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4′,6-联脒-2-苯基吲哚二盐酸盐

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上海金畔生物科技有限公司提供4′,6-联脒-2-苯基吲哚二盐酸盐 ，欢迎访问官网了解更多产品信息。

产品编号 D-9106
英文名称 DAPI
中文名称 4′,6-联脒-2-苯基吲哚二盐酸盐
别    名 4′,6-Diamidino-2-phenylindole, dihydrochloride; DAPI dihydrochloride; 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; CAS:47165-04-8;   核酸染料;
4
Specific References  (33)     |     D-9106 has been referenced in 33 publications.
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保存条件 Store at -20℃, protect from light.
产品介绍 DAPI, or 4′,6-diamidino-2-phenylindole, is a fluorescent stain that binds strongly to adenine–thymine rich regions in DNA. It is used extensively in fluorescence microscopy. As DAPI can pass through an intact cell membrane, it can be used to stain both live and fixed cells, though it passes through the membrane less efficiently in live cells and therefore the effectiveness of the stain is lower.

DAPI基本信息：
　　级别/纯度：BR，98%
　　CAS：28718-90-3
　　分子式： C16H15N5·2HCl
　　分子量： 350.25
　　MDL： MFCD00012681

DAPI性质：
　　1. 外观：黄色粉末
　　2. 纯度：≥95% (HPLC)
　　3. 产品描述：
　　DAPI是一种可对DNA 染色的细胞核染色试剂，它在嵌入双链DNA后释放蓝色荧光。DAPI常用于细胞凋亡检测，染色后用荧光显微镜观察或流式细胞仪检测。尽管DAPI不能通过活细胞膜，但却能穿透扰乱的细胞膜而对核染色。DAPI具有很高的光漂白承受水平，能用来检测酵母线粒体DNA，叶绿体DNA，病毒DNA，microplasm DNA以及染色体DNA。DAPI-DNA复合物的激发和发射波长分别为360nm和460nm。
　　4. 染色过程
　　(1) 用1mL ddH2O将DAPI溶解，制得2.9mM的DAPI溶液(1mg DAPI/1mL H2O)。
　　注：DAPI不能直接用PBS等缓冲溶液溶解，需要先用水将其溶解。
　　(2) 取适量DAPI水溶液加到PBS中，制备成10～50µM的DAPI溶液。
　　推荐以下PBS的配制方法：
　　将8.0g NaCl、0.2g KCl、2.9g Na2HPO4·12H2O、0.2g KH2PO4 溶解于1L纯水中。
　　(3) 将1/10培养基体积的DAPI溶液加入到细胞培养基中。也可以用1/10浓度的DAPI缓冲液代替培养基。
　　(4) 在37℃培养细胞10～20分钟。
　　(5) 用PBS或合适的缓冲液洗细胞两次。
　　(6) 用带有360nm激发波长，460nm发射波长的滤光片的荧光显微镜观察细胞。

　　储存条件：-20℃干燥避光保存，有效期一年。

　　注意事项：
　　DAPI对人体有一定刺激性，请注意适当防护。
　　荧光染料都存在淬灭的问题，建议染色后尽量当天完成检测。
　　为减缓荧光淬灭可以使用抗荧光淬灭封片液。