Probetex肾毒性抗血清说明书

Probetex,Inc.是一家成立于1997年的生命科学与合同研究组织(CRO),该公司列出了六个专业领域,其中包括:1)成人和胚胎肾细胞系的细胞库;2)产生和销售绵羊肾毒性抗体,以诱导抗肾小球基底膜(GBM)肾小球肾炎,膜性(Fx1A)肾病和血管增生性(Thy1)肾小球肾炎。3)来自肾脏疾病模型的组织产品;4)组织病理学服务,提供常规组织学,免疫组织化学,电子显微镜和图像分析;5)专门从事上述模型以及其他20多种急慢性实验性肾脏病,心血管肝和肺病模型的定制合同研究; Probetex还协助评估和开发罕见疾病模型。 

上海金畔生物Probetex肾毒性抗血清说明书

  • Probetex PTX-001AGBM:绵羊抗大鼠分离肾小球基底膜(抗GBM)血清

用于诱导免疫介导的肾小球肾炎。

  •    系膜增生
  •    炎性细胞血管炎
  •    肾小球硬化

   肾纤维化
注意:该产品替代了以前的PTX-001S绵羊抗全大鼠肾小球。两种产品均针对大鼠GBM,并产生相同的疾病模型。未经验证或推荐用于小鼠。

 

羊抗大鼠肾小球基底膜(gbm)血清(ptx-001agbm)

被动抗gbm肾炎的诱导

 

提供的材料:

抗gbm血清,25ml,0.02m磷酸盐缓冲生理盐水,pH7.3。储存温度在-20摄氏度或更低。产品在56℃下热处理30分钟。避免重复冻融。解冻时可能会形成轻微沉淀,离心转速x 3000-5000转,注射前30分钟。未在小鼠身上验证。

使用说明:

按照以下说明使用时,每瓶含有足够的抗体,可在至少25只大鼠*(175-200 gm)中诱发被动抗BM肾炎。开始手术前请仔细阅读。注射抗gbm血清:麻醉大鼠,在尾静脉注射抗gbm血清15秒。疾病的产生是剂量依赖性的,重要的是提供完整剂量的抗体(推荐剂量为0.4-0.5ml/100gm体重)。由于抗血清批次、大鼠来源和研究者偏好的可变性,我们建议进行剂量-反应研究,从每批抗血清中确定所需的疾病严重程度。肾脏疾病描述:异源性疾病:注射单一抗gbm丸后3-5天内,免疫荧光在肾小球基底膜内呈线状沉积。大鼠c3也呈线性分布,24小时后出现蛋白尿。注射抗gbm抗体8-10天后,大鼠蛋白尿和肾小球定位增加,自体疾病变得明显。

(自体)免疫组织化学呈线性模式的IgG(图1)。到3周时,蛋白尿有望达到200毫克/24小时,肾小球病变(图2)明显,随着时间的推移,严重程度增加,导致肾小球硬化。

 

图1.抗gbm肾炎3周后肾小球毛细血管壁免疫沉积(大鼠igg)的免疫荧光定位。

 

 

图2.抗gbm病3周后肾小球新月体、系膜高细胞性和毛细血管变形。

1Salant DJ,Cybulsky AV:实验性肾小球肾炎。

酶解62:421-4611988。

*建议使用Sprague-Dawley菌株。其他未检测菌株。

#未用probetex抗gbm抗体证实的慢性病。

 

 

 

Probetex PTX-002S:绵羊抗大鼠Fx1A血清
(Heymann肾炎),
用于诱导膜性肾小球肾炎

  • 上皮下免疫复合病
  • 足细胞病
  • 蛋白尿

Sheep Anti-Rat Fx1A Serum (PTX-002S)

For the Induction of Passive Heymann Nephritis (PHN)

提供的材料:

抗FX1A血清,25毫升,0.02米磷酸盐缓冲生理盐水PBS,pH值7.3。储存温度在-20摄氏度或更低。避免反复冻融。使用前离心3000转/分。这种产品还没有在老鼠身上验证过。

使用说明:

该包装含有足够的抗体,当按照以下说明使用时,可诱发25只大鼠(175-200 gm)的被动海曼肾炎。开始手术前请仔细阅读。注射抗fx1a血清:麻醉大鼠,在尾静脉注射0.4ml/100gm体重的抗fx1a血清,持续15-30秒。疾病的产生是剂量依赖性的,重要的是抗体的*剂量的传递。如本数据表所述,测试推荐剂量以产生疾病。由于抗血清批次、大鼠来源和研究者偏好的可变性,我们建议进行剂量-反应研究,从每批抗血清中确定所需的疾病严重程度。

肾脏疾病描述1

异源性疾病注射单剂量抗-x1a后3-5天,肾小球毛细血管内可见异源性绵羊igg弱中度免疫荧光沉积,c3也呈颗粒状分布,5天后出现蛋白尿。

蛋白尿可达到100-200毫克/24小时(布拉德福德分析)。在初的几周内,肾小球在常规光镜下是不明显的,大约3个月后进展为基底膜增厚,系膜基质增加,可能硬化。

 

 

图1a.heymann肾炎发生2周后,肾小球毛细血管壁内呈“串珠状”分布的免疫沉积(绵羊igg)的免疫荧光定位。(b):电子致密沉积位于上皮下间隙。

一。Salant DJ,Cybulsky AV:实验性肾小球肾炎。甲基酶162:421-4611988。

*推荐使用雄性Sprague-Dawley菌株。其他未检测菌株。

#未用probetex抗fx1a抗体证实的慢性病

细胞增殖及毒性检测试剂盒

上海金畔生物科技有限公司提供细胞增殖及毒性检测试剂盒 ,欢迎访问官网了解更多产品信息。

产品编号 BA00208
英文名称 Cell Counting Kit-8
中文名称 细胞增殖及毒性检测试剂盒
别    名 Cell Counting Kit-8 (CCK-8); CCK-8; WST-8; Enhanced Cell Counting Kit-8; CCK-8 Cell Proliferation and Cytotoxicity Assay Kit; CCK-8 WST-8; Cell Counting Kit – 8;   CCK-8细胞增殖及毒性检测试剂盒; CCK8试剂盒; CCK-8/WST-8试剂盒; CCK-8细胞活力检测试剂盒; 细胞计数试剂盒-8(CCK-8); CCK-8试剂盒(细胞增殖及毒性检测试剂盒); Cell Counting Kit-8(CCK-8试剂盒); 细胞计数试剂盒-8; Cell Counting Kit-8(CCK-8)细胞活性检测试剂盒; 细胞活性检测试剂盒;
细胞增殖及毒性检测试剂盒
Specific References  (99)     |     BA00208 has been referenced in 99 publications.
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[IF=10.75] Liu, Zhenni. et al. CD73/NT5E-mediated ubiquitination of AURKA regulates alcohol-related liver fibrosis via modulating hepatic stellate cell senescence. INT J BIOL SCI. 2023 Jan;19(3):950-966  
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[IF=10.684] Peng Luo. et al. OP3-4 peptide sustained-release hydrogel inhibits osteoclast formation and promotes vascularization to promote bone regeneration in a rat femoral defect model. BIOENG TRANSL MED. 2022 Oct;:e10414  Other ;  Other.  
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[IF=9.685] Yang, Wenjing. et al. BTN3A1 promotes tumor progression and radiation resistance in esophageal squamous cell carcinoma by regulating ULK1-mediated autophagy. CELL DEATH DIS. 2022 Nov;13(11):1-17  
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[IF=9.417] Zhenjia Che. et al. Bifunctionalized hydrogels promote angiogenesis and osseointegration at the interface of three-dimensionally printed porous titanium scaffolds. MATER DESIGN. 2022 Nov;223:111118  Other ;  Other.  
PubMed:10.1016/j.matdes.2022.111118

[IF=9.229] Mengjia Wang. et al. Binding Peptide-Promoted Biofunctionalization of Graphene Paper with Hydroxyapatite for Stimulating Osteogenic Differentiation of Mesenchymal Stem Cells. Acs Appl Mater Inter. 2021;XXXX(XXX):XXX-XXX  
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[IF=8.786] Junjun Guo. et al. TIGAR deficiency induces caspase-1-dependent trophoblasts pyroptosis through NLRP3-ASC inflammasome. FRONT IMMUNOL. 2023; 14: 1114620  
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[IF=8.786] Xiaogang Shen. et al. Integrating machine learning and single-cell trajectories to analyze T-cell exhaustion to predict prognosis and immunotherapy in colon cancer patients. FRONT IMMUNOL. 2023; 14: 1162843  
PubMed:37207222

[IF=8.025] Gaofeng Cai. et al. Structure of a Pueraria root polysaccharide and its immunoregulatory activity on T and B lymphocytes, macrophages, and immunosuppressive mice. INT J BIOL MACROMOL. 2023 Jan;:123386  
PubMed:36702224

[IF=8.013] Yuchen Liu. et al. Docosahexaenoic Acid Attenuates Radiation-Induced Myocardial Fibrosis by Inhibiting the p38/ET-1 Pathway in Cardiomyocytes. INT J RADIAT ONCOL. 2022 Dec;:  
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[IF=7.666] Shulipan Mulati. et al. 6-Shogaol Exhibits a Promoting Effect with Tax via Binding HSP60 in Non-Small-Cell Lung Cancer. CELLS-BASEL. 2022 Jan;11(22):3678  
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[IF=7.419] Li-Ping Yu. et al. In vivo identification of the pharmacodynamic ingredients of Polygonum cuspidatum for remedying the mitochondria to alleviate metabolic dysfunction–associated fatty liver disease. BIOMED PHARMACOTHER. 2022 Dec;156:113849  Other ;  Other.  
PubMed:36252355

[IF=7.397] Shunjie Yu. et al. TIM3/CEACAM1 pathway involves in myeloid-derived suppressor cells induced CD8+ T cells exhaustion and bone marrow inflammatory microenvironment in myelodysplastic syndrome. IMMUNOLOGY. 2022 May 03  
PubMed:35470423

[IF=7.109] Zhao, Renchang. et al. Circular RNA circTRPS1-2 inhibits the proliferation and migration of esophageal squamous cell carcinoma by reducing the production of ribosomes. CELL DEATH DISCOV. 2023 Jan;9(1):1-10  
PubMed:36635258

[IF=6.656] Gaofeng Cai. et al. The secretion of sIgA and dendritic cells activation in the intestinal of cyclophosphamide-induced immunosuppressed mice are regulated by Alhagi honey polysaccharides. PHYTOMEDICINE. 2022 Aug;103:154232  
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[IF=6.656] Zhenwei Zhou. et al. BuShen JianGu Fang alleviates cartilage degeneration via regulating multiple genes and signaling pathways to activate NF-κB/Sox9 axis. PHYTOMEDICINE. 2023 May;113:154742  
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[IF=6.58] Luqin Wan. et al. The advanced glycation end-products (AGEs)/ROS/NLRP3 inflammasome axis contributes to delayed diabetic corneal wound healing and nerve regeneration. Int J Biol Sci. 2022; 18(2): 809–825  Other ;  Other.  
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[IF=6.53] Shilei Zhang. et al. A novel PHD2 inhibitor acteoside from Cistanche tubulosa induces skeletal muscle mitophagy to improve cancer-related fatigue. BIOMED PHARMACOTHER. 2022 Jun;150:113004  Other ;  Other.  
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[IF=6.53] Xing-Hua Xiao. et al. Magnolol alleviates hypoxia-induced pulmonary vascular remodeling through inhibition of phenotypic transformation in pulmonary arterial smooth muscle cells. BIOMED PHARMACOTHER. 2022 Jun;150:113060  
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[IF=6.529] Huifeng Wang. et al. Periplocin ameliorates mouse age-related meibomian gland dysfunction through up-regulation of Na/K-ATPase via SRC pathway. Biomed Pharmacother. 2022 Feb;146:112487  
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[IF=6.289] Zhengqing Zhu. et al. Three-dimensionally printed porous biomimetic composite for sustained release of recombinant human bone morphogenetic protein 9 to promote osteointegration. Mater Design. 2021 Oct;208:109882  Other ;  
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保存条件 Protect from light. Store at 2-8℃ for one year. Store at -20℃ for two year.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Cell Counting Kit-8,即增强型 CCK-8 试剂盒,是一种基于 WST-8 而广泛应用于细胞增殖和细胞毒性的快速、高灵敏度、无放射性的比色检测试剂盒。WST-8 在电子耦合试剂存在的情况下,可以被线粒体内的一些脱氢酶还原生成橙黄色的 formazan,且细胞增殖越多越快,则颜色越深;细胞毒性越大,则颜色越浅。对于同样的细胞,颜色的深浅 (生成的 formazan 量) 和细胞数目呈线性关系。

WST-8 是 MTT 的一种升级替代产品,和 MTT 或其它 MTT 类似产品,如 XTT、MTS 等相比有明显的优点。第一,MTT 被线粒体内的一些脱氢酶还原生成的 formazan 不是水溶性的,需要有特定的溶剂来溶解;而 WST-8 和 XTT、MTS 产生的 formazan 都是水溶性的,可以省去后续的溶解步骤。第二,WST-8 产生的 formazan 比 XTT 和 MTS 产生的 formazan 更易溶解。第三,WST-8 比 XTT 和 MTS更加稳定,使实验结果更可靠。第四,WST-8 和 MTT、XTT 等相比线性范围更宽,灵敏度更高,并且更加稳定。

WST-8 对细胞无明显毒性,可以直接加入到细胞样品中,无需预配各种成分;显色后,可以在不同时间反复用酶标仪读板,检测时间更加灵活,便于确定最佳测定时间;酚红和血清对本试剂盒的测定无明显影响。

产品图片
细胞增殖及毒性检测试剂盒
Bioss CCK-8产品(BA00208)与国际G品牌对比图:图中左侧样本为SP2/O的检测数据,右侧样本为3T3的检测数据.Bioss产品稳定性与特异性与国际G品牌同等优质.

细胞增殖及毒性检测试剂盒
Bioss CCK-8产品(BA00208)与国际D品牌对比图:图中左侧样本为SP2/O的检测数据,右侧样本为3T3的检测数据.Bioss产品稳定性与特异性与国际D品牌同等优质.