德国dsmz ACC 757说明书

德国dsmz ACC 757说明书

 

JA

ACC 757

细胞系:

JA

DSMZ号:

ACC 757

种类:

人(智人

单元格类型:

伯基特淋巴瘤

起源:

于1973年从一名EBV阴性的5岁非洲女孩腹股沟淋巴瘤的腹股沟肿瘤的末端建立;尽管肿瘤组织通过实验暴露于EBV,但在已建立的细胞系中均未检测到EBV序列或EBV抗原。提供外显子组和RNA序列数据(请参见Ref 18187和外 显子组序列 和RNA-Seq)

参考文献:

18899,15230,18187

生物安全等级:

1个

权限和限制:

 

形态学:

<a data-cke-saved-href=”https://www.dsmz.de/collection/catalogue/human-and-animal-cell-lines/permissions-/-restrictions” href=”https://www.dsmz.de/collection/catalogue/human-and-animal-cell-lines/permissions-/-restrictions” “=”” class=”internal-link-new-window” style=”box-sizing: border-box; color: rgb(48, 76, 178); text-decoration-line: none;”>四舍五入为在单个细胞和团块中生长的多态细胞;形象; 图片

中:

80%RPMI 1640 + 20%高FBS

亚文化:

播种在约。0.5-0.7 x 10 6细胞/ ml; 每2-3天宜分割比例1:2 -1:5;培养瓶应保持水平

孵化:

在37°C和5%CO 2下

时间加倍:

ca. 30-40小时

收成:

大密度约 1.5 x 10 6细胞/ ml

存储:

用70%培养基,20%FBS,10%DMSO冷冻

 

DSMZ科学数据:

支原体:

PCR检测阴性

免疫学:

CD3-,CD10 +,CD13-,CD19 +,CD20 +,CD34-,CD37 +,CD38 +,HLA-DR +,sm / cyIgG-,sm / cyIgM +,sm / cykappa +,sm / cylambda-; 

指纹:

基因分型显示了短串联重复序列(STR)的模式,与STR数据库的参考资料相符

种类:

人的

细胞遗传学:

人二倍体核型;44(40-46)2n XX,-10,-21,der(6)t(6; 10)(p22; p12),der(7)t(1; 7)(p36; p22),t (11; 17)(q24; q24),der(11)t(11; 17)(q24; q24)t(11; 6)(p15; q11)t(6; 5)(q22 ;?) (13)(q10)t(3; 13)(q26; q14),der(13)t(10; 13)(q11; p11)dup(13)(q11q32)t(13; 21)(q32; q22 ),der(21)t(13; 21); 与先前发表的BJA-B核型不符;未检测到IG / MYC易位

病毒:

PCR:EBV-,HBV-,HCV-,HIV-1-,HIV-2-,HTLV-I / -II-,MLV-

提供为:

交付形式   价格
 
冷冻文化   400欧元
文化的发展
  (请询问确切的交货时间)
  800欧元
从细胞系中分离的DNA(25 µg)   500欧元
从细胞系中分离的DNA(5微克)  

120欧元

Agdia ACC 00150说明书

上海金畔生物为Agdia国内一级代理商,Agdia公司成立于1981年,agdia 公司是植物病害检测试剂生产商,产品种类达200余种,包括试剂盒及试纸条,操作简便,灵敏,包装规格齐全,不同的包装规格适合不同规模的实验室,受到国内外客户的认可。

 

Agdia ACC 00150说明书

货号:ACC 00150

品名:AmplifyRP® Acceler8®

有可能的使用:

所述AmplifyRP ®起动机组包括所有必要执行AmplifyRP设备® Acceler8 ®反应。

包括:

便携式加热块:该AmplifyRP ®便携式热块能够以恒定39℃保持达40个反应。该装置体积小巧,重量仅为1.2磅,可用于实验室或现场环境。如果在现场使用,则需要电源转换器。

 

5μL,10μL25μL&微型移液器:小可重复使用的固定体积的移液管用于地输送所需AmplifyRP ® Acceler8 ®试剂。

 

Agdia其它产品目录:

Alfalfa Mosaic Virus(AMV)苜蓿花叶病毒诊断试剂

African Cassava Mosaic Virus (ACMV)非洲木薯花叶病毒诊断试剂

Acidovorax avenae subsp.citrulli (Aac) 燕麦噬酸菌诊断试剂

American Plum Line Pattern Virus (APLPV)美洲李子线状病毒诊断试剂

Andean Potato Latent Virus(APLV)安第斯马铃薯潜隐性病毒诊断试剂

Angelonia flower break virus (AnFBV) 天使花花碎病毒诊断试剂

Apple Chlorotic Leaf spot Virus (ACLSV)Sanofi ELISA 苹果褪绿叶斑病毒诊断试剂

Arabis Mosaic Virus(ArMV)南芥菜花叶病毒诊断试剂

Asparagus Virus2  (AV2)芦笋病毒2诊断试剂

Banana Bract Mosaic Virus (BBrMV)香蕉苞花叶病毒诊断试剂

Banana Bunchy Top Virus (BBTV)香蕉束顶病毒诊断试剂

Barley stripe Mosaic Virus (BSMV)大麦条纹花叶病毒诊断

 

 

 

 

货 号

品 名

规 格

单价(元)

厂 家

SRA18200/0500

Maize White Line Mosaic Virus(玉米白线花叶病毒)

500孔

3360

Agdia

SRA62300/0500

Lily Symptomless Virus(百合无症状病毒)

500孔

3360

Agdia

SRA68000/0500

Carnation Mottle Virus(康乃馨斑点病毒)

500孔

3360

Agdia

SRA88500/0500

Papaya Mosaic Virus(番木瓜花叶病毒)

500孔

3360

Agdia

PSA20101/0096

Onion Yellow Dwarf Virus(洋葱黄色矮小病毒)

96孔

2350

Agdia

SRA54300/0500

Odontoglossum Ringspot Virus(齿舌兰环斑病毒)

500孔

3360

Agdia

SRA24300/0500

Nemesia ring necrosis virus(龙面花环疽病毒)

500孔

4760

Agdia

SRA68300/0500

Carnation Ringspot Virus(康乃馨环斑病毒)

500孔

3360

Agdia

SRA12401/0500

Melon Necrotic Spot Virus(瓜坏死斑点病毒)

500孔

3360

Agdia

SRP45600/0500

Cauliflower Mosaic Virus(花椰菜花叶病毒)

500孔

3360

Agdia

SRA17300/0500

Maize Stripe Virus(玉米斑纹病毒)

500孔

3360

Agdia

SRA18800/0500

Maize Streak Virus(玉米条纹病毒)

500孔

3360

Agdia

SRA18900/0500

Maize Mosaic Virus(玉米花叶病毒)

500孔

3360

Agdia

SRA18000/0500

Maize Dwarf Mosaic Virus(玉米矮小花叶病毒)

500孔

3360

Agdia

SRA17001/0500

Maize Chlorotic Mottle Virus(玉米枯黄斑点病毒)

500孔

3360

Agdia

PSA20500/0096

Impatiens Necrotic Spot Virus(凤仙坏死斑点病毒)

96孔

2350

Agdia

SRA72900/0500

Nandina Mosaic Virus(南天竹花叶病毒)

500孔

3360

Agdia

SRA89500/0500

Cymbidium Mosaic Virus(兰花花叶病毒)

500孔

3360

Agdia

SRA73600/0500

African Cassava Mosaic Virus(非洲木薯花叶病毒)

500孔

3360

Agdia

SRA12300/0500

Hibiscus Chlorotic Ringspot Virus(芙蓉枯黄环斑病毒)

500孔

3360

Agdia

PSA61000/0096

Groundnut Ringspot Virus – and Tomato Chlorotic Spot Virus(花生卷叶病毒和番茄枯黄斑点病毒)

96孔

2250

Agdia

PSA62800/0096

Groundnut Bud Necrosis Virus and Watermelon Silver Mottle Virus(花生胚芽坏死病毒及西瓜银斑病毒)

96孔

2350

Agdia

SRA27701/0500

Grapevine Fanleaf Virus(葡萄扇叶病毒)

500孔

3360

Agdia

SRA20400/0500

Garlic Common Latent Virus(蒜头普通潜伏病毒)

500孔

3360

Agdia

SRA68500/0500

Carnation Necrotic Fleck Virus(康乃馨坏死斑点病毒)

500孔

3360

Agdia

SRA33001/0500

Cymbidium Ringspot Virus(兰花环斑病毒)

500孔

3360

Agdia

SRA45800/0500

Lettuce Mosaic Virus(莴苣花叶病毒)

500孔

3360

Agdia

PSA44800/0096

Cucumber Mosaic Virus-Subgroup II (黄瓜花叶病毒-亚群II)

96孔

2350

Agdia

PSA44700/0096

Cucumber Mosaic Virus-Subgroup I (黄瓜花叶病毒-亚群I)

96孔

2350

Agdia

PSA44501/0096

Cucumber Mosaic Virus (黄瓜花叶病毒)

96孔

2350

Agdia

SRA45700/0500

Cucumber Green Mottle Mosaic Virus(黄瓜绿色斑点花叶病毒)

500孔

3360

Agdia

SRA24200/0500

Cowpea Mosaic Virus(豇豆花叶病毒)

500孔

3360

Agdia

PSA81000/0096

Chrysanthemum Virus B(菊花病毒B)

96孔

2350

Agdia

SRA48000/0500

Dasheen Mosaic Virus(芋头花叶病毒)

500孔

3360

Agdia

PSA27500/0096

Barley Yellow Dwarf Virus-pav(大麦黄矮病毒-pav)

96孔

2350

Agdia

SRA17401/0500

Maize Chlorotic Dwarf Virus-strain T(玉米枯黄矮小病毒T株)

500孔

3360

Agdia

SRA88001/0500

Blueberry Leaf Mottle Virus(蓝莓叶斑点病毒)

500孔

3780

Agdia

SRA32100/0500

Beet Western Yellows Virus(甜菜Western黄色病毒)

500孔

3360

Agdia

SRA16301/0500

Beet Necrotic Yellow Vein Virus(甜菜坏死黄色叶脉病毒)

500孔

3360

Agdia

PSA46400/0096

Bean Pod Mottle Virus(豆荚斑点病毒)

96孔

2350

Agdia

PSA46000/0096

Bean Common Mosaic Virus(豆普通花叶病毒)

96孔

2350

Agdia

SRA19200/0500

Blueberry Shock Virus(蓝莓Shock病毒)

500孔

3360

Agdia

SRA28001/0500

Barley Yellow Dwarf Virus-rpv(大麦黄矮病毒-rpv)

500孔

3360

Agdia

SRP19000/0500

Blueberry Shoestring Virus(蓝莓鞋带病毒)

500孔

3360

Agdia

PSA23201/0096

Arabis Mosaic Virus(筷子芥花叶病毒)

96孔

2350

Agdia

PSA30700/0096

Apple Mosaic Virus(苹果花叶病毒)

96孔

2350

Agdia

SRA40200/0500

Andean Potato Latent Virus(安第斯马铃薯潜伏病毒)

500孔

2170

Agdia

SRA32000/0500

American Plum Line Pattern Virus(美国李子线状病毒)

500孔

3360

Agdia

SRA39000/0500

Alstroemeria Mosaic Virus(Alstoemeria花叶病毒)

500孔

3360

Agdia

PSA87600/0096

Alfalfa Mosaic Virus(苜蓿花叶病毒)

96孔

2350

Agdia

SRA27000/0500

Barley Yellow Dwarf Virus-sgv(大麦黄矮病毒-sgv)

500孔

3360

Agdia

PSB05600/0096

Bt-Cry9C protein

96孔

2660

Agdia

SRA20301/0500

Leek Yellow Stripe Virus(韭菜黄色斑纹病毒)

500孔

3360

Agdia

SRA65000/0500

Kyuri Green Mottle Mosaic Virus(Kyuri绿色斑点花叶病毒)

500孔

3360

Agdia

SRA62000/0500

Kalanchoe Latent Virus(高凉菜潜伏病毒)

500孔

3360

Agdia

SRA18300/0500

Johnsongrass Mosaic Virus(Johnsongrass花叶病毒)

500孔

3360

Agdia

SRA60500/0500

Iris Yellow Spot Virus(Iris黄斑病毒)

500孔

3360

Agdia

SRA34000/0500

Carnation Latent Virus(康乃馨潜伏病毒)

500孔

3360

Agdia

SRA19100/0500

Blueberry Scorch Virus(蓝莓枯黄病毒)

500孔

3360

Agdia

PSA83000/0096

Calibrachoa Mottle Virus

96孔

2350

Agdia

PSA53500/0096

Papaya Ringspot Virus(番木瓜环斑病毒)

96孔

2350

Agdia

PSP06100/0096

Bt-Cry3Bb1 protein

96孔

2660

Agdia

PSA05900/0096

Bt-Cry3A protein

96孔

2660

Agdia

PSB05800/0096

Bt-Cry2A protein

96孔

2660

Agdia

PSB05700/0096

Bt-Cry1C protein

96孔

2660

Agdia

PSP06200/0096

Bt-Cry1Ab/Ac protein

96孔

2660

Agdia

SRA29300/0500

Brome Mosaic Virus(雀麦花叶病毒)

500孔

3360

Agdia

SRA34100/0500

Carnation Etched Ring Virus(康乃馨风化环病毒)

500孔

3360

Agdia

PSA71000/0096

Asparagus Virus 2(芦笋病毒2)

96孔

2350

Agdia

PSA22000/0096

Tomato Ringspot Virus(番茄环斑病毒)

96孔

2350

Agdia

PSA20700/0096

Potato Virus Y-strains c(马铃薯病毒Y-c株)

96孔

2350

Agdia

PSA20001/0096

Potato Virus Y(马铃薯病毒Y)

96孔

2350

Agdia

PSA10000/0096

Potato Virus X(马铃薯病毒X)

96孔

2350

Agdia

SRA10100/0096

Potato Virus V(马铃薯病毒V)

500孔

2170

Agdia

SRA40100/0500

Potato Virus T(马铃薯病毒T)

500孔

2170

Agdia

PSA26001/0096

Potato Virus Y-necrotic strain(马铃薯病毒Y-坏死株)

96孔

2350

Agdia

SRA24600/0500

Banana Bract Mosaic Virus(香蕉苞叶花叶病毒)

500孔

3360

Agdia

PSA27200/0096

Potyvirus Group(马铃薯Y病毒属)

96孔

2350

Agdia

PSA47001/0096

Wheat Streak Mosaic Virus(小麦条纹花叶病毒)

96孔

2350

Agdia

SRA43001/0500

Wheat Spindle Streak Mosaic Virus(小麦纺锤状条纹花叶病毒)

500孔

3360

Agdia

SRA61500/0500

Watermelon Silver Mottle Virus – and Groundnut Bud Necrosis Virus(西瓜银斑病毒和花生芽坏死病毒)

500孔

3360

Agdia

SRA54000/0500

Watermelon Mosaic Virus 2(西瓜花叶病毒2)

500孔

3360

Agdia

SRA18700/0500

Turnip Mosaic Virus(芜箐花叶病毒)

500孔

3360

Agdia

SRA16600/0500

Hosta Virus X(玉簪属植物病毒X)

500孔

3360

Agdia

PSA40000/0096

Potato Virus S(马铃薯病毒S)

96孔

2350

Agdia

SRA34600/0500

Shallot Yellow Stripe Virus(葱黄色斑纹病毒)

500孔

3360

Agdia

SRA19500/0500

Barley Stripe Mosaic Virus(大麦条状花叶病毒)

500孔

3360

Agdia

SRA24700/0500

Banana Bunchy Top Virus(香蕉串状顶病毒)

500孔

3360

Agdia

PSA77700/0096

Zucchini Yellow Mosaic Virus(南瓜黄色花叶病毒)

96孔

2350

Agdia

SRA14000/0500

Strawberry Latent Ringspot Virus(草莓潜伏环斑病毒)

500孔

3360

Agdia

PSA26400/0096

Squash Mosaic Virus(南瓜花叶病毒)

96孔

2350

Agdia

PSA33300/0096

Soybean Mosaic Virus(大豆花叶病毒)

96孔

2350

Agdia

PSA20600/0096

Potato Virus Y-strains o and c(马铃薯病毒Y-o和c株)

96孔

2350

Agdia

SRA42001/0500

Soil-Borne Wheat Mosaic Virus(土壤传播小麦花叶病毒)

500孔

3360

Agdia

PSA35400/0096

Tomato Mosaic Virus(番茄花叶病毒)

96孔

2350

Agdia

SRP34200/0500

Shallot Latent Virus(葱潜伏病毒)

500孔

3360

Agdia

PSA57600/0096

Ribgrass Mosaic Virus(长叶车前草花叶病毒)

96孔

2350

Agdia

SRA59401/0500

Raspberry Ringspot Virus(Raspberry环斑病毒)

500孔

3360

Agdia

SRA58200/0500

Raspberry Bushy Dwarf Virus(悬钩子茂密矮小病毒)

500孔

3360

Agdia

PSA30500/0096

Prunus Necrotic Ringspot Virus(野黑樱桃坏死环斑病毒 )

96孔

2350

Agdia

PSA98700/0096

Prune Dwarf Virus(洋李矮小病毒)

96孔

2350

Agdia

SRA24100/0500

Southern Bean Mosaic Virus(南方菜豆花叶病毒)

500孔

3360

Agdia

PSA37500/0096

Pepper Mild Mottle Virus(胡椒温和斑点病毒)

96孔

2350

Agdia

PSA39300/0096

Tomato Spotted Wilt Virus(番茄斑点枯萎病毒)

96孔

2350

Agdia

SRA33602/0500

Plum Pox Virus(李子痘病毒)

500孔

3780

Agdia

PDK09351/0096

Isopentenyladenosine(异戊烯腺苷)

96孔

5925

Agdia

PDK09349/0096

Dihydrozeatin(双氢玉米素)

96孔

5925

Agdia

PDK09348/0096

trans-Zeatin Riboside(反-玉米素核糖苷)

96孔

5925

Agdia

PDK09347/0096

Abscisic Acid(脱落酸)

96孔

5925

Agdia

SRA21000/0500

Potato Aucuba Mosaic Virus(马铃薯珊瑚状花叶病毒)

500孔

2170

Agdia

SRA38000/0500

Pepper Mottle Virus(胡椒斑点病毒)

500孔

3360

Agdia

SRA40501/0500

Potato Latent Virus(马铃薯潜伏病毒)

500孔

2170

Agdia

PSA13000/0096

Pepino Mosaic Virus(Pepino花叶病毒)

96孔

2350

Agdia

PSA90100/0096

Pelargonium Zonate Spot Virus(天竺葵带状斑点病毒)

96孔

2350

Agdia

SRA92700/0500

Pelargonium Leaf Curl Virus(天竺葵叶卷曲病毒)

500孔

3360

Agdia

SRA90000/0500

Pelargonium Flower Break Virus(天竺葵花破裂病毒)

500孔

3360

Agdia

SRA44600/0500

Peanut Stunt Virus(花生矮小病毒)

500孔

3360

Agdia

SRA87700/0500

Peach Rosette Mosaic Virus(桃子玫瑰形花饰花叶病毒)

500孔

3360

Agdia

PDK09346/0096

Indole-3-acetic Acid(吲哚-3-乙酸)

96孔

5925

Agdia

PSA49500/0096

Tobacco Etch Virus(烟草蚀刻病毒)

96孔

2350

Agdia

SRA45400/0500

Tomato Bushy Stunt Virus(番茄茂密矮小病毒)

500孔

3360

Agdia

PSA37000/0096

Tomato Aspermy Virus(番茄Aspermy病毒)

96孔

2350

Agdia

SRA46300/0500

Tobacco Vein Mottling Virus(烟草叶脉斑点病毒)

500孔

3360

Agdia

PSA25500/0096

Tobacco Streak Virus(烟草条纹病毒)

96孔

2350

Agdia

PSA64000/0096

Tobacco Ringspot Virus(烟草环斑病毒)

96孔

2350

Agdia

SRA25000/0500

Tobacco Rattle Virus(烟草Rattle病毒)

500孔

3360

Agdia

SRA90700/0500

Poinsettia Mosaic Virus(猩猩木花叶病毒)

500孔

3360

Agdia

PSA57400/0096

Tobacco Mosaic Virus(烟草花叶病毒)

96孔

2350

Agdia

PSA26500/0096

Barley Yellow Dwarf Virus-mav(大麦黄矮病毒-mav)

96孔

2350

Agdia

SRA18100/0500

Sugarcane Mosaic Virus(甘蔗花叶病毒)

500孔

3360

Agdia

SRA72200/0500

Sugarcane Bacilliform Virus(甘蔗杆状病毒)

500孔

3360

Agdia

SRA14500/0500

Strawberry Mild Yellow Edge – associated Virus(草莓温和黄边相关病毒)

500孔

3360

Agdia

PSA50000/0096

Potato Virus M(马铃薯病毒M)

96孔

2350

Agdia

PSA60000/0096

Potato Virus A(马铃薯病毒A)

96孔

2350

Agdia

PSA30002/0096

Potato Leaf Roll Virus(马铃薯卷叶病毒)

96孔

2350

Agdia

PSA57500/0096

Tobacco Mosaic Virus-c(烟草花叶病毒-c)

96孔

2350

Agdia

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pQCXIH载体说明书


 pQCXIH载体说明书

型号 载体名称 出品公司 载体用途
VNC0418 pQCXIH Clontech 逆转录病毒载体

 

 

产品参数: 

Promotor: CMV/MSV
Size: 7800
5′ Sequencing 1 Primer: QC
5′ Sequencing 1 Primer Sequence: 5’d[ACGCCATCCACGCTGTTTTGACCT]3′
Tag 1: IRES-Hygro
Selectable Marker: Hygromycin
Viral/Non-Viral: Retroviral

载体抗性: 
氨苄青霉素(Ampicillin)
质粒图谱: 

 

Matchmaker™ Library Construction & Screening Kits (pGBKT7,pGADT7,pGBKT7-53,pGADT7-T,pGBKT7-Lam)

 

pGBKT7-53

 
产品参数: 

Bacteria Resistance:KanamycinMammalian Selection:-

载体抗性: 
卡那霉素(Kanamycin)
载体描述: 

载体特点:与pGADT7-T构成Matchmaker Two-Hybrid System 3的双杂交正对照。

pGBKT7-53 encodes a fusion of the murine p53 protein (a.a. 72–390) and the GAL4 DNA-BD (a.a. 1–147). The murine p53 cDNA (GenBank Accession #K01700) was cloned into pGBKT7 at the EcoR I and BamH I sites. The p53 insert was derived from the plasmid described in Iwabuchi et al. (1993); plasmid modification was performed at Clontech. pGBKT7-53 has not been sequenced.

质粒图谱: 

 

pGBKT7-Lam

产品参数: 

Bacteria Resistance:KanamycinMammalian Selection:-

载体抗性: 
卡那霉素(Kanamycin)
载体描述: 

载体特点:Matchmaker Two-Hybrid System 3的负对照。

pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein (a.a. 66–230) and the GAL4 DNA-BD (a.a. 1–147). The lamin C cDNA insert (GenBank Accession #M13451) was derived from the plasmid referenced in Bar et al. (1993a). Plasmid modification was performed atBD Biosciences Clontech. Yeast cotransformed with pGBKT7-Lam and pGADT7-RecT, provide a measure of the backgroundthat is due to false-positive two-hybrid interactions. pGBKT7-Lam has not been sequenced.

质粒图谱: