上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
Bst 2.0 DNA Polymerase displays improved amplification speed, yield and salt tolerance
Interested in glycerol-free or custom formulations? – contact our Custom Solutions Group
Bulk packaging may also be available and requested for large recurring orders.
Learn More
产品信息
Bst 2.0 DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment (Bst DNA Polymerase, Large Fragment). Bst 2.0 DNA Polymerase contains 5´→3´ DNA polymerase activity and strong strand displacement activity but lacks 5´→3´ exonuclease activity. Bst 2.0 DNA Polymerase displays improved amplification speed, yield, salt tolerance and thermostability compared to wild-type Bst DNA Polymerase, Large Fragment.
Bst 2.0 DNA Polymerase is prepared from an E. coli strain that expresses the Bst 2.0 DNA Polymerase protein from an inducible promoter.
Isothermal Amplification
本产品提供以下试剂或组分：
| NEB # | 名称 | 组分货号 | 储存温度 | 数量 | 浓度 | |||||||||||||||||||||||||
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One unit is defined as the amount of enzyme that will incorporate 25 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.
1X Isothermal Amplification Buffer Pack
Incubate at 65°C
1X Isothermal Amplification Buffer Pack
20 mM Tris-HCl
10 mM (NH4)2SO4
50 mM KCl
2 mM MgSO4
0.1% Tween® 20
(pH 8.8 @ 25°C)
10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.1% Triton® X-100
pH 7.1 @ 25°C
80°C for 20 min
50 mM KCl, 20 mM Tris- HCl (pH 8.8), 10 mM MgCl2, 30 nM M13mp18 SS DNA, 70 nM M13 sequencing primer (–47) 24 mer, 200 μM dATP, 200 μM dCTP, 200 μM dGTP, 100 μM dTTP including [3H]-dTTP and 100 μg/ml BSA.
操作说明、说明书 & 用法
工具 & 资源
FAQs & 问题解决指南
引用 & 技术文献
Powered by Bioz See more details on Bioz质控、安全 & 法规
产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
Bst 2.0 DNA Polymerase displays improved amplification speed, yield and salt tolerance
Interested in glycerol-free or custom formulations? – contact our Custom Solutions Group
Bulk packaging may also be available and requested for large recurring orders.
Learn More
产品信息
Bst 2.0 DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment (Bst DNA Polymerase, Large Fragment). Bst 2.0 DNA Polymerase contains 5´→3´ DNA polymerase activity and strong strand displacement activity but lacks 5´→3´ exonuclease activity. Bst 2.0 DNA Polymerase displays improved amplification speed, yield, salt tolerance and thermostability compared to wild-type Bst DNA Polymerase, Large Fragment.
Bst 2.0 DNA Polymerase is prepared from an E. coli strain that expresses the Bst 2.0 DNA Polymerase protein from an inducible promoter.
Isothermal Amplification
本产品提供以下试剂或组分：
| NEB # | 名称 | 组分货号 | 储存温度 | 数量 | 浓度 | |||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
||||||||||||||||||||||||||||||
|
||||||||||||||||||||||||||||||
|
||||||||||||||||||||||||||||||
One unit is defined as the amount of enzyme that will incorporate 25 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.
1X Isothermal Amplification Buffer Pack
Incubate at 65°C
1X Isothermal Amplification Buffer Pack
20 mM Tris-HCl
10 mM (NH4)2SO4
50 mM KCl
2 mM MgSO4
0.1% Tween® 20
(pH 8.8 @ 25°C)
10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.1% Triton® X-100
pH 7.1 @ 25°C
80°C for 20 min
50 mM KCl, 20 mM Tris- HCl (pH 8.8), 10 mM MgCl2, 30 nM M13mp18 SS DNA, 70 nM M13 sequencing primer (–47) 24 mer, 200 μM dATP, 200 μM dCTP, 200 μM dGTP, 100 μM dTTP including [3H]-dTTP and 100 μg/ml BSA.
操作说明、说明书 & 用法
工具 & 资源
FAQs & 问题解决指南
引用 & 技术文献
Powered by Bioz See more details on Bioz质控、安全 & 法规
产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
Bst 3.0 DNA Polymerase demonstrates robust performance even in high concentrations of amplification inhibitors and features significantly increased reverse transcriptase activity compared to Bst DNA Polymerase.
Interested in glycerol-free or custom formulations? – contact our Custom Solutions Group
Bulk packaging may also be available and requested for large recurring orders.
Learn More
产品信息
Bst 3.0 DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment engineered and fused to a novel nucleic acid binding domain for improved isothermal amplification performance and increased reverse transcription activity. Bst 3.0 DNA Polymerase contains 5´→3´ DNA polymerase activity with either DNA or RNA templates and strong strand displacement activity, but lacks 5´→3´ and 3´→5´ exonuclease activity. Bst 3.0 DNA Polymerase demonstrates robust performance even in high concentrations of amplification inhibitors, including dUTP and features significantly increased reverse transcriptase activity compared to Bst DNA Polymerase.
An example of the utility and speed offered by Bst 3.0 DNA Polymerase can be seen in this publication that explores the development of a novel digital LAMP assay: Rapid pathogen-specific phenotypic antibiotic susceptibility testing using digital LAMP quantification in clinical samples.
An E. coli strain that carries the engineered Bst 3.0 gene.
本产品提供以下试剂或组分：
| NEB # | 名称 | 组分货号 | 储存温度 | 数量 | 浓度 | |||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
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|
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|
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One unit is defined as the amount of enzyme that will incorporate 25 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.
1X Isothermal Amplification Buffer II Pack
Incubate at 65°C
1X Isothermal Amplification Buffer II Pack
20 mM Tris-HCl
10 mM (NH4)2SO4
150 mM KCl
2 mM MgSO4
0.1% Tween® 20
(pH 8.8 @ 25°C)
rCutSmart™ Buffer: 100%
NEBuffer™ r1.1: 10%
NEBuffer™ r2.1: 100%
NEBuffer™ r3.1: 100%
100 mM KCl
10 mM Tris-HCl
0.1 mM EDTA
1 mM DTT
0.1% Triton® X-100
50% Glycerol
pH 7.4 @ 25°C
80°C for 5 min
50 mM KCl, 20 mM Tris- HCl (pH 8.8), 10 mM MgCl2, 30 nM M13mp18 SS DNA, 70 nM M13 sequencing primer (–47) 24 mer, 200 μM dATP, 200 μM dCTP, 200 μM dGTP, 100 μM dTTP including [3H]-dTTP and 100 μg/ml BSA.
操作说明、说明书 & 用法
工具 & 资源
FAQs & 问题解决指南
引用 & 技术文献
Powered by Bioz See more details on Bioz质控、安全 & 法规
产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
Bst 3.0 DNA Polymerase demonstrates robust performance even in high concentrations of amplification inhibitors and features significantly increased reverse transcriptase activity compared to Bst DNA Polymerase.
Interested in glycerol-free or custom formulations? – contact our Custom Solutions Group
Bulk packaging may also be available and requested for large recurring orders.
Learn More
产品信息
Bst 3.0 DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment engineered and fused to a novel nucleic acid binding domain for improved isothermal amplification performance and increased reverse transcription activity. Bst 3.0 DNA Polymerase contains 5´→3´ DNA polymerase activity with either DNA or RNA templates and strong strand displacement activity, but lacks 5´→3´ and 3´→5´ exonuclease activity. Bst 3.0 DNA Polymerase demonstrates robust performance even in high concentrations of amplification inhibitors, including dUTP and features significantly increased reverse transcriptase activity compared to Bst DNA Polymerase.
An example of the utility and speed offered by Bst 3.0 DNA Polymerase can be seen in this publication that explores the development of a novel digital LAMP assay: Rapid pathogen-specific phenotypic antibiotic susceptibility testing using digital LAMP quantification in clinical samples.
An E. coli strain that carries the engineered Bst 3.0 gene.
本产品提供以下试剂或组分：
| NEB # | 名称 | 组分货号 | 储存温度 | 数量 | 浓度 | |||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
||||||||||||||||||||||||||||||
|
||||||||||||||||||||||||||||||
|
||||||||||||||||||||||||||||||
One unit is defined as the amount of enzyme that will incorporate 25 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.
1X Isothermal Amplification Buffer II Pack
Incubate at 65°C
1X Isothermal Amplification Buffer II Pack
20 mM Tris-HCl
10 mM (NH4)2SO4
150 mM KCl
2 mM MgSO4
0.1% Tween® 20
(pH 8.8 @ 25°C)
rCutSmart™ Buffer: 100%
NEBuffer™ r1.1: 10%
NEBuffer™ r2.1: 100%
NEBuffer™ r3.1: 100%
100 mM KCl
10 mM Tris-HCl
0.1 mM EDTA
1 mM DTT
0.1% Triton® X-100
50% Glycerol
pH 7.4 @ 25°C
80°C for 5 min
50 mM KCl, 20 mM Tris- HCl (pH 8.8), 10 mM MgCl2, 30 nM M13mp18 SS DNA, 70 nM M13 sequencing primer (–47) 24 mer, 200 μM dATP, 200 μM dCTP, 200 μM dGTP, 100 μM dTTP including [3H]-dTTP and 100 μg/ml BSA.
操作说明、说明书 & 用法
工具 & 资源
FAQs & 问题解决指南
引用 & 技术文献
Powered by Bioz See more details on Bioz质控、安全 & 法规
产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]
Bst DNA 聚合酶，大片段，Bst DNA Polymerase, Large Fragment，货号-规格：CW2713S-800 U
| 市场价： | ￥198.0 | |
| 价格： |
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| 品牌： | 康为世纪 | |
| 规格： | 800 U |
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产品详情
说明书下载
参考文献
Bst DNA 聚合酶，大片段 Bst DNA Polymerase, Large Fragment 产品货号：CW2713S 产品规格：800 U Bst DNA 聚合酶，大片段 Bst DNA Polymerase, Large Fragment是通过大肠杆菌表达纯化的重组酶，其基因来源于Bacillus stearothermophilus，并在原始序列的基础上进行了部分点突变。该蛋白具有5'→3' DNA聚合酶活性，无5'→3'和3'→5'核酸外切酶活性，具有强链置换活性。应用于DNA等温扩增（LAMP）、多重置换扩增（MDA）、全基因组扩增（WGA）、建库测序等。 Bst DNA Polymerase, Large Fragment 立即下载 |