# R300
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产品资料
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货号
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R300
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同种型(isotype)
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大鼠IgG (Wistar)
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含量
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0.5 mg purified immunoglobulins in PBS, sterile filtered
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包装
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1ML
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浓度
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0.5 mg/ml
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本产品只用于科研,不用于诊断,也不属于医疗用品
特异性: This antibody preparation contains a mixture of purified rat monoclonal antibodies directed against mouse GPIba (CD42b). Targeting of this receptor with divalent IgGs results in profound and irreversible Fc-independent plaet depletion in mice1,2. Plaet depletion is frequently used to study the general role of plaets in (patho-) physiology3.
制备 : 抗体是杂交瘤细胞上清通过G-Sepharose纯化所得,内毒素含量小于 0.0025 EU/µg
保存和运输:4度保存和运输,保质期为6个月 ,无菌保存不含防腐剂,分装保存-20度zui少可以保存一年以上,但请避免反复冻融
使用方法: 按动物体重注射抗体 量为2 µg抗体/g动物体重60分钟内可以去除血小板95%以上,建议每次注射100-200µL抗体(用无菌PBS稀释)。
引用文献: 1. Bergmeier W, Rackebrandt K, Schroder W, Zirngibl H, Nieswandt B. (2000) Structural and functional characterization of the mouse von Willebrand factor receptor GPIb-IX with novel monoclonal antibodies. Blood. 95:886-93.
2. Nieswandt B, Bergmeier W, Rackebrandt K, Gessner JE, and Zirngibl H. (2000) Identification of critical antigen-specific mechanisms in the development of immune thrombocytopenic purpura in mice. Blood. 96:2520-27.
3. Elzey BD, Tian J, Jensen RJ et al. (2003) Plaet-mediated modulation of adaptive immunity. A communication link between innate and adaptive immune compartments. Immunity.19:9-19.
: :fige007