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pRevTet-off说明书

发表于由

 

pRevTet-off

型号 载体名称 出品公司 载体用途
VSC0470 pRevTet-off Clontech 四环素调控系统
载体描述: 

pRevTet-Off™ is a retroviral vector expressing the tetracycline-controlled transactivator （tTA） from the CMV promoter. This vector is derived from pLNCX, a retroviral vector created using elements of Moloney murine leukemia virus （MoMuLV） and Moloney murine sarcoma virus （MoMuSV） as described. tTA is a fusion of amino acids 1–207 of the tet repressor （TetR） and the negatively charged C-terminal activation domain （130 amino acids） of the VP16 protein of Herpes Simplex Virus. The 5′ viral LTR controls expression of the transcript that contains Y+ （the extended viral packaging signal）, and the neomycin resistance gene （Neor） for antibiotic selection in mammalian cells. tTA is derived from vectors described previously. pRevTet-Off also includes the E. coli Ampr gene for antibiotic selection in bacteria. pRevTet-Off can be used to establish stable Tet-Off cell lines via retrovirus-mediated gene transfer. Retroviral gene transfer allows the highly efficient transduction of virtually all dividing cell types. The RevTet™ Systems are also suitable for establishing transgenic animals. In combination with the pRev-TRE retroviral expression vector, a gene of interest can be inducibly expressed at high levels in response to varying concentrations of tetracycline （Tc） or Tc derivatives such as doxycycline （Dox）. tTA binds to the Tet-response element （TRE）, thus activating transcription in the absence of Tc or Dox. As Tc or Dox is added to the culture medium, transcription from the inducible promoter is turned off in a highly dose-dependent manner. pRevTet-Off lacks the viral genes gag, pol, and env, which are supplied by the packaging cell line. It can be transfected into a high titer packaging cell line and thereby mediate production of infectious, replication-incompetent retroviral particles .The transcript produced by the pRevTet-Off construct is recognized by the viral structural proteins expressed in a packaging cell line and packaged into infectious retroviral particles. Because the RNA transcript packaged in these particles does not contain the viral genes, it cannot replicate in the target cells that it infects. The level of induction in cell populations infected with this vector depends on the efficiency of infection, the site of integration, and the titer of the virus. Viral supernatants with titers >105 cfu/ml should be produced to achieve high-level induction.

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pRevTet-On 说明书

发表于由

四环素调控系统 pRevTet-On

pRevTet-On

型号 载体名称 出品公司 载体用途
VSC0469 pRevTet-On Clontech 四环素调控系统

 

Description:

pRevTet-On is a retroviral vector expressing the reverse tetracycline-controlled transactivator 

（rtTA） from the CMV promoter. This vector is derived from pLNCX, a retroviral vector created 

using elements of Moloney murine leukemia virus （MoMuLV） and Moloney murine sarcoma 

virus （MoMuSV） as described （1）. rtTA is a fusion of amino acids 1–207 of the reverse tet 

repressor （rTetR） and the negatively charged C-terminal activation domain （130 amino acids） 

of the VP16 protein of Herpes Simplex Virus. rTetR was derived from TetR and differs by four 

point mutations, which are responsible for its opposite response to doxycycline. The 5′ viral 

LTR controls expression of the transcript that contains Ψ+

 （the extended viral packaging signal） 

and the neomycin resistance gene （Neor

） for antibiotic selection in mammalian cells. rtTA is 

derived from vectors described previously （2–4）. pRevTet-On also includes the E. coli Ampr

gene for antibiotic selection in bacteria.

Use:

pRevTet-On can be used to establish stable Tet-On cell lines via retrovirus-mediated gene 

transfer （5）. Retroviral gene transfer allows the highly efficient transduction of virtually all 

dividing cell types. The RevTet Systems are also suitable for establishing transgenic animals. In 

combination with the pRev-TRE retroviral expression vector, a gene of interest can be inducibly 

expressed at high levels in response to varying concentrations of the tetracycline derivative 

doxycycline （Dox）. rtTA binds to the Tet-response element （TRE）, thus activating transcription in the presence of Dox. The response of rtTA to Dox is thus opposite to the response of 

tTA. As Dox is removed from the culture medium, transcription from the inducible promoter 

is turned off in a highly dose-dependent manner. pRevTet-On lacks the viral genes gag, pol, 

and env, which are supplied by the packaging cell line. It can be transfected into a high titer 

packaging cell line and thereby mediate production of infectious, replication-incompetent 

retroviral particles （1, 6–7）.The transcript produced by the pRevTet-On construct is recognized 

by the viral structural proteins expressed in a packaging cell line and packaged into infectious 

retroviral particles. Because the RNA transcript packaged in these particles does not contain 

the viral genes, it cannot replicate in the target cells that it infects. 

The level of induction in cell populations infected with this vector depends on the efficiency 

of infection, the site of integration, and the titer of the virus. Viral supernatants with titers >105

cfu/ml should be produced to achieve high-level induction.

 

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Invitrogen pcDNA™6 TR说明书

发表于由

 pcDNA™6⁄TR

载体描述: 

The pcDNA™6⁄TR vector is part of the T-REx™ System, which yields higher levels of induced expression than any other regulated mammalian expression system. It utilizes the complete CMV promoter and adds control elements from the bacterial tetracycline resistance operon to effectively repress and derepress transcription from one of the strongest mammalian promoter sequences known.

The regulatory vector, pcDNA™6⁄TR, is provided for high-level expression of the tetracycline repressor （TR） protein. This vector expresses the Blasticidin resistance gene for rapid selection of mammalian cell lines that stably express the TR protein.

 

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3H Biomedical SCGK991M说明书

发表于由

Human Peripheral Blood Plasmacytoid Dendritic Cells/

3H Biomedical AB

瑞典三生细胞生物科技有限公司（3H Biomedical AB）是一家在同行业中处于位置的创新型生物科技研发企业。公司总部位于瑞典，乌普萨拉科技园区 (Uppsala Science Park)，自成立以来一直致力于研发和销售以人和动物细胞为基础的生物性测试系统。我公司的人或动物原代细胞产品可应用于药物毒性和个性化药物检测；组织工程研究；生命科学研究以及化学制剂和化妆品的安全性、过敏性检测。本公司产品专业供应于从事以上生命科学和生物学研究相关的各大学与研究所，生物制药企业，性制药企业和科研开发服务企业。产品销售遍及世界各地。

瑞典三生细胞生物科技有限公司可提供超过130种从正常或疾病组织中分离的原代细胞和其相关的RNA以及cDNA等产品。细胞类型包括干细胞，单核细胞，T细胞，B细胞，自然杀伤细胞，树突细胞，肝细胞等。

细胞系（cell line, 主要来源于ATCC）是发生突变，转化或具有永生性的细胞。与细胞系不同，原代细胞保留了原有细胞的特性，因此能够更好的模拟反映出在活体内细胞的形态，新陈代谢以及生长特性等。相比于动物实验，原代细胞实验更为仁道，快捷，灵敏，准确，不易受外界条件干扰。基于原代细胞的以上特性，使其成为模拟体内环境的体外研究中为理想的研究对象。

瑞典三生生物科技有限公司为顾客提供的血液细胞及其相关产品均为高质量，来源可靠的生命科学产品。采集过程均由受过专业训练经验丰富的技术人员执行操作，每一环节都符合瑞典国家血液采集法律规定。科学家亦可参照供血者的年龄，性别有针对性的选择产品。供血者均为志愿义务献血。各正常成年捐血者均经过健康测试，包括艾滋病检测（HIV test）,乙型肝炎检测（Hepatitis B test）和丙型肝炎检测（Hepatitis C test）。

 

Mouse cDNA Evaluation Kit, Deluxe, 100 reactions

小鼠cDNA评估试剂盒，Deluxe，100个反应

cDNA质量评估

ScienCell的GeneQuery™小鼠cDNA评估试剂盒，Deluxe（GQM-CED）评估小鼠cDNA质量。该试剂盒验证信使RNA（mRNA）成功逆转录成互补DNA（cDNA），揭示cDNA样品中基因组DNA（gDNA）污染的存在，并检测qPCR抑制剂污染。的cDNA是成功进行基因表达谱分析的关键组成部分。GQM-CED试剂盒强烈推荐用于cDNA应用，例如GeneQuery™qPCR阵列。（SCGK991M）

 

Human Primary Cells/人原代细胞

1. Peripheral Blood Cell System 外周血细胞系统
1.1 Human Peripheral Blood Mononuclear Cells/人外周血单核细胞
1.2 Human Peripheral Blood Pan-T Cells/人外周血Pan-T 细胞
1.3 Human Peripheral Blood CD4 T Cells/人外周血CD4 T 细胞
1.4 Human Peripheral Blood CD8 T Cells/人外周血CD8 T 细胞
1.5 Human Peripheral Blood B Lymphocytes/人外周血B 淋巴细胞
1.6 Human Peripheral Blood Natural Killer Cells/人外周血自然杀伤细胞
1.7 Human Peripheral Blood CD14 Monocytes/人外周血CD14 单核细胞
1.8 Human Monocyte-derived Macrophages/人单核细胞-衍生巨噬细胞
1.9 Human Peripheral Blood CD34 Stem Cells/人外周血CD34 干细胞
1.10 Human Peripheral Blood Monocyte-derived Immature Dendritic Cells/人外周血单核细胞-衍生未成熟树突细胞
1.11 Human Peripheral Blood Plasmacytoid Dendritic Cells/人外周血浆样树突细胞
1.12 Human Peripheral Blood Myeloid Dendritic Cells/人外周血髓样树突细胞
1.13 Human Peripheral Blood Endothelial Progenitor Cells /人外周血内皮祖细胞
1.14 Human Peripheral Blood Eosinophils/人外周血嗜酸性粒细胞
1.15 Human Peripheral Blood Basophils/人外周血嗜碱性粒细胞
1.16 Human Peripheral Blood Neutrophils/人外周血嗜中性白细胞
1.17 Human Peripheral Blood Platelets/人外周血血小板

2. Umbilical Cord Blood Cell System /脐带血细胞系统
2.1 Human Umbilical Cord Blood Mononuclear Cells/人脐带血单核细胞
2.2 Human Umbilical Cord Blood Pan-T Cells/人脐带血Pan-T 细胞
2.3 Human Umbilical Cord Blood CD4 T Cells/人脐带血CD4 T 细胞
2.4 Human Umbilical Cord Blood CD8 T Cells/人脐带血CD8 T 细胞
2.5 Human Umbilical Cord Blood B Lymphocytes/人脐带血B 淋巴细胞
2.6 Human Umbilical Cord Blood Natural Killer Cells/人脐带血自然杀伤细胞
2.7 Human Umbilical Cord Blood CD14 Monocytes/人脐带血CD14 单核细胞
2.8 Human Umbilical Cord Blood CD34 Stem Cells/人脐带血CD34 干细胞
2.9 Human Umbilical Cord Blood CD133 Stem Cells/人脐带血CD133 干细胞
2.10 Human Umbilical Cord Blood Mesenchymal Stem Cells/人脐带血间充质干细胞
2.11 Human Umbilical Cord Blood Monocyte-derived Immature Dendritic Cells/人脐带血单核细胞-衍生未成熟树突细胞
2.12 Human Umbilical Cord Blood Plasmacytoid Dendritic Cells/人脐带血浆样树突细胞
2.13 Human Umbilical Cord Blood Myeloid Dendritic Cells/人脐带血髓样树突细胞
2.14 Human Umbilical Cord Blood Endothelial Progenitor Cells/人脐带血内皮祖细胞
2.15 Human Umbilical Cord Blood Eosinophils/人脐带血嗜酸性粒细胞
2.16 Human Umbilical Cord Blood Basophils/人脐带血嗜碱性粒细胞
2.17 Human Umbilical Cord Blood Neutrophils/人脐带血嗜中性白细胞

人脉络丛内皮细胞

3. Hepatic Cell System/肝细胞系统
3.1 Fresh Human Hepatocytes /新鲜人肝细胞
3.2 Frozen Human Hepatocytes/冻存人肝细胞
3.3 Related Human Hepatic Cell Products/人肝细胞相关产品

Human Cell Lines/人类细胞株

4. Human Hepatic Cell Lines/人肝细胞株
4.1 HepaRG
4.2 HepaRG 细胞系

Animal Primary Cells/动物原代细胞

5. Mouse Cell Systems/小鼠细胞系统
5.1 Mouse Splenocytes – available from several mouse strains/小鼠脾细胞-多种小鼠类型可供选择
5.2 Mouse Peripheral Blood Mononuclear Cells – available from several mouse strains/小鼠外周血单核细胞-多种小鼠类型可供选择
5.3 Mouse CD4 T Cells – available from several mouse strains /小鼠CD4 T 细胞-多种小鼠类型可供选择
5.4 Mouse CD8 T Cells – available from several mouse strains/小鼠CD8 T 细胞-多种小鼠类型可供选择
5.5 Mouse B Cells – available from several mouse strains/小鼠B 细胞-多种小鼠类型可供选择
5.6 Mouse Bone Marrow Cells – available from several mouse strains /小鼠骨髓细胞-多种小鼠类型可供选择
5.7 Mouse Neutrophils – available from several mouse strains/小鼠中性粒细胞-多种小鼠类型可供选择
5.8 Fresh Mouse Hepatocytes/新鲜小鼠肝细胞
5.9 Frozen Mouse Hepatocytes/冻存小鼠肝细胞
5.10 Related Mouse Hepatic Cell Products/小鼠肝细胞相关产品

6. Rat Cell Systems/大鼠细胞系统
6.1 Rat Splenocytes – available from several rat strains/大鼠脾细胞-多种大鼠类型可供选择
6.2 Rat Peripheral Blood Mononuclear Cells – available from several rat strains/大鼠外周血单核细胞-多种大鼠类型可供选择
6.3 Rat CD4 T Cells – available from several rat strains/大鼠CD4 T 细胞-多种大鼠类型可供选择
6.4 Rat CD8 T Cells – available from several rat strains/大鼠CD8 T 细胞-多种大鼠类型可供选择
6.5 Rat B Cells – available from several rat strains/大鼠B 细胞-多种大鼠类型可供选择
6.6 Rat Bone Marrow Cells – available from several rat strains/大鼠骨髓细胞-多种大鼠类型可供选择
6.7 Rat Neutrophils – available from several rat strains/大鼠中性粒细胞-多种大鼠类型可供选择
6.8 Fresh Sprague Dawley Rat Hepatocytes/新鲜Sprague-Dawley 大鼠肝细胞
6.9 Frozen Sprague Dawley Rat Hepatocytes/冻存Sprague-Dawley 大鼠肝细胞
6.10 Related Sprague Dawley Rat Hepatic Cell Products/Sprague-Dawley 大鼠肝细胞相关产品
6.11 Fresh Wistar Rat Hepatocytes/新鲜Wistar 大鼠肝细胞
6.12 Frozen Wistar Rat Hepatocytes/冻存Wistar 大鼠肝细胞
6.13 Related Wistar Rat Hepatic Cell Products/Wistar 大鼠肝细胞相关产品

7. Dog Cell Systems/狗细胞系统
7.1 Fresh Beagle Dog Hepatocytes/新鲜比格犬肝细胞
7.2 Frozen Beagle Dog Hepatocytes/冻存比格犬肝细胞
7.3 Related Beagle Dog Hepatic Cell Products/比格犬肝细胞相关产品
7.4 Dog Peripheral Blood Mononuclear Cells/狗外周血单核细胞

8. Monkey Cell Systems/猴细胞系统
8.1 Fresh Cynomolgus Monkey Hepatocytes/新鲜食蟹猴肝细胞
8.2 Frozen Cynomolgus Monkey Hepatocytes/冻存食蟹猴肝细胞
8.3 Related Cynomolgus Monkey Hepatic Cell Products/食蟹猴肝细胞相关产品
8.4 Frozen Marmoset Monkey Hepatocytes/冻存绒猴肝细胞
8.5 Related Marmoset Monkey Hepatic Cell Products /绒猴肝细胞相关产品

9. Rabbit Cell Systems/兔细胞系统
9.1 Fresh Rabbit Hepatocytes/新鲜兔肝细胞
9.2 Frozen Rabbit Hepatocytes/冻存兔肝细胞
9.3 Related Rabbit Hepatic Cell Products/兔肝细胞相关产品

10. Pig Cell Systems/猪细胞系统
10.1 Fresh Minipig Hepatocytes/新鲜小型猪肝细胞
10.2 Frozen Minipig Hepatocytes/冻存小型猪肝细胞
10.3 Related Minipig Hepatic Cell Products/小型猪肝细胞相关产品

11. Guinea Pig Cell Systems/豚鼠细胞系统
11.1 Frozen Guinea Pig Hepatocytes/冻存豚鼠肝细胞
11.2 Related Guinea Pig Hepatic Cell Products/豚鼠肝细胞相关产品

12. Hamster Cell Systems/仓鼠细胞系统
12.1 Frozen Hamster Hepatocytes/冻存仓鼠肝细胞
12.2 Related Hamster Hepatic Cell Products/仓鼠肝细胞相关产品

Culture Medium and Supplements/细胞培养基及添加物

13.1 Cell Culture Medium (see also under each cell type category/细胞培养基
13.2 Cell Thawing Media/细胞解冻培养基
13.3 Cell Freezing Media/细胞冻存培养基
13.4 Cell Detach Media/细胞脱附培养基
13.5 Fetal Bovine Serum/胎牛血清
13.6 Supplements and Reagents/添加物及试剂
13.7 Balanced Salt Solutions and Water/平衡盐溶液及水

Cell Culture Model Systems/细胞培养模式系统

14.1 Human LiverbeadsTM/人肝细胞粒
14.2 Mouse LiverbeadsTM/小鼠肝细胞粒
14.3 Rat LiverbeadsTM/大鼠肝细胞粒
14.4 Dog LiverbeadsTM/狗肝细胞粒
14.5 Monkey LiverbeadsTM/猴肝细胞粒
14.6 Pig LiverbeadsTM/猪肝细胞粒
14.7 Human Hepatocyte Sandwich Culture/人肝细胞夹层胶原三明治培养基
14.8 Rat Hepatocyte Sandwich Culture/大鼠肝细胞夹层胶原三明治培养基

Tissue Engineering Systems/组织工程系统

15.1 Human Liver Slices/人肝切片
15.2 Rat Liver Slices/大鼠肝切片

Sera/血清

16.1 Normal Human Serum/正常人血清
16.2 Normal Human AB Serum/正常人AB 血清

Plasma/血浆

17.1 Normal Human Plasma/正常人血浆
17.2 Human Nickel Plasma/人镍过敏血浆

 

 

 

 

 

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