Dianova公司为德国的病理免疫组化产品供应商，具备先进的抗体制备工艺及严格的质控系统，产品在上享有良好的声誉。
Dianova DIA-900-BIOT说明书
DIA-900-BIOT – Anti-HIS Epitope-Tag (rec) from Mouse (Clone: 13/45/31) – Biotin – 100 µg
DIA-900-BIOT – 来自小鼠的抗HIS表位标签（rec）（克隆：13/45/31） – 生物素 – 100μg
Art.-Nr.
DIA-900-BIOT
特异性
他的Epitop-Tag
物种反应性
全部
重组
寄主物种
老鼠
同型
的IgG1
克隆
13/45/31
克隆性（单克隆/多克隆）
单克隆
应用
免疫组化（冷冻切片）
免疫荧光
流式细胞仪
Western Blot
免疫组织化学（IHC）
共轭
生物素
产品系列/主题
表位 – 标签，Haptens和标签
有可能的使用
仅供研究使用
温度 – 储存
2-8℃
搜索代码
DIA935
制造商/品牌
dianova
反应
小鼠单克隆抗（His）6-标签抗体，克隆13/45 / 31-2（H.Zentgraf / DKFZ Heidelberg，Germany），特异性地检测细胞和复杂细胞裂解物中的任何类型的组氨酸标记的蛋白质。具有3x 10-10M（Biacore TM分析）的值，抗体的亲和力是显着的。该单克隆抗体与含有至少6个组氨酸残基的表位的重组蛋白特异性反应，所述表位位于N末端，C末端或位于两个终点。更高数量的组氨酸残基导致抗体的结合亲和力增加，例如具有10个组氨酸残基的表达产物使亲和力增加约10至20倍。在大多数情况下，抗体结合不需要额外的侧翼氨基酸，
背景
重组蛋白质用于分子生物学中的各种目的，例如用于研究蛋白质 – 核酸或蛋白质 – 蛋白质相互作用机制的抗体的产生。已经建立了许多原核表达载体，使得能够合成目的蛋白质作为与肽的融合，从而促进纯化。重组蛋白在大肠杆菌中作为与邻近组氨酸残基的融合蛋白的表达是流行的方法之一，因为蛋白质具有有用的属性。组氨酸 – 标签基序通过螯合作用对Ni2 +的亲和力强且足够选择性，使得能够通过在Ni2 + -NTA吸附剂上的亲和色谱法将蛋白质纯化至均一。
检测（HIS）6-p53 Hela细胞裂解物（根据标准程序用HIS标记的蛋白质载体转染，SDS-PAGE和蛋白质印迹）。灵敏度：在1：100抗体稀释度下对8μg总蛋白质中的1ng蛋白质进行特异性检测/对于较不敏感的检测，抗体分析高达1：10.000（约6ng HIS标记的蛋白质）。
参考文献：
克隆13/45/31是世界上引用多的His-Tag抗体之一。
发表：
1.Zentgraf H，Frey M，Schwinn S，Tessmer C，Willemann B，Samstag Y，Velhagen I.通过使用高特异性小鼠单克隆抗组氨酸标签抗体检测组氨酸标记的融合蛋白。Nucleic Acids Res.16,3347-8,1995。
Dianova2019年产品价格表
|
货号 |
品名 |
规格 |
价格 |
品牌 |
|
DIA-100 |
Anti-Fibroblasten (CD90) (Hu) from Mouse (Clone: AS02) |
200µg |
4680 |
dianova |
|
DIA-100-M |
Anti-Fibroblasten (CD90) (Hu) from Mouse (Clone: AS02) |
20µg |
0 |
dianova |
|
DIA-120 |
Anti-Fibroblasten (CD90) (Hu) from Mouse (Clone: AS02) -FITC |
100test |
5360 |
dianova |
|
DIA-530-P05 |
Anti-p53 (Hu) from Mouse (Clone: CC53) |
0.5ml |
4780 |
dianova |
|
DIA-530-M |
Anti-p53 (Hu) from Mouse (Clone: CC53) |
0.1ml |
0 |
dianova |
|
DIA-670-P05 |
Anti-Ki-67/MIB1 (Hu) from Mouse (Clone: Ki-67P) CE-IVD |
0.5ml |
4780 |
dianova |
|
DIA-670-P1 |
Anti-Ki-67/MIB1 (Hu) from Mouse (Clone: Ki-67P) CE-IVD |
1ml |
6500 |
dianova |
|
DIA-670-M |
Anti-Ki-67/MIB1 (Hu) from Mouse (Clone: Ki-67P) CE-IVD |
0.1ml |
0 |
dianova |
|
DIA-700-P05 |
Anti-GFAP (Hu) from Mouse (Clone: IF3) CE-IVD |
0.5ml |
4780 |
dianova |
|
DIA-700-M |
Anti-GFAP (Hu) from Mouse (Clone: IF3) CE-IVD |
0.5ml |
0 |
dianova |
|
DIA-AX1 |
Anti-ATRX (Hu) from Mouse (Clone: AX1) |
0.5ml |
6760 |
dianova |
|
DIA-CAL-100 |
Anti-CALRETICULIN mutation (Hu) from Mouse (Clone CAL2) |
0.1ml |
5960 |
dianova |
|
DIA-CAL-250 |
Anti-CALRETICULIN mutation (Hu) from Mouse (Clone CAL2) |
0.25ml |
13960 |
dianova |
|
DIA-H09 |
Anti-IDH1 R132H (Hu) from Mouse (Clone: H09) CE-IVD |
0.5ml |
9640 |
dianova |
|
DIA-H09-M |
Anti-IDH1 R132H (Hu) from Mouse (Clone: H09) CE-IVD |
0.1ml |
2840 |
dianova |
|
DIA-H09-L |
Anti-IDH1 R132H (Hu) from Mouse (Clone: H09), prediluted, ready-to-use |
8ml |
9640 |
dianova |
|
DIA-W09 |
Anti-IDH1 (Hu) from Rat (Clone: W09) |
0.5ml |
4960 |
dianova |
|
DIA-TG1 |
Anti-TIGIT (Hu) from Mouse (Clone TG1) |
0.5ml |
20400 |
dianova |
|
DIA-TG1-M |
Anti-TIGIT (Hu) from Mouse (Clone TG1) |
0.1ml |
5720 |
dianova |
|
DIA-TC8 |
Anti-CD8 (Hu) from Mouse (Clone TC8 ) |
0.5ml |
9200 |
dianova |
|
DIA-P16-OD |
Anti-P16 (Hu) from Mouse (Clone JAP16) |
0.1ml |
7360 |
dianova |
|
DIA-303 |
Anti-CD3 (Ms, FFPE) from Rat (Clone: HH3E) |
0.5ml |
6900 |
dianova |
|
DIA-310 |
Anti-CD31 (Ms, FFPE) from Rat (Clone: SZ31) |
0.5ml |
8040 |
dianova |
|
DIA-404 |
Anti-CD4 (Ms, FFPE) from Rat (Clone GHH8) |
0.2ml |
6900 |
dianova |
|
DIA-808 |
Anti-CD8a (Ms, FFPE) from Rat (Clone GHH8) |
0.2ml |
6900 |
dianova |
|
DIA-60 |
Anti-FasL, soluble (Hu) from Mouse (Clone: 94) |
1ml |
4760 |
dianova |
|
DIA-900-100 |
Anti-HIS-Tag (affinity 3x 10‐10M (Biacore™) from Mouse (Clone: 13/45/31) |
100µg |
2960 |
dianova |
|
DIA-900-200 |
Anti-HIS Epitope-Tag (affinity 3x 10‐10M (Biacore™) from Mouse (Clone: 13/45/31) |
200µg |
5160 |
dianova |
|
DIA-900-FITC |
Anti-HIS Epitope-Tag (affinity 3x 10‐10M (Biacore™) from Mouse (Clone:13/45/31) -FITC |
1ml |
5960 |
dianova |
|
DIA-900-BIOT |
Anti-HIS Epitope-Tag (affinity 3x 10‐10M (Biacore™) from Mouse (Clone:13/45/31) -Biotin |
100µg |
3960 |
dianova |
|
DIA-910-200-ABF |
Anti-HIS Epitope-Tag (affinity 3x 10‐10M (Biacore™) from Mouse (Clone:13/45/31) Acid/BSA-free |
200µg |
4960 |
dianova |
|
DIA.910-1MG-ABF |
Anti-HIS Epitope-Tag (affinity 3x 10‐10M (Biacore™) from Mouse (Clone:13/45/31) Acid/BSA-free |
1mg |
19840 |
dianova |
|
SCR-028841 |
IS Adhesion Slides |
50pcs. |
1620 |
dianova |
|
SCR-028855 |
IS Adhesion Slides |
5pcs. |
询价 |
dianova |
|
SCR-028842 |
IS Adhesion Slides |
100pcs. |
2440 |
dianova |
|
SCR-038447 |
Immunoselect Antifading Mounting Medium |
15ml |
960 |
dianova |
|
SCR-038587 |
Immunoselect Antifading Mounting Medium |
1ml |
询价 |
dianova |
|
SCR-038448 |
Immunoselect Antifading Mounting Medium DAPI |
15ml |
1180 |
dianova |
|
SCR-038588 |
Immunoselect Antifading Mounting Medium DAPI |
1ml |
询价 |
dianova |
|
SCR-038449 |
Immunoselect Antifading Mounting Medium PI |
15ml |
1180 |
dianova |
|
SCR-072967 |
Immunoselect Mounting Medium without hardener |
15ml |
960 |
dianova |
|
SCR-093035 |
Immunoselect Antifading Mounting Medium DAPI without hardener |
15ml |
1180 |
dianova |
|
SCR-093036 |
Immunoselect Antifading Mounting Medium PI without hardener |
15ml |
1180 |
dianova |
|
DIA-0302-I |
p53-auto antibody ELISA-Kit (Human) CE-IVD |
96test |
10960 |
dianova |
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