This medium pack includes 12 grams of Yeast Carbon Base Medium Powder and 10 ml of Acetamide Solution.

The Yeast Carbon Base Medium Powder contains sufficient reagents needed to make 1 liter of Yeast Carbon Base (YCB) Agar Medium containing 5 mM acetamide. 

YCB Agar Medium is used for acetamide selection of K. lactis cells that have been transformed with a pKLAC1-based expression vector (NEB #N3740). The medium contains glucose and all nutrients needed to sustain growth of K. lactis GG799 cells except a simple nitrogen source. Only transformed cells can utilize acetamide provided in the medium as a source of nitrogen after it is broken down to ammonia by acetamidase (the product of the amdS gene present in pKLAC1).

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