上海金畔生物科技有限公司提供溶葡萄球菌酶 (≥90%, ≥3000 unit/mg) ,欢迎访问官网了解更多产品信息。
产品编号 | D10379 |
英文名称 | Lysostaphin from Staphylococcus simulans |
中文名称 | 溶葡萄球菌酶 (≥90%, ≥3000 unit/mg) |
别 名 | Glycyl-glycine Endopeptidase; Lysostaphin from Staphylococcus simulans (≥90%, ≥3000 unit/mg); Lysostaphin from Staphylococcus simulans, Recombinant; Lysostaphin, Recombinant (Staphylococcus simulans); 溶葡球菌酶; 重组溶葡萄球菌酶; |
保存条件 | Store at -20℃. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品介绍 | CAS号:9011-93-2 分子量:27kDa MDL:MFCD00131556 级别:BR 活性:≥3000 unit/mg solid 纯度:≥90% (HPLC) 外观/性状:lyophilized powder 酶活性定义:One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37°C in a 6.0 ml reaction mixture. 等电点:10.5-11.0。 酶活性最适pH范围:10~11。 影响因素:羟汞苯甲酸(PCMB)、 苯甲磺酰氯(PMSF)、二硫苏糖醇(DTT) 等在1mmol/L的条件下,对酶活力无明显影响,Zn离子表现出某种程度的活化效应;Ba离子、Mn离子、Ca离子、Mg离子和EDTA在1mmol/L的条件下,对溶葡球菌酶表现出一定程度的抑制作用 溶解性:可溶于50mmol/L Tris 缓冲液 来源:Recombinant, Expressed in E. coli. 用途:生化研究。专一降解金黄色葡萄球菌等细胞壁具有甘氨酸肽键结构的细菌,通过裂解其细胞壁达到杀菌的目的。在pH值为4和5℃时高度稳定。 产品描述:Lysostaphin from Staphylococcus simulans has been used in a study to assess molecular cloning, sequencing, and expression of lytM, a unique autolytic gene of Staphylococcus aureus. It has also been used in a study to investigate the sequence analysis of a Staphylococcus aureus gene encoding a peptidoglycan hydrolase activity. A protein complex with highly specific lytic activity against Staphylococcus species, including Staphylococcus aureus. Lysostaphin possesses a lytic action against Staphylococcus aureus, which is used to treat antibiotic-resistant staphylococcal infections. It has the properties of enzymes, such as glycylglycine endopeptidase, endo-β-N-acetyl glucosamidase and N-acetyl muramyl-L-alanine amidase. Lysostaphin is also used as a preservative in food industry and in clinical labs for rapid screening. The antimicrobial activity of lysostaphin, a metalloendopeptidase expressed by Staphylococcus simulans, is enhanced by binding to the C′ terminus of the membrane-associated Trap protein of Staphylococcus aureus. |