Sox2 anti-Human/Mouse Antibody说明书

ESI BIO的使命是成为客户信赖的合作伙伴,将科学发现转化为诊所。我们通过提供突破性的干细胞技术作为研究级和治疗级产品来实现这一目标,从研究到诊所的照明,以帮助我们的客户实现他们治疗疾病的目标。

ESI BIO建立在丰富的创新和科学的基础之上。我们于2000年在新加坡成立了ES Cell International,在那里我们通过制造个可翻译的临床级胚胎干细胞系来创建历史,以便分发给研究界。

 

ESI BIO现在开展业务Alameda CA. 我们是BioTime,Inc。拥有的以干细胞为基础的子公司的骄傲家族的成员,我们从中受益于的科学,知识产权和干细胞技术的深层来源。

抗体

 

胚胎干细胞(ESCs)和诱导多能干细胞(iPSCs)的表征是干细胞研究中的关键步骤。验证起始细胞群的常规过程应该简单,快速,重要的是可靠,以确保准确和一致的结果。ESI BIO为您提供关键抗体形式和活体染色工具,这些工具专为干细胞直接开发和测试而开发。

快速而可靠的多能性验证
ESI BIO的BioLite™抗体可直接染色培养液中的活细胞,因此无需牺牲可用于下游实验的有价值物质。

· 确认多能性标志物表达

· 允许在染色后继续细胞培养

· 低内毒素,不含Sodium azide的配方

· 无需固定或透化,仅需孵育30分钟

 

图1. [A]小鼠ES细胞的IF分析。用BioLite SSEA-1(Dy-Light 488)抗体染色后培养细胞和相同视野的相差图。[B] IF重组成人成纤维细胞第11天的原代iPS细胞集落的IF分析。用BioLite TRA-1-81(DyLight 488)抗体染色后培养细胞和相同视野的相差图。以10X放大率拍摄图像。

 

Sox2 anti-Human/Mouse Antibody, 100 µL

ST11001

 

 

Sox2或SRY(性别决定区Y)-box2转录因子含有高运动性组DNA结合结构域,其功能是结合并打开DNA螺旋,促进其他转录因子的作用。

Sox2或SRY(性别决定区Y)-box2转录因子含有高运动性组DNA结合结构域,其功能是结合并打开DNA螺旋,促进其他转录因子的作用。该蛋白质对于维持胚胎干(ES)细胞的自我更新和多能性是必需的,因此通常用于表征干细胞群。Sox2是神经前体细胞的重要功能标记,在神经元分化中起关键作用。

浓度 0.5毫克/毫升

物种反应性 人类,老鼠

主办 大鼠单克隆抗体

同型 IgG2a,κ

免疫原 人Sox2重组蛋白

公式 含有0.09%Sodium azide,pH7.2的磷酸盐缓冲溶液

储存和稳定性 分装并储存在-20°C。避免反复冻融循环。根据指示存放,自收到之日起6个月内稳定。

应用程序测试 免疫细胞化学/免疫荧光(ICC / IF),流式细胞仪(FC)

推荐的稀释液 流式细胞仪1:100

免疫细胞化学/免疫荧光1:100

建议针对每种应用滴定抗体以获得*性能。

替代名称 SRY(性别决定区Y)-box 2

参考 Cavallaro,M.,et al。(2008)通过来自亚形态Sox2突变体的神经干细胞减少了成熟神经元的产生。发展135:541-557。PMID:18171687。Fong

 

,H.,et al。(2008)Sox2在人胚胎干细胞中对自我更新和多能性的调节。干细胞26:1931-1938。PMID:18388306。Graham

 

,V.,et al。(2003)SOX2起到维持神经祖细胞身份的作用。Neuron 39:749-765。PMID:12948443.Takahashi

 

,K。,et al。(2007)通过确定的因子从成人人成纤维细胞诱导多能干细胞。Cell 131:861-872。PMID:18035408。

 

货号

品名

规格

品牌

ST11006

BioLite™ SSEA-1 (DyLight 488) anti-Human/Mouse Antibody, 100 µL

 100 µL

esibio

ST11008

BioLite™ TRA-1-81 (DyLight 488) anti-Human Antibody, 100 μL

 100 μL

esibio

ST11023

Nestin anti-Human Antibody, 100 μL

 100 μL

esibio

ST11003

Oct4 anti-Human/Mouse Antibody, 100 µL

 100 µL

esibio

 ST11001

Sox2 anti-Human/Mouse Antibody, 100 µL

 100 µL

esibio

ST11013

SSEA-1 anti-Human/Mouse Antibody, 100 µL

 100 µL

esibio

 ST11014

SSEA-3 anti-Human/Mouse Antibody, 100 µL

 100 µL

esibio

ST11015

SSEA-4 anti-Human Antibody, 100 µL

 100 µL

esibio

ST11018

TRA-1-60 (PE) anti-Human Antibody, 100 μL

 100 μL

esibio

ST11016

TRA-1-60 anti-Human Antibody, 100 µL

 100 µL

esibio

ST11017

TRA-1-81 anti-Human Antibody, 100 µL

 100 µL

esibio

 ES-700

ESI-017 Human Embryonic Stem Cell Line

ea

esibio

ES-701

ESI-035 Human Embryonic Stem Cell Line

ea

esibio

 ES-702

ESI-049 Human Embryonic Stem Cell Line

ea

esibio

ES-703

ESI-051 Human Embryonic Stem Cell Line

ea

esibio

ES-704

ESI-053 Human Embryonic Stem Cell Line

ea

esibio

ES-706

Human Embryonic Stem Cell Bundle

ea

esibio

 

 

上海金畔生物科技有限公司是实验试剂一站式采购服务商

1:强大的进口辐射能力,血清、抗体、耗材、大部分限制进口品等。

2:产品种类齐全,经营超过700多个品牌,基本涵盖所有生物实验试剂耗材。

3:提供加急服务,货品一般1-2周到货。

4:富有竞争力的价格优势,绝大部分价格有优势。

5:多年积累良好的信誉,大部分客户提供货到付款服务。客户包括清华、北大、交大、复旦、中山等100多所高校,ROCHE,阿斯利康、国药、fisher等药企。

6:我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech;sysy,TriLink BioTechnologies,Inc;worthington-biochem,zyagen等几十家国外公司代理。

7:我们还是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等*批发,欢迎合作。

Oct4 anti-Human/Mouse Antibody,说明书

ESI BIO的使命是成为客户信赖的合作伙伴,将科学发现转化为诊所。我们通过提供突破性的干细胞技术作为研究级和治疗级产品来实现这一目标,从研究到诊所的照明,以帮助我们的客户实现他们治疗疾病的目标。

ESI BIO建立在丰富的创新和科学的基础之上。我们于2000年在新加坡成立了ES Cell International,在那里我们通过制造个可翻译的临床级胚胎干细胞系来创建历史,以便分发给研究界。

 

ESI BIO现在开展业务Alameda CA. 我们是BioTime,Inc。拥有的以干细胞为基础的子公司的骄傲家族的成员,我们从中受益于的科学,知识产权和干细胞技术的深层来源。

抗体

 

胚胎干细胞(ESCs)和诱导多能干细胞(iPSCs)的表征是干细胞研究中的关键步骤。验证起始细胞群的常规过程应该简单,快速,重要的是可靠,以确保准确和一致的结果。ESI BIO为您提供关键抗体形式和活体染色工具,这些工具专为干细胞直接开发和测试而开发。

快速而可靠的多能性验证
ESI BIO的BioLite™抗体可直接染色培养液中的活细胞,因此无需牺牲可用于下游实验的有价值物质。

· 确认多能性标志物表达

· 允许在染色后继续细胞培养

· 低内毒素,不含Sodium azide的配方

· 无需固定或透化,仅需孵育30分钟

 

图1. [A]小鼠ES细胞的IF分析。用BioLite SSEA-1(Dy-Light 488)抗体染色后培养细胞和相同视野的相差图。[B] IF重组成人成纤维细胞第11天的原代iPS细胞集落的IF分析。用BioLite TRA-1-81(DyLight 488)抗体染色后培养细胞和相同视野的相差图。以10X放大率拍摄图像。

 

Oct4 anti-Human/Mouse Antibody, 100 µL

Oct4抗人/小鼠抗体,100μL

Oct4编码含有POU同源域(POU5F1)的转录因子,其在胚胎发育和干细胞多能性中起关键作用。

SKU: ST11003

 

Oct4编码含有POU同源域(POU5F1)的转录因子,其在胚胎发育和干细胞多能性中起关键作用。该基因在未分化的小鼠和人多能干和胚胎生殖(EG)细胞中以高水平表达,而Oct4基因表达的下调促进分化。Oct4是用于将小鼠和人成纤维细胞重编程为多能状态的关键转录因子之一,因此通常用作评估未分化的ES,iPS和EG细胞的标记物。

 

浓度 0.5毫克/毫升

物种反应性 人类,老鼠

主办 兔多克隆抗体

同型 IgG抗体

免疫原 OCT3 / 4重组蛋白片段对应于氨基酸1和360内的区域。

公式 磷酸盐缓冲溶液,1%BSA,20%甘油,pH 7和0.01%硫柳汞作为防腐剂。

储存和稳定性 分装并储存在-20°C。避免反复冻融循环。根据指示存放,自收到之日起6个月内稳定。

应用程序测试 免疫细胞化学/免疫荧光(ICC / IF),流式细胞仪(FC)

推荐的稀释液 流式细胞仪1:100

免疫细胞化学/免疫荧光1:100

建议针对每种应用滴定抗体以获得*性能。

替代名称 POU 5级同源框1,POU5F1,OTF3,OTF4

参考 Jerabek,S.,et al。(2014)OCT4:动态DNA结合干细胞多能性。Biochim Biophys Acta 1839:138-154。PMID:24145198。

 

Pesce,M.,et al。(1998)在小鼠生殖细胞分化期间Oct-4转录因子的差异表达。Mech Dev 71:89-98。PMID:9507072。

 

Takahashi,K。,et al。(2007)通过确定的因子从成人人成纤维细胞诱导多能干细胞。Cell 131:861-872。PMID:18035408。

货号

品名

规格

品牌

ST11006

BioLite™ SSEA-1 (DyLight 488) anti-Human/Mouse Antibody, 100 µL

 100 µL

esibio

ST11008

BioLite™ TRA-1-81 (DyLight 488) anti-Human Antibody, 100 μL

 100 μL

esibio

ST11023

Nestin anti-Human Antibody, 100 μL

 100 μL

esibio

ST11003

Oct4 anti-Human/Mouse Antibody, 100 µL

 100 µL

esibio

 ST11001

Sox2 anti-Human/Mouse Antibody, 100 µL

 100 µL

esibio

ST11013

SSEA-1 anti-Human/Mouse Antibody, 100 µL

 100 µL

esibio

 ST11014

SSEA-3 anti-Human/Mouse Antibody, 100 µL

 100 µL

esibio

ST11015

SSEA-4 anti-Human Antibody, 100 µL

 100 µL

esibio

ST11018

TRA-1-60 (PE) anti-Human Antibody, 100 μL

 100 μL

esibio

ST11016

TRA-1-60 anti-Human Antibody, 100 µL

 100 µL

esibio

ST11017

TRA-1-81 anti-Human Antibody, 100 µL

 100 µL

esibio

 ES-700

ESI-017 Human Embryonic Stem Cell Line

ea

esibio

ES-701

ESI-035 Human Embryonic Stem Cell Line

ea

esibio

 ES-702

ESI-049 Human Embryonic Stem Cell Line

ea

esibio

ES-703

ESI-051 Human Embryonic Stem Cell Line

ea

esibio

ES-704

ESI-053 Human Embryonic Stem Cell Line

ea

esibio

ES-706

Human Embryonic Stem Cell Bundle

ea

esibio

 

 

上海金畔生物科技有限公司是实验试剂一站式采购服务商

1:强大的进口辐射能力,血清、抗体、耗材、大部分限制进口品等。

2:产品种类齐全,经营超过700多个品牌,基本涵盖所有生物实验试剂耗材。

3:提供加急服务,货品一般1-2周到货。

4:富有竞争力的价格优势,绝大部分价格有优势。

5:多年积累良好的信誉,大部分客户提供货到付款服务。客户包括清华、北大、交大、复旦、中山等100多所高校,ROCHE,阿斯利康、国药、fisher等药企。

6:我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech;sysy,TriLink BioTechnologies,Inc;worthington-biochem,zyagen等几十家国外公司代理。

7:我们还是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等*批发,欢迎合作。

 

 

Nestin anti-Human Antibody说明书

ESI BIO的使命是成为客户信赖的合作伙伴,将科学发现转化为诊所。我们通过提供突破性的干细胞技术作为研究级和治疗级产品来实现这一目标,从研究到诊所的照明,以帮助我们的客户实现他们治疗疾病的目标。

ESI BIO建立在丰富的创新和科学的基础之上。我们于2000年在新加坡成立了ES Cell International,在那里我们通过制造个可翻译的临床级胚胎干细胞系来创建历史,以便分发给研究界。

 

ESI BIO现在开展业务Alameda CA. 我们是BioTime,Inc。拥有的以干细胞为基础的子公司的骄傲家族的成员,我们从中受益于的科学,知识产权和干细胞技术的深层来源。

抗体

 

胚胎干细胞(ESCs)和诱导多能干细胞(iPSCs)的表征是干细胞研究中的关键步骤。验证起始细胞群的常规过程应该简单,快速,重要的是可靠,以确保准确和一致的结果。ESI BIO为您提供关键抗体形式和活体染色工具,这些工具专为干细胞直接开发和测试而开发。

快速而可靠的多能性验证
ESI BIO的BioLite™抗体可直接染色培养液中的活细胞,因此无需牺牲可用于下游实验的有价值物质。

· 确认多能性标志物表达

· 允许在染色后继续细胞培养

· 低内毒素,不含Sodium azide的配方

· 无需固定或透化,仅需孵育30分钟

 

图1. [A]小鼠ES细胞的IF分析。用BioLite SSEA-1(Dy-Light 488)抗体染色后培养细胞和相同视野的相差图。[B] IF重组成人成纤维细胞第11天的原代iPS细胞集落的IF分析。用BioLite TRA-1-81(DyLight 488)抗体染色后培养细胞和相同视野的相差图。以10X放大率拍摄图像。

Nestin抗人类抗体

Nestin anti-Human Antibody, 100 μL

巢蛋白是在干细胞中表达的VI类中间丝蛋白。

SKU: ST11023

巢蛋白是在发育中枢神经系统(CNS)的干细胞中表达但在成熟CNS细胞中不表达的VI类中间丝蛋白。其表达广泛用作源自人ES和iPS细胞的神经谱系细胞的主要标记。巢蛋白也在非神经干细胞群中表达,例如胰岛祖细胞和造血祖细胞。

浓度 0.5毫克/毫升

物种反应性 人的

主办 小鼠单克隆

克隆 10C2

同型 的IgG1

免疫原 来自人巢蛋白的重组150个氨基酸片段与谷胱甘肽S转移酶缀合。

公式 含水缓冲液,0.09%Sodium azide,(pH7.2)。

储存和稳定性 储存在2-8°C。根据指示存放,自收到之日起6个月内稳定。

应用程序测试 免疫细胞化学/免疫荧光(ICC / IF)

推荐的稀释液 免疫细胞化学/免疫荧光1:100

Western Blot 1:100

建议针对每种应用滴定抗体以获得*性能。

参考 Lendahl,U。,et al。(1990)CNS干细胞表达一类新的中间丝蛋白。Cell 60(4):585-595。PMID:1689217。

 

Carpenter,MK,et al。(2001)富集人胚胎干细胞的神经元和神经前体。Exp Neurol 172(2):383-397。PMID:11716562。

 

Gilyarov,AV(2008)Nestin in central nervous system cells。Neurosci Behav Physiol 38(2):165-169。PMID:18197384。

 

 

货号

品名

规格

品牌

ST11006

BioLite™ SSEA-1 (DyLight 488) anti-Human/Mouse Antibody, 100 µL

 100 µL

esibio

ST11008

BioLite™ TRA-1-81 (DyLight 488) anti-Human Antibody, 100 μL

 100 μL

esibio

ST11023

Nestin anti-Human Antibody, 100 μL

 100 μL

esibio

ST11003

Oct4 anti-Human/Mouse Antibody, 100 µL

 100 µL

esibio

 ST11001

Sox2 anti-Human/Mouse Antibody, 100 µL

 100 µL

esibio

ST11013

SSEA-1 anti-Human/Mouse Antibody, 100 µL

 100 µL

esibio

 ST11014

SSEA-3 anti-Human/Mouse Antibody, 100 µL

 100 µL

esibio

ST11015

SSEA-4 anti-Human Antibody, 100 µL

 100 µL

esibio

ST11018

TRA-1-60 (PE) anti-Human Antibody, 100 μL

 100 μL

esibio

ST11016

TRA-1-60 anti-Human Antibody, 100 µL

 100 µL

esibio

ST11017

TRA-1-81 anti-Human Antibody, 100 µL

 100 µL

esibio

 ES-700

ESI-017 Human Embryonic Stem Cell Line

ea

esibio

ES-701

ESI-035 Human Embryonic Stem Cell Line

ea

esibio

 ES-702

ESI-049 Human Embryonic Stem Cell Line

ea

esibio

ES-703

ESI-051 Human Embryonic Stem Cell Line

ea

esibio

ES-704

ESI-053 Human Embryonic Stem Cell Line

ea

esibio

ES-706

Human Embryonic Stem Cell Bundle

ea

esibio

 

 

上海金畔生物科技有限公司是实验试剂一站式采购服务商

1:强大的进口辐射能力,血清、抗体、耗材、大部分限制进口品等。

2:产品种类齐全,经营超过700多个品牌,基本涵盖所有生物实验试剂耗材。

3:提供加急服务,货品一般1-2周到货。

4:富有竞争力的价格优势,绝大部分价格有优势。

5:多年积累良好的信誉,大部分客户提供货到付款服务。客户包括清华、北大、交大、复旦、中山等100多所高校,ROCHE,阿斯利康、国药、fisher等药企。

6:我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech;sysy,TriLink BioTechnologies,Inc;worthington-biochem,zyagen等几十家国外公司代理。

7:我们还是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等*批发,欢迎合作。

 

Phosphosolutions公司Anti-Adenylate Cyclase III-NEW产品代理


Phosphosolutions公司Anti-Adenylate Cyclase III-NEW产品代理

简要描述:公司概况

背景
基因工程– Phosphosolutions是*代可以完整描绘人体的遗传物质序列的企业。
蛋白质体学项目:Phosphosolutions是第二代试图将所有体内蛋白质表达出来的企业。
PhosphoSolutions公司—第三步我们将超越蛋白质体学 进而 专注于磷蛋白质。

详细介绍

产品咨询

 our focus 专业特色

PhosphoSolutions公司专注于蛋白质组学中的一个(10-20%)含量的小部分磷蛋白质。磷蛋白是监管控制组蛋白质的关键,这一部分是被称为phosphosome蛋白质。磷蛋白被认为是在神经系统疾病如老年痴呆症和癌症方面的关键元素,实质上,phosphosome是蛋白质组学作物的精华。

 

公司目标

简明概述:我们要成为世界上的磷蛋白组的提供者。

方案#1, 特异性磷抗体:首先我们要准备磷蛋白组。在激活或磷酸化状态下磷蛋白组是蛋白质识别研究中的*关键工具。

 

Antibodies 抗体

特异性磷抗体:Detection and quantitation of changes in the state of phosphorylation of specific proteins is of great utility in the quest to establish the function of a given protein and the consequences of its reversible phosphorylation. Two methods commonly used to measure protein phosphorylation and dephosphorylation in cell preparations employ prelabeling with 32Pi or back phosphorylation. These methods continue to be very effective and have advantages for many test systems, but they do have several practical and theoretical limitations (Nestler and Greengard, 1984). Based in large part on the successful use of short synthetic peptides to produce epitope-targeted antibodies (Lerner, 1982;Sutcliffe et al., 1983), an immunochemical approach became an attractive alternative for detecting changes in the state of phosphorylation of specific proteins at a specific site. The use of phosphorylation state-specific antibodies takes advantage of the sensitivity and selectivity afforded by immunochemical methodology, combined with relatively simple preparation and potentially broad applications.

The first report of phosphorylation-dependent antibodies appeared in 1981, when polyclonal antibodies that could detect phosphotyrosine-containing proteins were produced by immunization with benzyl phosphonate conjugated to keyhole limpet hemocyanin (KLH) (Ross et al., 1981). Shortly thereafter, Nairn and colleagues reported the production of serum antibodies that distinguished between the phospho- and dephospho-forms of G-substrate, a protein localized to cerebellar Purkinje cells and phosphorylated by cGMP-dependent protein kinase (Nairn et al., 1982). A synthetic heptapeptide, Arg-Lys-Asp-Thr-Pro-Ala-Leu, corresponding to a repeated sequence surrounding two phosphorylated threonyl residues in the intact protein, served as antigen. Rabbit antisera against a peptide-KLH conjugate were specific for the dephospho-form of G-substrate. Phospho-specific antibodies were prepared by immunization of rabbits with the purified phosphoprotein, phosphorylated in vitro to a stoichiometry of 2 mol/mol with cGMP-dependent protein kinase. Despite this initial success, other attempts in our laboratory to produce phospho-specific polyclonal antisera by immunization with the phospho-form of intact proteins were not very successful, probably because of two significant factors. First, many phosphorylated proteins are believed to undergo rapid dephosphorylation during immunization, regardless of the route of injection, leading to the loss of the desired phospho-epitope. Second, holoproteins generally contain multiple immunogenic epitopes; this decreases the probability that colonal dominance for a phospho-specific epitope will be obtained.

Taking a more direct approach utilizing phosphorylated and unphosphorylated forms of synthetic phosphopeptides, we developed a general protocol for the production of phosphorylation state-specific antibodies for substrates with established site(s) of phosphorylation (Czernik et al., 1991)). In early stages of our development of this methodology, phosphopeptides were routinely prepared by enzymatic phosphorylation (Czernik et al., 1991). Although this approach remains perfectly valid today, the preparation of synthetic phosphopeptides using Fmoc derivatives of phosphoamino acids has become the state-of-the-art (Czernik et al., 1995;Czernik et al., 1996). Likewise, we have examined the use of both polyclonal and monoclonal techniques for antibody production. Given the high success rate that we and others have obtained with the polyclonal technique, it has become the method of choice, because it is an easier and less costly method for the average laboratory. However, when appropriate, this approach can be readily adapted for monoclonal antibody production.

参考文献

1. Czernik AJ, Girault J-A, Nairn AC, Chen J, Snyder G, Kebabian J, Greengard P (1991) Production of phosphorylation state-specific antibodies. Methods Enzymol 201: 264-283.

2. Czernik AJ, Mathers J, Mische SM (1997) Phosphorylation state-specific antibodies. Neuromethods: Regulatory Protein Modification: Techniques & Protocols 30: 219-250.

3. Czernik AJ, Mathers J, Tsou K, Greengard P, Mische SM (1995) Phosphorylation state-specific antibodies: preparation and applications. Neuroprotocols 6: 56-61.

4. Lerner, R. A. Tapping the immunological repertoire to produce antibodies of predetermined specificity. Nature 299, 593-596. 1982.

5. Nairn AC, Detre JA, Casnellie JE, Greengard P (1982) Serum antibodies that distinguish between the phospho- and dephospho-forms of a phosphoprotein. Nature (Lond ) 299: 734-736.

6. Nestler, E. J. and Greengard, P. Protein Phosphorylation in the Nervous System. Nestler and Greengard. Protein Phosphorylation in the Nervous System. [8], 255-299. 1984. New York, Wiley. 

8. Sutcliffe JG, Shinnick TM, Green N, Lerner RA (1983) Antibodies that react with predetermined sites on proteins. Science 219: 660-666.

主营产品清单如下:

Item: Anti-Adenylate Cyclase III-NEW!
Category:  
Sub-Category:  
SKU/Catalog Number: 85-AC3
Datasheet:  click to view
SKU Price Formulation Applications Amount Qty
85-AC3 $325.00 affinity purified polyclonal antibody WB, IF 100 ul

 

Phosphosolutions公司Anti-Actin产品代理


Phosphosolutions公司Anti-Actin产品代理

简要描述:Phosphosolutions公司Anti-Actin产品代理

详细介绍

产品咨询

 公司概况

 
背景
基因工程– Phosphosolutions是*代可以完整描绘人体的遗传物质序列的企业。
蛋白质体学项目:Phosphosolutions是第二代试图将所有体内蛋白质表达出来的企业。
PhosphoSolutions公司—第三步我们将超越蛋白质体学 进而 专注于磷蛋白质。
 
our focus 专业特色
PhosphoSolutions公司专注于蛋白质组学中的一个(10-20%)含量的小部分磷蛋白质。磷蛋白是监管控制组蛋白质的关键,这一部分是被称为phosphosome蛋白质。磷蛋白被认为是在神经系统疾病如老年痴呆症和癌症方面的关键元素,实质上,phosphosome是蛋白质组学作物的精华。
 
公司目标
简明概述:我们要成为世界上的磷蛋白组的提供者。
方案#1, 特异性磷抗体:首先我们要准备磷蛋白组。在激活或磷酸化状态下磷蛋白组是蛋白质识别研究中的*关键工具。
 
Antibodies 抗体
特异性磷抗体:Detection and quantitation of changes in the state of phosphorylation of specific proteins is of great utility in the quest to establish the function of a given protein and the consequences of its reversible phosphorylation. Two methods commonly used to measure protein phosphorylation and dephosphorylation in cell preparations employ prelabeling with 32Pi or back phosphorylation. These methods continue to be very effective and have advantages for many test systems, but they do have several practical and theoretical limitations (Nestler and Greengard, 1984). Based in large part on the successful use of short synthetic peptides to produce epitope-targeted antibodies (Lerner, 1982;Sutcliffe et al., 1983), an immunochemical approach became an attractive alternative for detecting changes in the state of phosphorylation of specific proteins at a specific site. The use of phosphorylation state-specific antibodies takes advantage of the sensitivity and selectivity afforded by immunochemical methodology, combined with relatively simple preparation and potentially broad applications.
The first report of phosphorylation-dependent antibodies appeared in 1981, when polyclonal antibodies that could detect phosphotyrosine-containing proteins were produced by immunization with benzyl phosphonate conjugated to keyhole limpet hemocyanin (KLH) (Ross et al., 1981). Shortly thereafter, Nairn and colleagues reported the production of serum antibodies that distinguished between the phospho- and dephospho-forms of G-substrate, a protein localized to cerebellar Purkinje cells and phosphorylated by cGMP-dependent protein kinase (Nairn et al., 1982). A synthetic heptapeptide, Arg-Lys-Asp-Thr-Pro-Ala-Leu, corresponding to a repeated sequence surrounding two phosphorylated threonyl residues in the intact protein, served as antigen. Rabbit antisera against a peptide-KLH conjugate were specific for the dephospho-form of G-substrate. Phospho-specific antibodies were prepared by immunization of rabbits with the purified phosphoprotein, phosphorylated in vitro to a stoichiometry of 2 mol/mol with cGMP-dependent protein kinase. Despite this initial success, other attempts in our laboratory to produce phospho-specific polyclonal antisera by immunization with the phospho-form of intact proteins were not very successful, probably because of two significant factors. First, many phosphorylated proteins are believed to undergo rapid dephosphorylation during immunization, regardless of the route of injection, leading to the loss of the desired phospho-epitope. Second, holoproteins generally contain multiple immunogenic epitopes; this decreases the probability that colonal dominance for a phospho-specific epitope will be obtained.
Taking a more direct approach utilizing phosphorylated and unphosphorylated forms of synthetic phosphopeptides, we developed a general protocol for the production of phosphorylation state-specific antibodies for substrates with established site(s) of phosphorylation (Czernik et al., 1991)). In early stages of our development of this methodology, phosphopeptides were routinely prepared by enzymatic phosphorylation (Czernik et al., 1991). Although this approach remains perfectly valid today, the preparation of synthetic phosphopeptides using Fmoc derivatives of phosphoamino acids has become the state-of-the-art (Czernik et al., 1995;Czernik et al., 1996). Likewise, we have examined the use of both polyclonal and monoclonal techniques for antibody production. Given the high success rate that we and others have obtained with the polyclonal technique, it has become the method of choice, because it is an easier and less costly method for the average laboratory. However, when appropriate, this approach can be readily adapted for monoclonal antibody production.
参考文献
1. Czernik AJ, Girault J-A, Nairn AC, Chen J, Snyder G, Kebabian J, Greengard P (1991) Production of phosphorylation state-specific antibodies. Methods Enzymol 201: 264-283.
2. Czernik AJ, Mathers J, Mische SM (1997) Phosphorylation state-specific antibodies. Neuromethods: Regulatory Protein Modification: Techniques & Protocols 30: 219-250.
3. Czernik AJ, Mathers J, Tsou K, Greengard P, Mische SM (1995) Phosphorylation state-specific antibodies: preparation and applications. Neuroprotocols 6: 56-61.
4. Lerner, R. A. Tapping the immunological repertoire to produce antibodies of predetermined specificity. Nature 299, 593-596. 1982.
5. Nairn AC, Detre JA, Casnellie JE, Greengard P (1982) Serum antibodies that distinguish between the phospho- and dephospho-forms of a phosphoprotein. Nature (Lond ) 299: 734-736.
6. Nestler, E. J. and Greengard, P. Protein Phosphorylation in the Nervous System. Nestler and Greengard. Protein Phosphorylation in the Nervous System. [8], 255-299. 1984. New York, Wiley. 
8. Sutcliffe JG, Shinnick TM, Green N, Lerner RA (1983) Antibodies that react with predetermined sites on proteins. Science 219: 660-666.
主营产品清单如下:
Item: Anti-Actin
Category:  
Sub-Category:  
SKU/Catalog Number: 125-ACT
Datasheet:  click to view
SKU Price Formulation Application Amount Qty
125-ACT $275.00 ascites fluid WB, IF, IHC 100 ul