上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
Universal miRNA Cloning Linker
- This 5´ adenylated, 3´ blocked oligodeoxynucleotide can be used for cloning short RNAs according to the procedure of Bartel (1).
- RNA ligase recognizes the “activated” adenylated oligo and covalently ligates its 5´ end to the 3´ OH of a second single stranded sequence in the absence of ATP.
- In a mixture of nucleic acids use of the 5´ adenylated, 3´ blocked oligo with RNA ligase (w/o ATP) results in ligation of the target oligo only.
- Sequence: 5′ rAppCTGTAGGCACCATCAAT–NH2 3′
Thermostable 5´ App DNA/RNA Ligase |NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
Thermostable 5´ App DNA/RNA Ligase
Thermostable 5´ App DNA/RNA Ligase ligates a 5´ pre-adenylated 3´-blocked DNA linker to the 3´-OH of RNA and ssDNA.
- Isolated from a recombinant source
- Supplied with 10X reaction buffer
- Tested for the absence of DNases and RNases
- Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart
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DNA Ligation
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5′ Deadenylase |NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
5´ Deadenylase
5´ Deadenylase is capable of deadenylation from 5´-end of DNA and RNA, leaving the phosphate at 5´-end. It also cleaves AppppA into ATP and AMP.
- Isolated from a recombinant source
- Supplied with 10X reaction buffer
- Tested for the absence of DNases, RNases, and phosphatases
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Avoiding RNase Contamination
XRN-1 |NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
XRN-1
New size
XRN-1 is highly processive 5´→3´ exoribonuclease, requiring 5´ monophosphate. It also acts on 5´ monophosphate ssDNA with greatly reduced efficiency.
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
- Tested for the absence of DNases and RNases