio
DM-2 Subcutaneous Preadipocyte Differentiation Medium 100ml zen-bio
AM-1 Subcutaneous Adipocyte Maintenance Medium 500ml zen-bio

关键词:Zen-Bio;Zen-Bio代理;DM-2-L1;PM-1-L1;LIP-2-NC-L1