鼠脑切片模具和组织切片模具


鼠脑切片模具和组织切片模具

简要描述:大小鼠脑切片模具
脑模具包括大鼠脑模具和小鼠脑模具。脑模具经过精密机械加工和高度抛光,可确保切片过程的可重复性。使用材料为不锈钢,结实耐用,可被加热、冷却、高压灭菌(只限不锈钢型)、擦洗,经得起苛刻的使用条件。冠状脑模具具有矢状中线,可方便的分离左右脑半球。切片精度精确至1 mm,小鼠脑模具也适用于新生大鼠。

详细介绍

产品咨询

大小鼠脑切片模具
脑模具包括大鼠脑模具和小鼠脑模具。脑模具经过精密机械加工和高度抛光,可确保切片过程的可重复性。使用材料为不锈钢,结实耐用,可被加热、冷却、高压灭菌(只限不锈钢型)、擦洗,经得起苛刻的使用条件。冠状脑模具具有矢状中线,可方便的分离左右脑半球。切片精度精确至1 mm,小鼠脑模具也适用于新生大鼠。
 

Brain and Tissue Matrices

These high quality Brain Matrices are designed for freehand slicing of discrete regions of the brain. They allow slicing of either coronal or sagittal sections through the brain at intervals of 1mm and of 2mm for large brains. The all-metal design with a hard, durable chrome coating offers superior cooling properties. They may be heated, sterilized, chilled and scrubbed clean without damaging the surfaces. All Brain Matrices of the same animal are identical to give reproducible sections.

They are ideal for precise and reproducible blocking of living or fixed brain tissue in:

Anatomy: Precise blocking of the brain prior to microtome sectioning;

Neurophysiology: Reproducible blocking of the brain prior to sectioning; and

Biochemical Pharmacology: Reproducible removal of small brain regions for biochemical analysis. Below are selected product examples linked to ordering information:
 

 

These Matrices may be heated, sterilized, chilled and scrubbed clean without damaging the surfaces. Precise, reproducible blocking of living or fixed brain tissue.
Immediate soaking in liquid cleaner is advisable after use.
To keep tissue cool and viable, chill matrices before use.

go to Blades for use in Brain Matrices

go to Blades for use in Large Brain Matrices

The following are selected product examples that are linked to the ordering information below:

15039
Rhesus Monkey,
Coronal, 2mm
15043
Cynomolgus Monkey,
Coronal, 2mm

15044
Cynomolgus Monkey,
2mm Cerebellum

15041
Cat, Coronal, 2mm
15026
Rabbit, Coronal, 1mm
15022
Guinea Pig, Coronal, 1mm
15001
Gerbil, Coronal, 1mm
15007
Rat, Coronal, 1mm
15006
Rat Sagittal, 1mm
15009
Rat, Dorsal/Ventral, 1mm
15031
Rat, Heart, 1mm
15002
Gerbil, Sagittal, 1mm
15003
Mouse, Coronal, 1mm
15013
Tissue Matrix,
10 x 10mm Chamber,
1mm
15019
6mm Spherical Matrix, 1mm
15017
Tissue Matrix,
10mm rod or V, 1mm

Matrix Ordering Information

 

Mouse
Prod # Description Unit Price Order / Quote
15003
Mouse, 30g adult, Coronal, 1mm
each
$600.00

Qty:
15004
Mouse, 30g adult, Sagittal, 1mm
each
600.00

Qty:

 

Rat
Prod # Description Unit Price Order / Quote
15028
Rat pup, (12-14 days) 30g, Coronal, 1mm
each
$600.00

Qty:
15029
Rat pup, (12-14 days) 30g, Sagittal, 1mm
each
600.00

Qty:
15005
Rat, 125-185g adult, Coronal, 1mm
each
600.00

Qty:
15006
Rat, 125-185g adult, Sagittal, 1mm
each
600.00

Qty:
15007
Rat, 200-400g adult, Coronal, 1mm
each
600.00

Qty:
15008
Rat, 200-400g adult, Sagittal, 1mm
each
600.00

Qty:
15009
Rat, 200-400g adult, Dorsal/Ventral, 1mm
each
673.50

Qty:
15031
Rat, adult, Heart Matrix, 1mm
each
673.50

Qty:

 

Gerbil
Prod # Description Unit Price Order / Quote
15001
Gerbil, 70g adult, Coronal, 1mm
each
$673.50

Qty:
15002
Gerbil, 70g adult, Sagittal, 1mm
each
673.50

Qty:

 

Guinea Pig
Prod # Description Unit Price Order / Quote
15022
Guinea Pig, 350g adult, Coronal, 1mm
each
$673.50

Qty:
15023
Guinea Pig, 350g adult, Sagittal, 1mm
each
673.50

Qty:

 

Ferret
Prod # Description Unit Price Order / Quote
15024
Ferret, adult, Coronal, 1mm
each
$673.50

Qty:
15025
Ferret, adult, Sagittal, 1mm
each
673.50

Qty:

 

Rabbit
Prod # Description Unit Price Order / Quote
15026
Rabbit, 70g adult, Coronal, 1mm
each
$780.00

Qty:
15027
Rabbit, 70g adult, Sagittal, 1mm
each
780.00

Qty:

 

Hamster
Prod # Description Unit Price Order / Quote
15037
Hamster, 100g, Coronal, 1mm
each
$673.50

Qty:
15038
Hamster, 100g, Sagittal, 1mm
each
673.50

Qty:

 

Cat
Prod # Description Unit Price Order / Quote
15041
Cat, 35 slots, Coronal, 2mm
each
P.O.R.

Qty:

 

Dog
Prod # Description Unit Price Order / Quote
15040
Dog, 40 slots, 2mm
each
P.O.R.

Qty:

 

Monkey
Prod # Description Unit Price Order / Quote
15039
Monkey, Rhesus, Adult (2-3 year old, weight 2-3Kg) 40 slots, Coronal, 2mm
each
P.O.R.

Qty:
15043
Monkey, Cynomolgus, adult, Cerebrum, 2mm
each
P.O.R.

Qty:
15044
Monkey, Cynomolgus, adult, Coronal, 2mm
each
P.O.R.

Qty:

 

Tissue Matrix
Prod # Description Unit Price Order / Quote
15013
Tissue Matrix, 10 x 10mm Chamber, 1mm
each
$673.50

Qty:
15014
Tissue Matrix, 15 x 15mm Chamber, 1mm
each
673.50

Qty:
15015
Tissue Matrix, 20 x 20mm Chamber, 1mm
each
673.50

Qty:
15016
Tissue Matrix, 25 x 25mm Chamber, 1mm
each
673.50

Qty:
15017
Tissue Matrix, 10mm rod shape or “V”, 1mm
each
673.50

Qty:
15018
Tissue Matrix, 4mm spherical chamber, 1mm
each
673.50

Qty:
15019
Tissue Matrix, 6mm spherical chamber, 1mm
each
673.50

Qty:

PELCO® Technical Notes, Brain and Tissue Matrices (PDF File)
Blades for Brain Matrices – go to Dissection Tools – Blades
 

 

Stainless Steel Brain Matrices, 1mm

Matrices are precisely machined to insure reproducible sections. This will allow the investigator to slice either coronal (perpendicular to center line) or sagittal (parallel to center line) sections at 1mm intervals.

Features:

  • Stainless Steel
  • Highly polished
  • Precision machined
  • Precise blocking of the brain
  • Repeatable 1mm segments

Size A B C D E Cavity
depth
15065, Small rat, coronal (175 – 300g) 4.76 15.9 12.7 36.6 23.8 7.61
15066, Large rat, coronal (300 – 600g) 4.76 19.8 14.7 36.6 24.7 10.91
15067, Mouse, coronal (40 – 75g) 3.18 11.1 8.73 19.1 12.2 7.4
15068, Small rat, sagittal (175 – 300g) 4.76 15.9 12.7 36.6 23.8 7.61
15069, Large rat, sagittal (300 – 600g) 4.76 19.8 14.7 36.6 24.7 10.91

(all sizes in mm)

Go to Blades for use in Brain Matrices

Prod # Description Unit Price Order / Quote
15065
Rat, 175-300g, coronal, 1mm
each
$324.50

Qty:
15066
Rat, 300-600g, coronal, 1mm
each
324.50

Qty:
15067
Mouse, 40-75g, coronal, 1mm
each
324.50

Qty:
15068
Rat, 175-300g, sagittal, 1mm
each
324.50

Qty:
15069
Rat, 300-600g, sagittal, 1mm
each
324.50

Qty:

Stainless Steel Brain/Heart Matrices, 0.5mm

These Matrices are made of hard stainless steel. They can be cooled, cleaned, and autoclaved. They have smooth surfaces that do not chip. The investigator will be able to get repeatable coronal or sagittal sections enabling precise blocking prior to microtome sectioning.

It is advisable to not let any tissue dry onto the matrix but rather soak the matrix immediay after use for easier cleaning.

Features:

  • Stainless Steel
  • Highly polished
  • Precision machined
  • Precise blocking of the brain or heart
  • Repeatable 0.5mm segments

Size A B C D E Cavity
depth
15045, Mouse, coronal (40 – 75g) 3.18 11.1 8.73 19.1 12.2 7.4
15046, Mouse, sagittal (40 – 75g) 3.18 11.1 8.73 19.1 12.2 7.4
15047, Large Rat, coronal (300 – 600g) 4.76 19.8 14.7 36.6 24.7 10.91
15048, Large Rat, sagittal (300 – 600g) 4.76 19.8 14.7 36.6 24.7 10.91
15056, Mouse, heart 8.3 12.1       4.8
15057, Rat, heart 12.7 19.9       9.6

(all sizes in mm)

Go to Blades for use in Brain Matrices

Prod # Description Unit Price Order / Quote
15045
Mouse, 40 – 75g, coronal, 0.5mm
each
$376.00

Qty:
15046
Mouse, 40 – 75g, sagittal, 0.5mm
each
376.00

Qty:
15047
Rat, 300 – 600g, coronal, 0.5mm
each
376.00

Qty:
15048
Rat, 300 – 600g, sagittal, 0.5mm
each
376.00

Qty:
15056
Mouse heart, 0.5mm
each
376.00

Qty:
15057
Rat heart, 0.5mm
each
376.00

Qty:

Acrylic Brain Matrices

An economical alternative to metal brain matrices. The acrylic coronal brain matrices have the additional feature of a mid-line sagittal cut to facilitate the separation of the left and right hemispheres. All divisions are 1mm apart.

Go to Blades for use in Brain Matrices

Prod # Description Unit Price Order / Quote
15050
Mouse, 30g adult, Coronal, 1mm
each
$165.00

Qty:
15051
Mouse, 30g adult, Sagittal, 1mm
each
165.00

Qty:
15052
Rat, 175-300g adult, Coronal, 1mm
each
165.00

Qty:
15053
Rat, 175-300g adult, Sagittal, 1mm
each
165.00

Qty:
15054
Rat, 300-600g adult, Coronal, 1mm
each
165.00

Qty:
15055
Rat, 300-600g adult, Sagittal, 1mm
each
165.00
 


Tissue-Tek组织包埋盒


Tissue-Tek组织包埋盒

简要描述:Tissue-Tek® Process/Embedding Cassette Made from chemical resistant plastic.

详细介绍

产品咨询

 

包埋耗材, Capsules, 模具, 和记号笔
 
Tissue Tek®; Sakura Finetek包埋模具
1. Tissue-Tek® Process/Embedding Cassette
Made from chemical resistant plastic. These cassettes have an angled, texture writing surface, ensuring positive identification throughout processing, embedding, sectioning and storage. Stainless steel cover is available separay
Cat. No.
Tissue-Tek
标签
Pack
Price
 
62520-C
4191
透明
1500/
3519
Add to Cart
62520-W
4187
白色
1500/
3502
Add to Cart
62520-A
4129
浅绿
1500/
3876
Add to Cart
62520-B
4184
蓝色
1500/
3876
Add to Cart
62520-G
4128
金色
1500/
3910
Add to Cart
62520-GR
4183
绿色
1500/
3910
Add to Cart
62520-O
4126
橙色
1500/
3910
Add to Cart
62520-P
4182
粉红
1500/
3910
Add to Cart
62520-Y
4179
黄色
1500/
3910
Add to Cart
62520-25
4194
SS Cassette Cover
25/
731
Add to Cart
2. Tissue-Tek® Embedding Rings
Made from polystyrene and reinforced with the dual bar center support to prevent warping, these rings have a cross-hatch surface design that provide excellent paraffin bonding for clamping and sectioning.
Cat. No.
Tissue-Tek
Description
Pack
Price
 
62525-W
4151
1000/
3179
Add to Cart
62525-B
4116
1000/
3179
Add to Cart
62525-G
4116
绿
1000/
3179
Add to Cart
62525-P
4116
粉红
1000/
3179
Add to Cart
62525-Y
4116
1000/
3179
Add to Cart
3. Tissue-Tek® 金属包埋模具
Made from high quality stainless steel for optimal thermal conductivity, and they have a well polished surface for easy paraffin block removal. All of the corners are rounded for good paraffin ribboning. These Base Molds are for all applications in specimen embedding with Tissue-Tek® Embedding Rings shown above.
Cat. No.
Tissue-Tek
Description MM
Pack
Price
 
62527-16
4121
模具规格16x16x6
12/
1513
Add to Cart
62527-22
4122
模具规格22x22x6
12/
1513
Add to Cart
62527-32
4123
模具规格32x25x6
12/
1513
Add to Cart
62527-38
4124
模具规格38x25x6
12/
1734
Add to Cart
62528-22
4131
模具规格22x22x12
12/
1734
Add to Cart
62528-32
4132
模具规格32x25x12
12/
1734
Add to Cart
62528-38
4133
模具规格38x25x12
12/
1734
Add to Cart
Tissue-Tek® Embedding Supplies; Sakura Finetek
1. Tissue-Tek® Mold Release, Concentrate
Use this solution to coat base molds to ensure complete separation of embedding paraffin from the mold. The working solution is prepared by adding 5 ml of Concentrate to 95 ml of ethyl alcohol or isopropyl alcohol. Molds are dipped in solution and dried prior to use.
2. Tissue-Tek® Marking Pencil
These pencils are made from soft lead, which is ideal for marking plastic cassettes.
Cat. No.
Tissue-Tek
Description
Pack
Price
 
62530-01
4141
Mold Release
4 oz
300
Add to Cart
62530-12
4141
Mold Release
12/cs
2300
Add to Cart
62531-01
4160
Marking Pencil
12/pk
300
Add to Cart
62531-06
4160
Marking Pencil
6pk/cs
1300
Add to Cart
 
Tissue-Tek® Cryomold®; Sakura Finetek一次性包埋模具
These disposable plastic Cryomolds produce a uniformly shaped flat surface specimen block with tissue freezing medium (O.C.T.) for frozen sectioning, Standard Cryomold fits directly into Cryobar® freezing wells of the Tissue-Tek® II microtome/cryostat. Use a Cryomold® adapter to adapt smaller biopsy and intermediate Cryomold® molds to freezing wells on the Cryobar® unit.
Cat. No.
Tissue-Tek
Description
Pack
Price
 
62534-25
4557
Standard, 25x20x5mm
100/pk
450
Add to Cart
62534-10
4565
Biopsy, 10x10x5mm
100/pk
450
Add to Cart
62534-15
4566
Intermediate 15x15x5mm
100/pk
450
Add to Cart
Tissue-Tek® II Microtome Object Holders; Sakura Finetek
A reusable brass thermal conductive holder with an embossed surface for the Tissue-Tek II microtome/cryostat and for conventional procedures for frozen tissue embedding.
Cat. No.
Tissue-Tek
Description
Pack
Price
 
62538-01
4560
Solid Object Holder (left image)
each
450
Add to Cart
62538-06
4560
Solid Object Holder
6/pk
1683
Add to Cart
Accessories for Tissue-Tek® CRYO3® Microtome/Cryostat Cryo 2000™
1. Cryomold® Specimen Holders
Plastic Cryomold®, round shapes with a tab for easy handling. Available in standard and biopsy configurations. The standard mold measures 22mm (D) x 5mm deep. The Biopsy mold measures 15mm (D) x 5mm deep.
Cat. No.
Tissue-Tek
Description
Pack
Price
 
62532-01
4728
CRYO3 CryoMold, Standard
100/
500
Add to Cart
62532-12
4728
CRYO3 CryoMold, Standard
1200/
3706
Add to Cart
62533-01
4730
CRYO3 Cryomold, Biopsy
100/
500
Add to Cart
62533-12
4730
CRYO3 Cryomold, Biopsy
1200/
3706
Add to Cart
2. Round Object Holders
Machined from solid aluminum with an embossed surface for holding frozen embedding blocks. Measures: 26mm top diameter (without ‘O’ ring), 28.55mm diameter with ‘O’ ring, 23mm body diameter, 16mm height .
62537-01
4726
Round Object Holder, 25mm
each
580
Add to Cart
62537-06
4726
Round Object Holder, 25mm
6/cs
3060
Add to Cart
Autopsy Baskets and Cassettes
Made from stainless steel. It is fully perforated allowing for free movement of fluids. It comes with a snap-cap which provides positive enclosure.
62310-03 is no longer available. Please see 62320-01.
Catalog #
Description
Measures, mm
Pack
Price
 
62310-12
SS Autopsy Cassettes
50(L)x25(W)x12(D)
each
1000
Add to Cart
Embedding Rings
Molded from the highest quality plastic, our Embedding Rings fit most base molds. For added convenience, there are four colors from which to choose (Blue, Pink, Green, Yellow). The large writing surface further eases identification and handling. The base measures 1 5/8″ x 1 1/8″ and the ring measures 1″ x 1″ (25 x 25 mm).
62350-W
Embedding Ring White
250/bx
800
Add to Cart
62350-B
Embedding Ring, Blue
250/bx
800
Add to Cart
62350-P
Embedding Ring, Pink
250/bx
800
Add to Cart
62350-G
Embedding Ring, Green
250/bx
800
Add to Cart
62350-Y
Embedding Ring, Yellow
250/bx
800
Add to Cart
一次性包埋盒
Our Disposable Base Mold is designed to fit all existing cassettes. Because our base molds are disposable you save time on cleaning and storing and they offer you less exposure to hazardous chemicals.
62352-07
Disposable Base Mold 7x7x5mm
500/
1400
Add to Cart
62352-15
Disposable Base Mold 15x15x5mm
500/
1400
Add to Cart
62352-24
Disposable Base Mold 24x24x5mm
500/
1400
Add to Cart
62352-30
Disposable Base Mold 30x24x5mm
500/
1400
Add to Cart
62352-37
Disposable Base Mold 37x24x5mm
500/
1400
Add to Cart
Paraffin Tissue Microarrays
Paraffin tissue microarrays (TMAs) are becoming more common in the laboratories. Current techniques including organizing, punching, and inserting tissues is time consuming and involving costly equipments.This Arraymold is the simple tool but great answer for this current technique for laboratories to perform TMAs with less time consuming, excellent results.
With this Arraymold, you can process up to 150 specimens onto one single slide with very little time consuming, minimal technical training.
The DVD instruction is accompanying with each kit, will step by step guide you to perform through the entire TMAs process. Arraymold features include:
  • Allow pathologist or researcher to view many different samples on the same single slide
  • Helpful in finding IHC markers for control or quality assurance from hundreds of samples
  • Staining markers will be more consistent when hundreds of samples are on one slide
  • Used less costly antibody
  • Arraymold kit is small enough to be stored in a drawer
  • Rubber arraymold can be reused many times without losing its flexibility.
Ordering:
Arraymold Kit A, (60 cores)
This mold generates 2 mm diameter sample core, 60 cores per mold. Kit consists of:
10 Disposable 2 mm sterile dermal needle
2 Stylets (used to remove sample from needle)
1 Brass Plate (for setting cryoarray punches)
1 2 mm reusable rubber Arraymold (60 cores)
1 DVD instruction (NTSC) for showing how to use the mold.
69130-10
Arraymold Kit A, 60 Cores
each
16983
Add to Cart
Arraymold Kit B, (150 cores)
This mold generates 1.5 mm diameter sample core, 150 cores per mold. Kit consists of
10 Disposable 2 mm sterile dermal needle
2 Stylets (used to remove sample from needle)
1 Brass Plate (for setting cryoarray punches)
1 1.5 mm reusable rubber Arraymold (150 cores)
1 DVD instruction (NTSC) for showing how to use the mold.
69130-20
Arraymold Kit B, 150 Cores
each
16983
Add to Cart
Arraymold Kit C, (150 cores)
This mold generates 4 mm diameter sample core, 15 cores per mold. Kit consists of:
10 Disposable 4 mm sterile dermal needle
2 Stylets (used to remove sample from needle)
1 Brass Plate (for setting cryoarray punches)
1 4 mm reusable rubber Arraymold (150 cores)
1 DVD instruction (NTSC) for showing how to use the mold.
69130-40
Arraymold Kit C, 15 Cores
each
16983
Add to Cart
Arraymold Kit D, (36 cores)
This mold generates 2 mm diameter sample core, 36 cores per mold. Kit consists of:
10 Disposable 2 mm sterile dermal needle
2 Stylets (used to remove sample from needle)
1 Brass Plate (for setting cryoarray punches)
1 2 mm reusable rubber Arraymold (36 cores)
1 DVD instruction (NTSC) for showing how to use the mold.
69130-15
Arraymold Kit D, 36 Cores
each
11883
Add to Cart
Arraymold Kit E, (72 cores)
This mold generates 1.5 mm diameter sample core, 72 cores per mold. Kit consists of:
10 Disposable 1.5 mm sterile dermal needle
2 Stylets (used to remove sample from needle)
1 Brass Plate (for setting cryoarray punches)
1 1.5 mm reusable rubber Arraymold (72 cores)
1 DVD instruction (NTSC) for showing how to use the mold.
69130-25
Arraymold Kit E, 72 Cores
each
11883
Add to Cart
Arraymold Kit F, (35 cores)
This mold generates 3 mm diameter sample core, 35 cores per mold. Kit consists of:
10 Disposable 3 mm sterile dermal needle
2 Stylets (used to remove sample from needle)
1 Brass Plate (for setting cryoarray punches)
1 4 mm reusable rubber Arraymold (35 cores)
1 DVD instruction (NTSC) for showing how to use the mold.
69130-45
Arraymold Kit F, 35 Cores
each
16983
Add to Cart
Arraymold Kit G, (24 cores)
This mold generates 3.5 mm diameter sample core, 24 cores per mold. Kit consists of:
10 Disposable 3.5 mm sterile dermal needle
2 Stylets (used to remove sample from needle)
1 Brass Plate (for setting cryoarray punches)
1 4 mm reusable rubber Arraymold (24 cores)
1 DVD instruction (NTSC) for showing how to use the mold.
69130-55
Arraymold Kit G, 24 Cores
each
16983
Add to Cart
Arraymold Package Kit
To save money, you can order Arraymold Package Kit. This package includes two or three Arraymold kits listed above.
  • Arraymold Kit 1: Including Arraymold Kit A and Arraymold Kit B
  • Arraymold Kit 2: Including Arraymold Kit A and Arraymold Kit C
  • Arraymold Kit 3: Including Arraymold Kit B and Arraymold Kit C
  • Arraymold Kit 4: Including all three Arraymold Kit A, B, and C
69131-01
Arraymold Package Kit 1 (A +B)
each
30566
Add to Cart
69131-02
Arraymold Package Kit 2 (A +C)
each
30566
Add to Cart
69131-03
Arraymold Package Kit 2 (B +C)
each
30566
Add to Cart
69131-04
Arraymold Package Kit 2 (A +B+C)
each
45509
Add to Cart
Spare Accessories for the ArrayMold
69130-56
Punch Needle 1.5mm
50/pk
3315
Add to Cart
69130-57
Punch Needle 2.0mm
50/pk
3315
Add to Cart
69130-58
Punch Needle 3.0mm
50/pk
3315
Add to Cart
69130-59
Punch Needle 3.5mm
50/pk
3315
Add to Cart
69130-62
Punch Needle 4.0mm
50/pk
3315
Add to Cart
69130-63
Styler 1.5mm
each
500
Add to Cart
69130-64
Styler 2.0mm
each
500
Add to Cart
69130-65
Styler 3.0mm
each
500
Add to Cart
69130-66
Styler 3.5mm
each
500
Add to Cart
69130-67
Styler 4.0mm
each
500
Add to Cart
Micro Baskets
Made from stainless steel and has a positive clip-on cover. It is ideal for processing micro specimens. It can be used as a holder for paraffin sections while removing paraffin wax. Available in medium and fine mesh. It measures: 20mm(dia.)x 5mm(deep).
62312-10
Micro Baskets, Medium Mesh
each
480
Add to Cart
62312-16
Micro Baskets, Medium Mesh
6/pk
1904
Add to Cart
62312-20
Micro Baskets, Fine Mesh
each
480
Add to Cart
62312-26
Micro Baskets, Fine Mesh
6/pk
1904
Add to Cart
Expandable Capsules
Made from stainless steel and comes with a positive snap cap. They are expandable. When closed its dimensions are 40mm(dia.)x 7mm (deep) and can be expanded to 11mm deep. Perforated top and bottom.
62314-01
Expandable Capsule
each
580
Add to Cart
62314-06
Expandable Capsule
6/pk
2720
Add to Cart
Multi-Purpose Capsules
Product 62316 no longer is available. Please see 62320.
Stainless Steel Tissue Capsules
Features:
  • A secure positive snap-on cover.
  • Perforated on the top and bottom for easy fluid circulation.
  • Can be partitioned for up to 4 sections.
The separator is not included
They measure: 37mm(I.D.) x 6mm(deep) (17/16″ x 3/16″)
62320-01
SS Tissue Capsule
each
450
Add to Cart
62320-06
SS Tissue Capsule
6/pk
1640
Add to Cart
62320-20
Tissue Capsule Separator
2/pk
450
Add to Cart
Plastic Tissue Capsules
Features:
  • Molded from polyethylene; resistant to all processing chemicals, including Zenker’s fixative.
  • A three-compartment divider which is removable for specimens up to 35mm.
  • A weighted end floats the capsule in a upright position to conserve space.
  • Perforated on the top and bottom for better circulation of the fluid.
  • Snap-on hinged cover.
They measure: 35mm(I.D.)x35mm(I.D.)x10mm(deep) (13/8×5/16)
62322-12
Plastic Tissue Capsules
12/pk
1400.00
Add to Cart
Specimen Discs
Accuray machined chrome plated aluminum discs that fit most microtomes and cryostats. They have a knurled surface for better specimen handling. Straight shank. Available in a variety of disc sizes.
62355-01
Specimen Disc, size 7/8″ (2.2cm)
each
1200
Add to Cart
62355-04
Specimen Disc, size 17/8″ (2.9cm)
each
1200
Add to Cart
62355-07
Specimen Disc, size 1½” (3.8cm)
each
1200
Add to Cart
62355-10
Specimen Disc, size 2″ (5.1cm)
each
1200
Add to Cart
Tissue Processing Capsules
These one piece plastic capsules are hinged and perfectly aligned, offering convenience and ease of use. Both the top and the bottom of the capsule have an open mesh offering maximum fluid flow during processing. A frosted marking surface assures permanent identification. These capsules are commonly used with the Embedding Ring System. They are available in two sizes and five colors.
To Use: Simply place the tissue in the capsule, close the cover to lock, and the sample is now ready for processing.
  • The 38mm(Dia.)x8mm(Deep) are intended for use with normal tissue.
  • The 29mm(Dia.)x6mm(Deep) are intended for use with Biopsy tissue
Cat. No
Color
Capsule Size
Pack
Price
 
62358-W
White
38mmx8mm
500
1785
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62359-W
White
29mmx6mm
500
1785
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Embryo Dishes
These dishes are molded from clear glass and come with a glass lid for the viewing or staining of free-floating specimens.
70543-30
Embryo Dish, 30 mm Dia x 12mm Deep
each
350
Add to Cart
70543-45
Embryo Dish, 45 mm Dia x 12mm Deep
each
450
Add to Cart
Paper Biopsy Bags
Due to the amazing demand from of our customers, EMS is re-introducing the original Paper Biopsy Bags. These bags are made from porous paper which provides maximum tissue safety during processing. The bags permit proper fluid exchange and drainage.
To Use: Simply introduce the specimen into the bag and fold its end, enclosing the cassette or capsule – you are now ready to process as usual.
Bag measurement: Overall 13⁄4” W x 3” L (45 x 75 mm), Inside Seam: 13⁄8” W (35 mm)
62326-01
Biopsy Paper Bags
100/pk
350
Add to Cart
62326-05
Biopsy Paper Bags
500/pk
800
Add to Cart
Biopsy Foam Pads
A cellular polyester blue foam that has uniform air pockets throughout the pad for optimal fluid flow. It prevents either small or fragmented tissue specimens from being lost.
To Use: Place small samples or specimen fragments between the two pads and place the specimen in a cassette or capsule.
Cat. No.
Foam Pad Size
Use With
Pack
Price
 
62325-01
25mm Diameter
29mm Capsules
1000/pk
600
Add to Cart
62325-04
35mm Diameter
38mm Capsules
1000/pk
700
Add to Cart
62325-06
25mmx32mm
1×1¼”Cassettes
1000/pk
600
Add to Cart
Biopsy Bags
  • Made from nylon cloth, 0.2mm mesh diameter. All seams are heat-sealed.
  • Allows for unrestricted fluid movement around the tissue.
  • Reduces the loss of small biopsy specimens.
  • Reduces cross contamination and pressure artifacts by using sponges.
62324-35
Nylon Biopsy Bag 35 x 50mm
100/pk
900
Add to Cart
62324-75
Nylon Biopsy Bag 45 x 74mm
100/pk
1200
Add to Cart
62324-98
Nylon Biopsy Bag 98 x 75mm
100/pk
1300
Add to Cart
 
 
 
CellSafe™ Biopsy Insert
The CellSafe is a disposable plastic biopsy insert consisting of two hinged interlocking frames with an integral nylon mesh. The mesh is extra fine and therefore ensures a high level of specimen security.
Measurements:
Overall: 28 mm x 25 mm x 5 mm high (1.1” x 1” x ¼”);
The mesh area: 18 mm x 18 mm x 2 mm high.
62327-10
CellSafe™ Biopsy Insert
100/pk.
1250
Add to Cart
Stainless Steel Biopsy Inserts
A two-part loose fitting stainless steel capsule with welded mesh that fits inside the processing cassettes preventing the loss of small or friable material while allowing interchange of fluids.
They fit all process/embedding cassettes such as 70025 and 72025 as well as Uni-Cassettes (62500).
62328-01
Stainless Steel Biopsy Insert
each
340
Add to Cart
 
Peel Away 可撕性一次性包埋盒
For plastic or paraffin embedding; no block trimming needed. Inside fingers hold ID slip which becomes embedded in the block for positive permanent identification.
70180
Truncated Mold, size 8x8mm
288/cs
2754
Add to Cart
70181
Truncated Mold, size 12x12mm
288/cs
2754
Add to Cart
70182
Square Mold, size 22x22mm
288/cs
2754
Add to Cart
70183
Rectangular Mold, size 22x30mm
288/cs
2754
Add to Cart
70184
Rectangular Mold, size 22x40mm
288/cs
2754
Add to Cart
Adjustable Embedding Mold
These two “L’s” shape and stainless steel base plate form an adjustable embedding mold, suitable for embedding various size specimens with a minimum of waste material. Available in 5 different sizes.
62361-01
Adjustable Mold, 7⁄8″ x 13⁄4″ x ½” (22x44x13mm)
each
1200
Add to Cart
62361-03
Adjustable Mold, 7⁄8″ x 13⁄4″ x 3⁄4″ (22x44x19mm)
each
1200
Add to Cart
62361-05
Adjustable Mold, 7⁄8″ x 13⁄4″ x 1″ (22x44x25mm)
each
1200
Add to Cart
62361-07
Adjustable Mold, 7⁄8″ x 13⁄4″ x 11⁄4″ (22x44x32mm)
each
1400
Add to Cart
62361-09
Adjustable Mold, 3½” x 53⁄4″ x 1″ (89x146x25mm)
each
1500
Add to Cart
Slide Coating Adhesive
Prepared from 3-aminopropyltriethoxysilane in acetone. Good for coating glass slides to adhere paraffin sections for further processing without sections falling off from the slide. READY TO USE.
71302
Slide Coating Adhesive
100 ml
650
Add to Cart
Embedding Blocks
  • Precisely machined from hard phenolic resin .
  • The faces of the embedding blocks are cut very deeply providing a firm hold of the embedded specimen.
  • Suitable for cellodin or paraffin sections.
    Available in four sizes.
62363-01
Embedding Block,
19x19x19mm High
each
900
Add to Cart
62363-02
Embedding Block,
25x25x19mm High
each
1000
Add to Cart
62363-03
Embedding Block,
38x38x19mm High
each
1100
Add to Cart
62363-04
Embedding Block,
51x51x19mm High
each
1300
Add to Cart
Embedding Molds and Mold Release Agents
For a complete selection, please see Molds and Mold Release Agent
Liquid Blocker–Super PAP Pen
The Newest Staining Guard Pen
New and improved. The Super PAP Pen outperforms the original PAP Pen. The Super PAP Pen may be used at temperatures as high as 120°F. (The old style PAP Pen worked only up to 90°F). With this high temperature feature, one can now use the Super PAP Pen to perform the peroxidase-anti-peroxidase method, ABC method, and Fluorescent antibodies method with satisfactory results.
71310
Super PAP Pen, Large
each
1000
Add to Cart
71312
Super PAP Pen, Small
each
820
Add to Cart
 
Aqua-Hold 免疫组化笔
The Aqua-Hold Pap Pen draws a liquid repellent barrier around a section of tissue. Used like a paint pen, you just draw a well on a slide. The barrier creates the proper surface tension to hold aqueous solutions such as costly antibody and other valuable specimens. After application, the barrier material is insoluble in alcohol and acetone but can be removed after staining with xylene. Each pen holds 13ml of long-lasting material, making hundreds of wells.
71311
Aqua-Hold Pen
each
800
Add to Cart
Fro-Marker
A wide tipped pen designed for use with frozen sections. The pen provides a sticky membrane which when applied to the slide and onto which the section is placed it eliminates the section from falling off, moving, or wrinkling on the slide during immunostaining procedures. The membrane is stable up to 110°C and is suitable for vigorous applications such as in-situ hybridization.
71313-10
Fro-Marker
each
1400
Add to Cart
Para-Marker
A wide tipped pen, designed for use with paraffin sections. The pen provides a sticky membrane to the glass slide which not only prevents paraffin sections from falling off but also keeps the sections flat on the slide.
71313-20
Para-Marker
each
1300
Add to Cart
Tissue Capture Pen
With one swift application of the EMS Tissue Capture Pen, you are able to change your plain glass slide into a coated one.
The EMS Tissue Capture Pen is a coating pen with a wide point (18mm) for fixing various tissue sections (paraffin embedded, frozen and formalin sections, etc.) The unique pen is unaffected by heat up to 120ºC so it offers secure and firm adhesion of the tissue section. It may be used in Microwave staining, immunostaining, in situ etc….
One application is all that is needed, and after it is applied, allow to dry for 1-2 minutes.
The EMS Tissue Capture may be used in conjunction with our Pap-Pen ( 71310) and will allow for easy placement of adherent as well as nonadherent cells on a glass slide. Immunostaining/ISH can be performed by applying a few drops of tissue suspension or liquid media onto the glass slide which is circled by the Pap-Pen, and then your section can be placed.
Ordering
71314-10
EMS Tissue Capture Pen
each
1800
Add to Cart
 

细胞 /组织收集保存试剂盒

一、细胞 /组织收集保存试剂盒

1Collection and Transport Kit, Small 

CTK10-S 适用于常规组织及细胞的收集、运输及保存,CTK10-S1适用于淋巴、血样或骨髓等组织样品的收集、运输及保存。样品体积zui大为120 mL. 试剂盒内含:

1125ml无菌收集盒,50/15ml锥形管或血样采集管(用于血样等液体样品)

2)无菌转移液100ml*1: Cat. #CTK10-SZTM:A2™ (Cat. #CTK20-M,含克林霉素,庆大霉素及两性霉素) Cat. #CTK10-S1ZTM:A1™ (Cat. #CTK20-M1,含克林霉素,庆大霉素)

3)无菌塑料组织转运袋

4)各式标签及冰袋

2Collection and Transport Kit, Medium 

Medium kits CTK20-M适用于常规组织及细胞的收集、运输及保存,CTK20-M1适用于淋巴、血样或骨髓等组织样品的收集、运输及保存。样品体积zui大为500ml

试剂盒内含:

11L无菌收集盒或管(用于血样等液体样品)

2)无菌转移液100ml*2: CTK20-MZTM:A2™ (Cat. #CTK20-M,含克林霉素,庆大霉素及两性霉素) CTK20-M1ZTM:A1™ (Cat. #CTK20-M1,含克林霉素,庆大霉素)

3)无菌塑料组织转运袋

4)各式标签及冰袋

 

3Large Cell and Tissue Collection Kits

CTK30-L适用于常规组织及细胞的收集、运输及保存,CTK30-L1适用于淋巴、血样或骨髓等组织样品的收集、运输及保存。样品体积zui大为4.5 L

试剂盒内含:

14.5L无菌收集盒或管(用于血样等液体样品)

2)无菌转移液500ml*1: Cat. #CTK30-LZTM:A2™ (Cat. #CTK20-M,含克林霉素,庆大霉素及两性霉素) Cat. #CTK30-L1ZTM:A1™ (Cat. #CTK20-M1,含克林霉素,庆大霉素)

3)无菌塑料组织转运袋

4)各式标签及冰袋

 

4X-Large Cell and Tissue Collection Kits

CTK40-X适用于常规组织及细胞的收集、运输及保存, CTK40-X1适用于淋巴、血样或骨髓等组织样品的收集、运输及保存。样品体积zui大为8 L

试剂盒内含:

18L无菌收集盒或管(用于血样等液体样品)

2)无菌转移液500ml*2: CTK40-XZTM:A2™ (Cat. #CTK20-M,含克林霉素,庆大霉素及两性霉素) CTK40-X1ZTM:A1™ (Cat. #CTK20-M1,含克林霉素,庆大霉素)

3)无菌塑料组织转运袋

4)各式标签及冰袋

                        

5、血样/体液收集保存试剂盒

? CTK21-MB于血样或液体样本的收集、保存于转运,zui大体积可达1.5 L.

试剂盒内含无菌血样收集盒//袋,保护泡沫,生物冰袋等。

 

二、细胞培养液

1M3基础培养液

?    M3无血清、高营养的拥有产品的基础培养液,适用于多种人及哺乳动物细胞培养, 含多种生长因子、添加剂及优良的缓冲体系,可根据培养细胞的类型添加所需的血清等营养因子,根据是否含有抗生素及酚红指示剂,M3基础培养液可分为以下型号:

培养液

货号

抗生素

酚红

M3:BaseA

M300A

M3:BaseAC

M300AC

M3:BaseF

M300F

M3:BaseFC

M300FC

M3:10A

M310a

M3:10AC

M310AC

M3:D

M3DEF

M3:DC

M3DEC

SMX

MSMXS

M3base 基础培养液中,A型为含复合抗生素,适宜于有污染可能的细胞培养,但是对细胞有一定的伤害,F型不含抗生素;C型不含有酚红指示剂,适用于细胞标记实验,且酚红对部分细胞有生长影响及毒害作用。

M310M3基础培养液添加10%胎牛血清,适宜于胚胎或成体干细胞培养、培养条件严格的间质细胞培养,贴壁细胞培养、多数肿瘤细胞的起始培养液,以及用于促进细胞株生长、分化的培养液等。

M3:D为培养液成分明确,不含任何不明成分生长因子及血清成分,可作为添加特定生长成分实验的对照实验培养液。

SMX为成分明确的生长因子复合物。

储存条件2-8度避光保存,勿冰冻。

不同组织来源细胞分离培养的培养液选择

动物组织

物种

细胞类型及培养液选择

脂肪

大小鼠,仓鼠,兔

间充质干细胞,脂肪血管基质再生干细胞10%血清M3培养液;脂肪细胞(30%血清M3培养液)

骨髓

大小鼠,仓鼠,兔

造血干细胞,间充质干细胞,多种更新前体细胞,内皮细胞20%血清M3培养液)

脑、脊髓、神经组织

大小鼠

前体细胞及分化(5%血清或10%血清的M3培养液)

肠道

大小鼠,仓鼠

原代上皮细胞/间质支持细胞(2%10%血清M3培养液)

大小鼠,仓鼠

原代上皮细胞/间质支持细胞(2%5%10%血清M3培养液)

大小鼠,仓鼠

原代上皮细胞/间质支持细胞(2%5%10%血清M3培养液)

肌肉(外周、心肌、平滑肌)

大小鼠,仓鼠,兔

周细胞,间充质细胞,基质干细胞,可再生细胞(10%血清M3培养液)

胰腺及其它神经内分泌器官

大小鼠,仓鼠

胰岛beta细胞,胰泡细胞(5%血清M3培养液)

血液

大小鼠,仓鼠,兔

间质细胞,内皮细胞(20%血清M3培养液)

皮肤

 

表皮角质形成细胞,成纤维细胞,间质细胞(10%血清M3培养液)

肿瘤等

大小鼠,仓鼠

上皮细胞,间质细胞,淋巴细胞(10%血清M3培养液)

 

2ACE™ 无血清培养液

ACE™是成分明确的,低蛋白 (<20mg/L)的无血清培养液,适宜于适应能力低的细胞培养,以获得良好的生存和生长能力。培养中可不需要二氧化碳, 减少了疫苗生产下游处理程序。同时不添加酚红,在杂交瘤细胞培养时比同类培养液产生更多的抗体。

 

三、冻存液及细胞处理液

1CPZ™ 冻存培养液

?    CPZ™冻存液含有临床级别的淀粉、右旋糖苷及配方(生长因子,%10胎牛血清,冷冻保存剂)。适宜于包括神经细胞在内的多种人及哺乳动物细胞冷冻保存。使用前添加DMSO至终浓度5% to 10% (v/v),(根据冻存细胞类型确定浓度)。冻存细胞(含少量培养液)体积应少于冻存液体积的20%,细胞数通常为1 -25 x 106 cells/mL。将细胞轻柔重悬于冻存液后置于-80度冰箱至少12小时(放于程序冻存盒内使其温度以1/分钟缓慢下降),然后转入–135度冰箱或液氮罐长期保存。

2EZ-CPZ™ 冻存液

?EZ-CPZ™是一种无血清、无蛋白的冻存液,不含任何不明确的或动物来源的成分,适宜于临床或特殊使用目的的细胞冻存(如无血清培养细胞)。冻存于该冻存液的人或其他动物细胞复苏后保持良好的活性、生长性及分化潜能。可以与细胞悬液以11混匀冻存,细胞无需离心。使用前添加DMSO至终浓度5%

 

3ZSol™生物处理液

?ZSol™ 系列生物处理缓冲液无菌无酚红,所有成分均为美国药典级。该系列处理也包括ZSol:M™, ZSol:F™ ZSol:MH™。可用于来自人及动物的细胞、组织处理,也可用于疫苗、病毒、细菌等微生物及真核细胞源产品的生产。包括细胞分离、、基于正常或基因工程细胞的生物制品(蛋白、激素等)、制药、诊断以及科研。

ZSol:M™ ZSol:F™处理液适用于2°C-50°C范围的生物处理,ZSol:MH™处理液适用于低于37度条件下的生物处理。该处理液不能用于细胞冻存的目的。

 

4ZTM™ 转运液

ZTM™转运液无菌,无酚红,化学成分明确,适宜于新鲜分离的组织或细胞的转运或临床细胞运输使用,含有可选择的抗生素及谷氨酰胺衍生物以支持活组织或细胞在运输中或短期保存时的活性,不含人或动物源成分。其中ZTM:A1™ 含有6.5 µg/mL克林达霉素和50 µg/mL庆大霉素, ZTM:A2™含有更多抗生素,包括13 µg/mL克林达霉素、100 µg/mL庆大霉素2.5 µg/mL两性霉素B.

 

5ZAPR™ 红细胞裂解液

?ZAPR™ 系列红细胞裂解液无菌,无酚红,所有成分均为美国药典级别,

 

四、细胞株

1NCM356细胞株(Normal-Derived Colon Mucosa Cell Line,正常的结肠粘膜细胞株):

   来源于一名65岁男性黑人直肠腺癌患者的结肠粘膜上皮,未注入任何外源基因,表达细胞角蛋白、绒毛蛋白结肠上皮细胞抗原,不表达其它特定细胞株表面抗原。部分细胞表达黏液素合成酶及肿瘤相关抗原。不能在软琼脂上生长,无致瘤性。要求严格的生长条件,须在INCELL's M3:10TM 培养液上做长期培养,倍增时间32-35小时。可广泛用于肠道细胞的研究,如消化道感染疾病、细胞信号转导,细胞因子表达,维生素转运及基因调控。

 

2NCM460细胞株(Normal-Derived Colon Mucosa Cell Line,正常的结肠粘膜细胞株)

来源于一名68岁正常西班牙男性的结肠粘膜上皮,未注入任何外源基因,表达细胞角蛋白、绒毛蛋白结肠上皮细胞抗原,不表达其它特定细胞株表面抗原。部分细胞表达黏液素合成酶及肿瘤相关抗原。不能在软琼脂上生长,无致瘤性。要求严格的生长条件,须在INCELL's M3:10TM 培养液上做长期培养,倍增时间32-38小时。可广泛用于肠道细胞的研究,如消化道感染疾病、细胞信号转导,细胞因子表达,维生素转运,基因调控,蛋白表达,多种生长调控因子的磷酸化研究。

 

3IBHK-4细胞株A Baby Hamster Kidney Derived Cell Line,幼仓鼠肾脏细胞)

   IBHK-4细胞株来源于仓鼠肾脏,常规于INCELLACE™培养基上悬浮生长,培养液需要无血清、无抗生素及动物源成分,培养时需要轻微摇晃。能在软琼脂上快速形成集落,因此有致瘤性。该细胞株可广泛应用于病毒培养,用于灭毒疫苗生产。

haemtech 重组组织因子说明书

haemtech 重组组织因子说明书

Recombinant Tissue Factor

重组组织因子


  • 价格:请询价

    尺寸 10 微克、100 微克
    公式 20 mM Tris、150 mM NaCl、pH 8.0、10 mM CHAPS
    贮存 -80°C
    纯度 > 95% 通过 SDS-PAGE;不是组织/细胞培养级。未检测内毒素。
    活动测定 荧光检测
    保质期(正确储存) 12个月
  • 价格:请询价
    尺寸 10 微克、100 微克
    公式 20 mM Tris、150 mM NaCl、10 mM CHAPS pH 7.4
    贮存 -80℃
    纯度 > 95% 通过 SDS-PAGE;不是组织/细胞培养级。未检测内毒素。
    活动测定 荧光检测
    保质期(正确储存) 1年

凝胶 Novex 4-12% Bis-Tris
加载 rTF,每条泳道 1 µg
缓冲 拖把
标准

SeeBluePlus 2; 肌球蛋白 (191 kDa)、磷酸化酶 B (97 kDa)、BSA (64 kDa)、谷氨酸脱氢酶 (51 kDa)、乙醇脱氢酶 (39 kDa)、碳酸酐酶 (28 kDa)、肌红蛋白红 (19 kDa)、溶菌酶 (14) kDa)

本土化从 SF9 细胞中纯化分子量35,000消光系数

1%
1 厘米,280 纳米
= 12.6

动物组织淋巴细胞分离试剂盒(人、猴、羊、猪、鱼)

上海金畔生物科技有限公司提供动物组织淋巴细胞分离试剂盒(人、猴、羊、猪、鱼) ,欢迎访问官网了解更多产品信息。

产品编号 CS121
英文名称 Lymphocyte Separation Medium Kit (Animal tissue)
中文名称 动物组织淋巴细胞分离试剂盒(人、猴、羊、猪、鱼)
别    名 Animal tissue Lymphocyte Separation Medium Kit;   
保存条件 室温避光保存,2年有效
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 试剂盒组成:
各种动物脏器组织淋巴细胞分离液:  200mL
全血及组织稀释液:      200mL
细胞洗涤液:        200mL
产品简介:
本产品是一种用于分离各种动物脏器组织淋巴细胞的无菌、低内毒素水平的密度梯度分离液。适用于从各种动物脏器组织中分离单个核细胞(主要为淋巴细胞),无菌条件下所分离的细胞可用于免疫学检测。其分离原理是根据细胞的密度差异将葡聚糖(右旋糖酐)和泛影酸钠按一定比例混合,调整比重、pH 值和渗透压,经过澄清及过滤除菌后制成一种密度梯度分离液,经过密度梯度离心使一定密度的细胞按相应密度梯度分布,从而将各种细胞加以分离。通过离心使一定密度的细胞按相应密度梯度分布,从而将淋巴细胞从各种动物脏器组织中分离出来。
产品参数:
密度:1.077±0.001g/mL
渗透压:290~350 mOsm/kg H2O
无菌:0.1μm 滤膜过滤
适用动物种属:人、猴、羊、猪、鱼的组织(脾脏、骨髓、脏器组织、肿瘤浸润组织)淋巴细胞分离及单个核细胞分离。
组织单细胞悬液制备方法(仅供参考):
组织研磨法:
1. 无菌条件下摘取脏器组织,撕去被膜,用眼科剪将脏器组织剪成小块。
2. 将尼龙筛网或者是细胞过滤筛放置于平皿上,加入少量全血及组织稀释液(保证脏器组织及获得的细胞处于液体环境中)。
3. 将脏器组织放置于筛网上,使用注射器活塞或者是无菌镊子来研磨脏器组织(尽量控制研磨力度,保持筛网悬空,避免在皿底上直接研磨而造成大批细胞死亡)。
4. 研磨完全后使用全血及组织稀释液冲洗筛网,收集细胞悬液,再经滤网过滤。
注:
1. 可用酶消化法,使用胶原酶对脏器组织组织进行消化,得到单细胞悬液。
2. 如果最终得到的细胞需要培养,那全过程所需试剂与器材均要求无菌。
3. 根据脏器组织的体积控制单细胞悬液的浓度在108~9个/mL。
淋巴细胞分离方法:
1. 制备脏器组织单细胞悬液。
2. 取一支适当的离心管,加入与组织单细胞悬液等量的分离液(分离液最少不得少于3 mL,总体积不能超过离心管的三分之二,否则会影响分离效果)。
3. 小心吸取单细胞悬液加于分离液液面上,注意保持两液面界面清晰。(可以使用巴氏德吸管吸 取单细胞悬液,然后小心的平铺于分离液上,因为两者的密度差异,将形成明显的分层界面。)
4. 室温,水平转子500~900 g,离心20~30 min。(根据脏器组织单细胞悬液的量确定离心条件,单细胞悬液量越大,离心力越大,离心时间越长,具体离心条件可以自行摸索,以达到最佳分离效果)。
5. 离心后,此时离心管中由上至下细胞分四层。第一层为稀释液层;第二层为环状乳白色淋巴细胞层;第三层为透明分离液层;第四层为红细胞层。
6. 用吸管小心吸取第二层环状乳白色淋巴细胞层至另一洁净的 15 mL离心管中,向离心管中加入10ml细胞洗涤液洗涤白膜层细胞,250 g,离心10 min。
7. 弃上清,5 mL的PBS或细胞清洗液重悬细胞,250 g,离心10 min。
8. 重复步骤7
9. 弃上清,细胞重悬备用。
注意事项:
1. 开封前颠倒混匀,本分离液为无菌产品,为延长分离液保存时间,请在无菌条件下启封,避免污染。
2. 待分离的组织要求新鲜,避免冷冻和冷藏。
3. 本实验要求,在正常大气压下,分离液、分离样本以及分离环境温度为(18℃~25℃)。分离液在低温时呈较高密度,在高温时呈较低密度。细胞分离液从冰箱取出后,不可立即使用,操作前可将样本和分离液置于20℃水浴中复温20分钟以保证分离温度。4℃或者是温度较低的条件下离心,可能会导致白膜层中红细胞污染加重。
4. 稀释血液或洗涤细胞,不可使用含Ca、Mg离子的缓冲液及培养液,其成分会导致血细胞凝集,大大降低细胞得率及纯度。
5. 由于各品牌离心机的性能不同,实验室环境温度差异,可能影响分离效果,用户可以调节离心转数和离心时间,摸索最佳的分离条件(具体分离条件各实验室自定)。
6. 部分塑料制品(如聚苯乙烯)因其带有的静电作用,可能会导致细胞挂壁,影响分离效果。最好使用无静电反应的离心管,推荐使用未经过碱处理的玻璃离心管。
7. 细胞重悬样本的粘稠度或者是温度差异,可能会影响分离效果,可以调节离心转数和离心时间,请摸索最佳的分离条件。
8. 吸取过多的淋巴细胞层及分离液层会导致分离液交界处的粒细胞被吸出从而使混杂的粒细胞数量增加。
9. 不同动物的组织细胞重悬在不同比重分离液中的细胞离散系数及细胞带电不同,用户在制定分离液时应提供所需分离液的比重、动物种属及被分离细胞的名称。
可能存在的问题及解决方法:
1. 离心后目的细胞存在于血浆层或稀释液层,可能原因是转速过小或离心时间过短,解决办法可以适当增加转速。
2. 离心后目的细胞存在于分离液中,可能原因是转速过大或离心时间过长,解决办法可以适当减低转速。
3. 离心后白膜层弥散,可能原因是细胞密度过大,解决办法适当调整细胞密度。
4. 离心后白膜层太浅或看不见,可能原因是细胞密度过小,解决办法适当调整细胞密度。