worthington木瓜蛋白酶产品
木瓜蛋白酶产品信息
来源: Carica Papaya Latex
IUB:3.4.22.2
木瓜蛋白酶是来自Carica papaya乳胶的巯基蛋白酶。它的分子量为23kDa,适pH范围为6.0-7.0。木瓜蛋白酶对亮氨酸甲酯的作用产生不溶性聚亮氨酸肽。木瓜蛋白酶分解软骨的细胞间基质。木瓜蛋白酶被半胱氨酸,硫化物和亚硫酸盐活化。稳定剂是EDTA,半胱氨酸和二巯基乙醇。
稳定性/储存: 在2-8°C下稳定6-12个月。不要冷冻水悬浮液。
技术注释:木瓜蛋白酶制剂应在使用前在活化溶液中培养,以确保*活动。应用包括抗体片段化和原代/神经细胞分离。
单位定义:在含有1.1mM EDTA,0.067mM巯基乙醇和5.5mM半胱氨酸-HCl的溶液中活化30分钟后,一个单元在25℃,pH6.2下每分钟水解一微摩尔苯甲酰-L-精氨酸乙酯。
相关产品:
胶原酶(CLS1-4 / CLSPA)
脱氧核糖核酸酶I(DP / D / DCLS / D2 / DPFF / DPRF)
透明质酸
酶(HSE / HSEP)中性蛋白酶(Dispase,NPRO)
蛋白酶K(PROK)
胰蛋白酶(TL / TRL / TRL3 / TRLS) / TRTPCK)
胰蛋白酶抑制剂(LBI / OI / SI / SIC)
细胞分离优化系统(CIT)
肝细胞分离系统(HIS)
新生儿心肌细胞分离系统(NCIS)
木瓜蛋白酶解离系统(PDS / PDS2)
Papain, Suspension Supplied as a two times crystalline suspension in 50mM sodium acetate, pH 4.5. To ensure full activity, the enzyme should be incubated in a solution containing 1.1mM EDTA, 0.067mM mercaptoethanol and 5.5mM cysteine-HCl for 30 minutes. It is recommended that the enzyme be 0.22 micron filtered after dissolution and prior to use. Store at 2-8°C. REQUIRES SPECIAL SHIPPING: ICE PACK
木瓜蛋白酶,悬浮液为两次结晶悬浮液,加入50 mm醋酸钠,pH4.5。为确保酶的充分活性,酶应在含有1.1MM EDTA,0.067mM的溶液中孵育。 巯基乙醇和5.5MM半胱氨酸-盐酸作用30分钟。建议酶在溶解后和使用前过滤0.22微米。储存温度2至8度C.需要特殊运输:冰袋
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Activates to at least 20 units per mg protein
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25 mg
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LS003124
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100 mg
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LS003126
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1 gm
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LS003127
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|
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Bulk
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LS003128
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Papain, Lyophilized Supplied as a lyophilized powder prepared from a two times crystalline suspension, Code: PAP. To insure full activity, the enzyme should be incubated in a solution containing 1.1mM EDTA, 0.067mM mercaptoethanol and 5.5mM cysteine-HCl for 30 minutes. It is recommended that the enzyme be 0.22 micron filtered after dissolution and prior to use. Store at 2-8°C.
木瓜蛋白酶,冻干供应的冻干粉由两次结晶悬浮制备,代码:PAP。为了确保酶的充分活性,酶应在含有1.1米的溶液中孵育。 m EDTA、0.067mM巯基乙醇和5.5MM半胱氨酸-HCl作用30 min。建议酶在溶解后和使用前过滤0.22微米。贮藏温度2~8°C。
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Activates to at least 15 units per mg protein
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25 mg
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LS003118
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100 mg
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LS003119
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1 gm
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LS003120
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Bulk
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LS003122
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PDS Kit, Papain Vial A component of the Papain Dissociation System, for use in the tissue dissociation method of Huettner, J.E., and Baughman, R.W.: J. Neuroscience, 6, 3044 (1986). Contains papain, L-cysteine, and EDTA. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5 ml of EBSS or equivalent yields a solution at 20 units of papain per ml in 1mM L-cysteine with 0.5mM EDTA. Store at 2-8°C.
PDS Kit,Papain Vial A组分,木瓜蛋白酶解离系统,用于Huettner,J.E.和Baughman的组织解离方法,R.W.:J.神经科学,6,3044(1986)。含有木瓜蛋白酶 半胱氨酸和EDTA。这种材料是0.22微米膜过滤和冻干在蒸压瓶。用5毫升的EBSS或同等物重组一小瓶,可产生20个单位的木瓜蛋白酶p溶液。 在1mm L-半胱氨酸中加入0.5mm EDTA。贮藏温度2~8°C。
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≥100 units per vial
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1 vi
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LK003176
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5 vi
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LK003178
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