Capricorn Scientific CHO-A说明书

Capricorn Scientific成立于2013年,是一家新的德国细胞培养基,血清和试剂生产商。我们生产的主要重点是生产用于生物技术,诊断和研究应用的高质量动物血清。严格的测试程序以及全面的可追溯性记录和文档保证了我们产品的和可重复的质量。

作为一家规模较小的公司,我们可以灵活地提供各种传统和定制产品,以更低的成本,更短的交付周期和个性化的客户服务满足当今市场不断增长的需求。

我们的技术,经验丰富的专家团队多年来一直致力于细胞培养领域。我们致力于为活跃的细胞培养公司提供高质量,可靠性和服务。

 

Capricorn Scientific CHO-A说明书

CP-CHO Medium, protein-free CHO Medium, with L-Glutamine, sterile-filtered

CP-CHO培养基,无蛋白CHO培养基,含L-谷氨酰胺,无菌过滤

 

号:CHO-A(500毫升)

 

产品阐述,产品说明

 

CP-CHO是在无血清条件下培养CHO细胞的培养基.该培养基用于支持灌注培养的生长。

 

一些成分,如酵母抽提物的添加,以丰富的介质,以获得大的性能。

 

培养基不含动物成分。

 

Applications ·

 

CHO K1和CHO-DG-44细胞

 

·在CHO细胞灌注培养中的应用

 

商品概述

 

外观(颜色)清澈的液体

 

pH 6.9 – 7.5

 

渗透压290-340 mOsm/kg

 

内毒素≤10 EU/ml

 

不育通行证

 

储存温度2-8度;黑暗和凉爽

 

保质期指到期日的产品标签

 

使用建议

 

可以建议增加:

 

Pluronic:1克/升

 

柠檬酸铁:20毫克/升

 

胰岛素:5毫克/升

 

配方

 

CP-CHO的配方是摩羯座科学公司的专有技术。如需更多资料,请参阅我们的技术服务。

 

预防措施及免责声明

 

本产品仅供研究用。请查阅材料安全数据表,以获取有关危险和安全处理做法的信息。

 

 

Capricorn Scientific产品目录:

货号

品名

规格

价格

DMEM-LPA

DMEM Low Glucose (1 g/l), with L-Glutamine, with Sodium Pyruvate

500 ml

7.50 €

DMEM-LPSTA

DMEM Low Glucose (1 g/l), with Stable Glutamine, with Sodium Pyruvate

500 ml

9.50 €

DMEM-LPXA

DMEM Low Glucose (1 g/l), w/o L-Glutamine, with Sodium Pyruvate

500 ml

7.50 €

DMEM-HA

DMEM High Glucose (4.5 g/l), with L-Glutamine

500 ml

6.40 €

DMEM-HHSTA

DMEM High Glucose (4.5 g/l), with Stable Glutamine, with 25 mM HEPES

500 ml

9.50 €

DMEM-HPA

DMEM High Glucose (4.5 g/l), with L-Glutamine, with Sodium Pyruvate

500 ml

7.50 €

DMEM-HPSTA

DMEM High Glucose (4.5 g/l), with Stable Glutamine, with Sodium Pyruvate

500 ml

7.00 €

DMEM-HPXA

DMEM High Glucose (4.5 g/l), w/o L-Glutamine, with Sodium Pyruvate

500 ml

7.50 €

DMEM-HXA

DMEM High Glucose (4.5 g/l), w/o L-Glutamine

500 ml

6.40 €

DMEM-HXRXA

DMEM High Glucose (4.5 g/l), w/o L-Glutamine, w/o Phenol Red

500 ml

10.50 €

DMEM-12-A

DMEM/Ham’s F-12, with L-Glutamine

500 ml

8.60 €

DMEM-12-HXA

DMEM/Ham’s F-12, w/o L-Glutamine, with 15 mM HEPES

500 ml

8.60 €

HAM-12-A

Ham’s F-12, with L-Glutamine

500 ml

9.50 €

IMDM-A

IMDM, with L-Glutamine, without Supplements

500 ml

9.00 €

L15-XA

Leibovitz’s L-15 Medium, w/o L-Glutamine

500 ml

12.40 €

MCC-A

McCoy’s Medium, with L-Glutamine

500 ml

12.70 €

M199-A

Medium 199, with L-Glutamine

500 ml

8.90 €

MEM-A

MEM with Earle’s Salts, with L-Glutamine

500 ml

7.20 €

MEM-STA

MEM with Earle’s Salts, with Stable Glutamine

500 ml

9.40 €

MEM-XA

MEM with Earle’s Salts, w/o L-Glutamine

500 ml

6.40 €

MEMA-RXA

MEM Alpha Modification, w/o L-Glutamine, with Nucleosides

500 ml

19.90 €

MEMA-XRXA

MEM Alpha Modification, w/o L-Glutamine, w/o Nucleosides

500 ml

13.00 €

RPMI-A

RPMI 1640, with L-Glutamine

500 ml

6.40 €

RPMI-HA

RPMI 1640, with L-Glutamine, with 25 mM HEPES

500 ml

9.90 €

RPMI-HXA

RPMI 1640, w/o L-Glutamine, with 25 mM HEPES

500 ml

9.50 €

RPMI-STA

RPMI 1640, with Stable Glutamine

500 ml

8.40 €

RPMI-XA

RPMI 1640, w/o L-Glutamine

500 ml

5.80 €

RPMI-XRXA

RPMI 1640, w/o L-Glutamine, w/o Phenol Red

500 ml

9.90 €

FM1-F

FreezeMe One, Cryopreservation Medium with Fetal Bovine Serum

50 ml

65.00 €

FM2-F

FreezeMe Two, Cryopreservation Medium Serum-free

50 ml

70.00 €

TP-A

TumorPlus 263, Complete Medium for Tumor Cells, with L-Glutamine

500 ml

29.90 €

HP-A

HybridomaPrime, Hybridoma Medium, Serum-free

500 ml

34.90 €

HY-A

HybridomaPlus, Hybridoma Medium, Protein-free

500 ml

39.90 €

FP-A

FibroPlus 333 Complete Medium for Fibroblasts, with L-Glutamine

500 ml

29.90 €

ERP-B

EndoPrime Complete Medium for C*tion of Primary Cells

100 ml

on request

ERP-A

EndoPrime Complete Medium for C*tion of Primary Cells

500 ml

on request

HPR-B

HepaPrime Complete Medium for C*tion of Primary Hepatocytes

100 ml

on request

HPR-A

HepaPrime Complete Medium for C*tion of Primary Hepatocytes

500 ml

on request

NKR-G

NK-Prime Complete Medium for C*tion of Primary Natural Killer Cells

20 ml

on request

NKR-B

NK-Prime Complete Medium for C*tion of Primary Natural Killer Cells

100 ml

on request

CHOA-500ML

CHO-ONE A

500 ml

45.00 €

CHOE-500ML

CHO-ONE E

500 ml

49.00 €

CHOE-1000ML

CHO-ONE E

1000ml

89.00 €

CHOF1-100ML

CHO-ONE F1

100 ml

23.00 €

CHOF1-500ML

CHO-ONE F1

500 ml

95.00 €

CHOF2-10ML

CHO-ONE F2

10 ml

15.00 €

CHOF2-50ML

CHO-ONE F2

50 ml

49.00 €

CHO-K1

CHO-ONE Starter Kit

 

169.00 €

CHO-K2

CHO-ONE Expansion Kit

 

950.00 €

DMEM-LPA-P10

DMEM Low Glucose (1 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate

10 L

14.90 €

DMEM-LPA-P50

DMEM Low Glucose (1 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate

50 L

62.90 €

DMEM-HA-P10

DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, w/o Sodium Bicarbonate

10 L

15.50 €

DMEM-HA-P50

DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, w/o Sodium Bicarbonate

50 L

66.50 €

DMEM-HPA-P10

DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate

10 L

15.50 €

DMEM-HPA-P50

DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate

50 L

66.50 €

MEM-A-P10

MEM with Earle’s Salts powder medium, with L-Glutamine, w/o Sodium Bicarbonate

10 L

14.90 €

MEM-A-P50

MEM with Earle’s Salts powder medium, with L-Glutamine, w/o Sodium Bicarbonate

50 L

62.90 €

RPMI-A-P10

RPMI 1640 powder medium, with L-Glutamine, w/o Sodium Bicarbonate

10 L

15.50 €

RPMI-A-P50

RPMI 1640 powder medium, with L-Glutamine, w/o Sodium Bicarbonate

50 L

66.50 €

RPMI-HA-P10

RPMI 1640 powder medium, with L-Glutamine, with 25 mM HEPES, w/o Sodium Bicarbonate

10 L

20.10 €

RPMI-HA-P50

RPMI 1640 powder medium, with L-Glutamine, with 25 mM HEPES, w/o Sodium Bicarbonate

50 L

89.90 €

FBS-52B

Fetal Bovine Serum, Collected in Australia

100 ml

on request

FBS-52A

Fetal Bovine Serum, Collected in Australia

500 ml

on request

FBS-12B

Fetal Bovine Serum, Collected in South America

100 ml

on request

FBS-12A

Fetal Bovine Serum, Collected in South America

500 ml

on request

FBS-22B

Fetal Bovine Serum, Collected in USA

100 ml

on request

FBS-22A

Fetal Bovine Serum, Collected in USA

500 ml

on request

FBS-HI-12B

Fetal Bovine Serum, Heat Inactivated, Collected in South America

100 ml

on request

FBS-HI-12A

Fetal Bovine Serum, Heat Inactivated, Collected in South America

500 ml

on request

FBS-HI-22B

Fetal Bovine Serum, Heat Inactivated, Collected in USA

100 ml

on request

FBS-HI-22A

Fetal Bovine Serum, Heat Inactivated, Collected in USA

500 ml

on request

FBS-11B

Fetal Bovine Serum Advanced, Collected in South America

100 ml

on request

FBS-11A

Fetal Bovine Serum Advanced, Collected in South America

500 ml

on request

FBS-HI-11B

Fetal Bovine Serum Advanced, Heat Inactivated, Collected in South America

100 ml

on request

FBS-HI-11A

Fetal Bovine Serum Advanced, Heat Inactivated, Collected in South America

500 ml

on request

FBS-TET-12B

Fetal Bovine Serum, Tetracycline Negative, Collected in South America

100 ml

on request

FBS-TET-12A

Fetal Bovine Serum, Tetracycline Negative, Collected in South America

500 ml

on request

FBS-ES-22B

Fetal Bovine Serum, ES cell pre-tested, Collected in USA

100 ml

on request

FBS-ES-22A

Fetal Bovine Serum, ES cell pre-tested, Collected in USA

500 ml

on request

FBS-DIA-12B

Fetal Bovine Serum, Dialysed, Collected in South America

100 ml

on request

FBS-DIA-12A

Fetal Bovine Serum, Dialysed, Collected in South America

500 ml

on request

FBS-IG-12B

Fetal Bovine Serum, IgG Stripped, Collected in South America

100 ml

on request

FBS-IG-12B

Fetal Bovine Serum, IgG Stripped, Collected in South America

500 ml

on request

FBS-ED-12F

Fetal Bovine Serum Exosome Depleted

50 ml

on request

FBS-ED-12B

Fetal Bovine Serum Exosome Depleted

100 ml

on request

FBS-ED-12A

Fetal Bovine Serum Exosome Depleted

500 ml

on request

NCS-1B

Newborn Calf Serum

100 ml

12.00 €

NCS-1A

Newborn Calf Serum

500 ml

38.00 €

NCS-HI-1B

Newborn Calf Serum, Heat Inactivated

100 ml

13.10 €

NCS-HI-1A

Newborn Calf Serum, Heat Inactivated

500 ml

43.00 €

CS-1B

Calf Serum

100 ml

8.00 €

CS-1A

Calf Serum

500 ml

29.50 €

ABS-1B

Adult Bovine Serum

100 ml

8.00 €

ABS-1A

Adult Bovine Serum

500 ml

29.50 €

ABS-1C

Adult Bovine Serum

1000 ml

on reques

HOS-1B

Horse Serum

100 ml

7.20 €

HOS-1A

Horse Serum

500 ml

32.00 €

DHS-1B

Donor Horse Serum

100 ml

9.00 €

DHS-1A

Donor Horse Serum

500 ml

35.00 €

GOA-1B

Goat Serum

100 ml

24.00 €

GOA-1A

Goat Serum

500 ml

105.00 €

POR-1B

Porcine Serum

100 ml

10.50 €

POR-1A

Porcine Serum

500 ml

39.00 €

MOU-1B

Mouse Serum

100 ml

499.00 €

RAT-1B

Rat Serum

100 ml

499.00 €

RAB-1B

Rabbit Serum

100 ml

99.00 €

HUM-3B

Human Serum, Type AB, Male

100 ml

134.00 €

BSA-1U

Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA

50 g

56.00 €

BSA-1S

Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA

100 g

77.00 €

BSA-1V

Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA

250 g

on request

BSA-1T

Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA

500 g

299.00 €

BSA-FAF-1U

Bovine Serum Albumin, Fatty Acid Free, Origin USA

50 g

99.00 €

BSA-FAF-1S

Bovine Serum Albumin, Fatty Acid Free, Origin USA

100 g

178.00 €

BSA-PF-1U

Bovine Serum Albumin, Protease Free, Origin USA

50 g

88.00 €

BSA-PF-1S

Bovine Serum Albumin, Protease Free, Origin USA

100 g

151.00 €

HSA-1R

Human Serum Albumin

10 g

56.00 €

HSA-1U

Human Serum Albumin

50 g

227.00 €

HSA-1S

Human Serum Albumin

100 g

420.00 €

N2-K

N2 Supplement (100x), Serum-free

5 ml

45.00 €

C21-H

NCS21 Supplement (50x), Serum-free

10 ml

45.00 €

ITS-H

ITS (100x), Insulin, Transferrin, Selenium

10 ml

25.00 €

HCS-F

Hybridoma Supplément, Serum-free

50 ml

99.00 €

GLN-B

L-Glutamine, 200 mM

100 ml

8.00 €

STA-B

Stable Glutamine, 200 mM

100 ml

14.50 €

NPY-B

Sodium Pyruvate, 100 mM

100 ml

10.50 €

NEAA-B

MEM Nonessential Amino Acids (100x)

100 ml

8.50 €

ACC-1B

Accutase Cell Detachment Solution

100 ml

26.00 €

ACM-1G

AccuMax Cell Detachment Solution

20 ml

29.00 €

ACM-1F

AccuMax Cell Detachment Solution

50 ml

69.00 €

TRY-1B

Trypsin-EDTA (0.05 %) in DPBS (1x)

100 ml

7.00 €

TRY-1B10

Trypsin-EDTA (0.5 %) in DPBS (10x)

100 ml

11.50 €

TRY-2B

Trypsin (0.25 %) in DPBS (1x)

100 ml

7.00 €

TRY-2B10

Trypsin (2.5 %) in DPBS (10x)

100 ml

11.50 €

TRY-3B

Trypsin-EDTA (0.25 %) in HBSS (1x) with Phenol Red

100 ml

9.50 €

TRY-4B

Trypsin-EDTA (0.05 %) in HBSS (1x) with Phenol Red

100 ml

8.50 €

AMP-B

Amphotericin B Solution (250 µg/ml)

100 ml

14.00 €

AAS-B

Antibiotic/Antimycotic Solution (100x)

100 ml

14.00 €

GEN-10B

Gentamicin Solution (10 mg/ml)

100 ml

50.00 €

PS-B

Penicillin/Streptomycin (100x)

100 ml

8.00 €

PSG-B

Penicillin/Streptomycin (100x), with L-Glutamine

100 ml

15.00 €

MYX-B

MycoXpert, Mycoplasma Removal Reagent (50x)

100 ml

99.00 €

LSM-B

Lymphocytes Separation Media, Density 1.077 g/ml

100 ml

12.00 €

LSM-A

Lymphocytes Separation Media, Density 1.077 g/ml

500 ml

36.50 €

FM1-F

FreezeMe One, Cryopreservation Medium with Fetal Bovine Serum

50 ml

65.00 €

FM2-F

FreezeMe Two, Cryopreservation Medium Serum-free

50 ml

70.00 €

MYX-B

MycoXpert, Mycoplasma Removal Reagent (50x)

100 ml

99.00 €

PBS-10XA

Dulbecco's PBS (10x), w/o Ca & Mg, w/o Phenol Red

500 ml

13.00 €

PBS-1A

Dulbecco's PBS (1x), w/o Ca & Mg, w/o Phenol Red

500 ml

6.50 €

PBS-2A

Dulbecco's PBS (1x), with Ca & Mg, w/o Phenol Red

500 ml

8.00 €

HBSS-1A

Hanks' Balanced Salts (1x), with Ca & Mg, w/o Phenol Red

500 ml

7.50 €

HBSS-2A

Hanks' Balanced Salts (1x), w/o Ca & Mg, w/o Phenol Red

500 ml

7.00 €

HBSS-3A

Hanks' Balanced Salts (1x), w/o Ca & Mg, with Phenol Red

500 ml

7.50 €

HEP-B

HEPES Buffer Solution (1 M)

100 ml

39.00 €

PBS-1A-P10

Dulbecco's PBS (1x) powder, w/o Ca & Mg, w/o Phenol Red

10 L

10.90 €

PBS-1A-P50

Dulbecco's PBS (1x) powder, w/o Ca & Mg, w/o Phenol Red

50 L

43.10 €

 

 

 Capricorn Scientific中国代理, Capricorn Scientific上海代理, Capricorn Scientific北京代理,Capricorn Scientific广东代理, Capricorn Scientific江苏代理Capricorn Scientific湖北代理,Capricorn Scientific天津代理, Capricorn Scientific黑龙江代理, Capricorn Scientific内蒙古代理, Capricorn Scientific吉林代理, Capricorn Scientific福建代理,  Capricorn Scientific江苏代理, Capricorn Scientific浙江代理,  Capricorn Scientific四川代理,

 

 

上海金畔生物科技有限公司是实验试剂一站式采购服务商

1:强大的进口辐射能力,血清、抗体、耗材、大部分限制进口品等。

2:产品种类齐全,经营超过700多个品牌,基本涵盖所有生物实验试剂耗材。

3:提供加急服务,货品一般1-2周到货。

4:富有竞争力的价格优势,绝大部分价格有优势。

5:多年积累良好的信誉,大部分客户提供货到付款服务。客户包括清华、北大、交大、复旦、中山等100多所高校,ROCHE,阿斯利康、国药、fisher等药企。

6:我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech;sysy,TriLink BioTechnologies,Inc;worthington-biochem,zyagen等几十家国外公司代理。

7:我们还是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等*批发,欢迎合作。

 

bio-quality CHO残留DNA定量检测试剂盒说明书

科佰迪针对生物制品质量安全控制可提供的产品包括:宿主残留DNA样本前处理试剂盒和各种定量检测试剂盒(如:人源、HEK293、CHO、E.coli、Vero、NS0以及Pichia等工程细胞以及猪源性残留DNA检测试剂盒等),同时也可根据客户需求进行定制化开发。该类试剂盒具备快速、高效、准确及性价比高的特点。科佰迪也提供通用类的生物检测试剂:如:2×BQStar SBYR Green qPCR Master Mix(with or without UNG)、2×BQStar TaqMan qPCR Master Mix(with or without UNG)、2×BQStar Eva Green qPCR Master Mix(with or without UNG)、2×ColorVisual LAMP Master Mix、PCR产物纯化试剂盒、病毒DNA/RNA提取试剂盒(磁珠法)等。


bio-quality CHO残留DNA定量检测试剂盒说明书

CHO残留DNA定量检测试剂盒
产品介绍

CHO残留DNA定量检测试剂盒用于定量检测各种生物制品产品的中间品、半成品和成品中CHO宿主细胞残留DNA的残留量,定量检测试剂盒中配有CHO DNA定量参考品。CHO残留DNA定量检测试剂盒具有方便快捷、性能可靠、灵敏度高、平台适应性广的特点。

产品特点:

检测样本类型 各种复杂类型生物制品的中间品、半成品、灌装原液等
检测原理 TaqMan探针法
平台兼容性广 可兼容不同的检测平台
灵敏度高 检测限可低至fg级
方便快捷 上机检测时间仅1.5h

Canopy CHO HCP 检测试剂盒 CHO宿主细胞蛋白ELISA检测试剂盒 货号:HCP001

Canopy CHO HCP 检测试剂盒 CHO宿主细胞蛋白ELISA检测试剂盒 货号:HCP001

中国仓鼠卵巢宿主细胞蛋白ELISA试剂盒旨在定量测量CHO表达系统中宿主 细胞蛋白残留量。

提供的浓缩CHO蛋白用于生产测定的标准曲线。将CHO标准品或未知样品加入提供的96孔板中,96孔板已预先包被了抗CHP HCP抗体,以从生物样品中捕获CHO蛋白,然后添加与生物素偶联的二抗抗CHO HCP抗体,并进行孵育以使其与捕获的CHO蛋白结合,再添加链霉亲和素-HRP结合物,使其结合到被生物素标记的抗体上,经洗涤后加入TMB底物显色。

HCP的量与产物的颜色成正比。最后加入终止液终止反应,并在450nm波长的酶标仪上测定吸光度(OD值)。根据CHO蛋白标准品生成的 标准曲线,并考虑未知样品的稀释度,计算未知样品中CHO蛋白的浓度。为保证样品的浓度落在标准曲线的线性范围内,可以通过预实验来确定样品 的最佳稀释度。

注意: 叠氮化钠会干扰检测,不应用于样品或缓冲液中。


试剂盒组分

包被的96孔板、CHO 蛋白标准品、5x 稀释缓冲液、10x PBS-T、报告抗体、链霉亲和素-HRP 结合物、TMB 底物、终止液、封板膜


产品特点

孔间差异小

批次间具有一致性


产品优势

覆盖范围

Canopy CHO 宿主细胞蛋白抗体与竞品C 三代抗体使用相同的检测方法即2D免疫印迹分析,比较结果表明Canopy抗体的覆盖范围比竞争对手提高25%。


HCP检测

Canopy CHO HCP 检测试剂盒和竞品C三代检测试剂盒检测三种不同的DSs (由CHO产生的治疗性抗体)中宿主细胞蛋白的含量,结果显示 Canopy 的抗体检测到的宿主细胞蛋白含量是竞品的2-8倍。


孔间精准度验证

Canopy CHO HCP 抗体的孔间变异比竞品C小。


更多产品信息,请直接与我们联系!



moleculardevices抗CHO HCP检测试剂盒


Molecular Devices 始创于上世纪80年代美国硅谷,作为 高通量仪器设备的品牌,一直致力于为生命科学研 究及药物研发提供先进的解决方案。其产品覆盖 微孔板检测分析、高通量筛选、高内涵成像、克隆筛 选等。公司以持续创新、快速、先进质量的产品及完 善的售后服务为宗旨服务客户。

 

moleculardevices抗CHO HCP检测试剂盒

ANTI-CHO HCP DETECTION KIT – PACK

  • $4,256.00

货号:18-5123

宿主细胞蛋白质检测试剂盒,用于定量生物处理样品中残留的CHO宿主细胞蛋白质。包括5个分析试剂盒,每个试剂盒包含一个托盘,其中96个生物传感器涂有Cygnus 3g抗CHO HCP抗体,以及分析所有当前八位体系统上96个HCP样品所需的所有校准品、试剂和缓冲液。含10%的托盘价格折扣。

 

 

德国biogenes CHO宿主蛋白检测试剂盒


德国biogenes CHO宿主蛋白检测试剂盒

CHO|360-HCP ELISA

CHO | 360-HCP ELISA是增强的通用宿主细胞蛋白(HCP)测定。它被设计为覆盖更广泛的中国仓鼠卵巢(CHO)宿主细胞蛋白(HCP),而不是其他商业上可获得的通用HCP测定。

CHO | 360-HCP ELISA基于*的分析设计,采用不同制备的HCP抗原和来自两个物种的抗体:

  • 抗原由从CHO-K1和CHO-S的模拟发酵获得的总HCP和分级HCP制备 
  • 通过免疫山羊和兔子产生特异性抗体
  • 方法得到四种不同的HCP ELISA试剂盒(A型至D型),共同构建增强型通用CHO | 360-HCP试验
     
类型 种类 HCP
A型 山羊 总HCP
B型 山羊 分级HCP
C型 兔子 总HCP
D型 兔子 分级HCP

CHO | 360-HCP ELISA试剂盒(A至D)的灵敏度

  • LOD介于0.5 – 1,0 ng / mL之间
  • LOQ在2 – 3 ng / mL之间
  • 工作范围2 – 100 ng / mL

图1:对于不同的CHO HCP标准浓度,通过测量的OD值(A450)的非线性回归获得所有四种CHO | 360-HCP ELISA(A型至D型)的标准曲线。

HCP恢复很大程度上取决于所用ELISA试剂盒的类型

“没有一种通用的HCP ELISA适用于所有样品,建议用户在做出选择前测试不同的试剂盒。”

为了举例说明,我们在大量模拟CHO HCP样品的基础上测试了CHO | 360-HCP ELISA试剂盒(A至D)和市售的通用CHO HCP测定法。图2显示,在大多数情况下,使用四种CHO | 360-HCP ELISAs试剂盒中的一种估算出宜回收率。在样品4的情况下,用ELISA试剂盒D型估计回收率> 90%,而ELISA试剂盒A,B和C以及商业试剂盒不太敏感,后者仅识别20%的宿主细胞蛋白质。

图2:使用五种不同的CHO HCP测定 – CHO | 360-HCP ELISA试剂盒类型(A至D)和市售的通用CHO HCP测定法测试选择的模拟CHO HCP样品。高于100%的回收率是基于高估。

产品概述

产品

产品代码

CHO | 360-HCP ELISA入门套装

IP 001

CHO | 360-HCP ELISA试剂盒A型

IP 101

CHO | 360-HCP ELISA试剂盒B型

IP 102

CHO | 360-HCP ELISA试剂盒C型

IP 103

CHO | 360-HCP ELISA试剂盒D型

IP 104

抗CHO抗体A型

IP 111

抗CHO抗体B型

IP 112

抗CHO抗体C型

IP 113

抗CHO抗体D型

IP 114

CHO | 360-HCP标准

IP 120

CHO | 360-HCP分析缓冲液

IP 130 

 

品名 货号 欧元
CHO|360-HCP ELISA Starter Set IP 001 1605
CHO|360-HCP ELISA Kit Type A IP 101 535
CHO|360-HCP ELISA Kit Type B IP 102 535
CHO|360-HCP ELISA Kit Type C IP 103 535
CHO|360-HCP ELISA Kit Type D IP 104 535
Anti-CHO antibody Type A IP 111 485
Anti-CHO antibody Type B IP 112 485
Anti-CHO antibody Type C IP 113 485
Anti-CHO antibody Type D IP 114 485
CHO|360-HCP Standard IP 120 1300
CHO|360-HCP Assay Buffer IP 130 150
E.coli|360 HCP ELISA Type A-D Starter Set IP 2001 1605
E.coli|360 HCP ELISA BL21(DE3) Starter Set IP 2002 805
E.coli|360-HCP ELISA W3110 Starter Set IP 2003 805
E.coli|360-HCP ELISA Type A IP 201 535
E.coli|360-HCP ELISA Type B IP 202 535
E.coli|360-HCP ELISA Type C IP 203 535
E.coli|360-HCP ELISA Type D IP 204 535
Anti-E.coli antibody Type A IP 211 485
Anti-E.coli Antibody Type B IP 212 485
Anti-E.coli antibody Type C IP 213 485
Anti-E.coli antibody Type D IP 214 485
E.coli|360-HCP Standard Type A IP 221 1300
E.coli|360-HCP Standard Type B IP 222 1300
E.coli|360-HCP Standard Type C IP 223 1300
E.coli|360-HCP Standard Type D IP 224 1300
E.coli|360-HCP Assay Buffer IP 230 150
BlueCap LX-Buffer S 201 52
BlueCap LX-Buffer S 201 116
BlueCap LX-Buffer S 201 439
BlueCap Blocking Solution S 204 52
BlueCap Blocking Solution S 204 116
BlueCap Blocking Solution S 204 439
BlueCap Antibody Stabilizer TRIS-based S 208 48
BlueCap Antibody Stabilizer TRIS-based S 208 105
BlueCap Antibody Stabilizer TRIS-based S 208 315
BlueCap Washing Buffer Tris based no Tween 500ml S 211.500 36
BlueCap POD Stabilizer S 220 31
BlueCap POD Stabilizer S 220 63
BlueCap POD Stabilizer S 220 149