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Human Kallikrein

HPKa 1303             Human Kallikrein

Activation of Prekallikrein with Factor α-XIIa produces the enzymatically active Kallikrein. Kallikrein is a serine protease which consists of a heavy chain （Mr 52kD） and light chains （Mr either 36 or 33 kD） linked by disulfide bridges. Kallikrein possesses enzymatic activity toward Factor XII, HK, Plasminogen, Factors XI, IX, and VII, prorenin and the complement system. After activation, the activating enzyme Factor XIIa is removed by affinity chromatography. Human Kallikrein purity is determined by SDS-PAGE and shows complete reduction upon incubation with 2-mercaptoethanol.

Buffer composition = 4 mM Sodium Acetate-HCl/0.15 M NaCl/pH 5.3
*Extinction Coefficient （1%） = 11.7
Molecular Weight = ~86,000 daltons