上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
Chitin Magnetic Beads
An affinity matrix for the small-scale isolation of target proteins fused to a chitin binding domain (CBD).
- Chitin beads have been prepared having a magnetite core, thereby permitting the magnetic isolation of CBD-fusion proteins from cell culture supernatants
- The matrix can be regenerated without loss of binding capacity
- Immobilized fusion proteins can be used in subsequent experiments to capture (pull down) target proteins from crude cell lysates that interact with the immobilized fusion protein
- Binding capacity of 2 mg chitin binding domain protein/ml bed volume
- Quick, small-scale purification of CBD-fusion proteins enables higher throughput and efficiency
- Removal of CBD-tag during elution typically yields highly pure, native protein
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Chitin Resin |NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
Chitin Resin
An affinity matrix for the isolation of target proteins fused to an intein-chitin binding domain fusion
- Strong specific binding for CBD-fusion protein affords highly pure protein of interest from crude lysate in one step
- Removal of CBD-tag during elution typically yields highly pure, native protein without the use of a protease
- This resin, when used with pTXB1, allows for the isolation of native recombinant proteins possessing a reactive C-terminal thioester that can be used for applications in intein-mediated protein ligation (IPL) and site-specific labeling
- Binding capacity: 2 mg maltose-binding protein/mL bed volume released from the resin after cleavage of the maltose-binding-CBD-fusion protein
- Resin may be regenerated up to 5 times
