PhyNexus Streptavidin Columns 说明书

 PhyNexus在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品,的服务,为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

PhyNexus中国代理PhyNexus上海代理, PhyNexus北京代理,PhyNexus广东代理, PhyNexus江苏代理PhyNexus湖北代理,PhyNexus天津,PhyNexus黑龙江代理,PhyNexus内蒙古代理,PhyNexus吉林代理,PhyNexus福建代理, PhyNexus江苏代理, PhyNexus浙江代理, PhyNexus四川代理,

Douglas Gjerde于2002年在加利福尼亚州的圣何塞市建立了PhyNexus公司,以调查蛋白质样品制备的需求。专栏和机器人被开发小型化和自动化过程,当保留蛋白质的自然状态时。2005年,引进了一整列的专栏和机器人。在接下来的几年中,柱子被修改为与所有主要的机器人液体处理器兼容。定期提供超过20种色谱柱的化学品,提供超过100种定制包装的产品。从2016年开始,引入了试剂盒和仪器,大大增加了瞬时转染质粒的可用性。PhyNexus客户是制药公司和学术界的生命科学家。

 

链霉亲和素列

现代Target Discovery的挑战需要复杂检测的自动化。免疫沉淀(IP)分析已被证明在研究发现中非常有用,以鉴定分子复合物的组分,发现蛋白质 – 蛋白质和蛋白质 – 核酸的相互作用,并量化免疫原性应答。

蛋白APhyTip®色谱柱已成功用于此目的,但是只有当目标肽或蛋白质具有抗体时,IP才能成功。生物素标记试剂的可用性允许研究人员通过将感兴趣的蛋白质缀合到抗生蛋白链菌素树脂上来生成定制的亲和树脂。这些定制树脂随即准备从生物样品中取出相互作用的蛋白质和复合物。为了有效地自动化这些分析,必须处理重要的样品制备步骤。也就是说,蛋白质需要被纯化,必须去除过量的生物素标记试剂,生物素化的蛋白质需要被固定。

PhyNexus亲和纯化,含5K分子量截留树脂的凝胶过滤柱和链霉亲和素柱联合使用,是小批量,自动化,高通量纯化,分离和固定生物素化蛋白用于复杂测定的理想工具。

 

 

 

 

 

 

 

 

PhyTip色谱柱是PhyNexus*的捕获,纯化和富集工具,专为实验室规模的蛋白质样品制备而设计。PhyTip色谱柱适用于各种液体处理平台,并含有特定的亲和树脂以满足特定的应用要求。

PhyNexus建议使用生物素标记的牛血清白蛋白(Sigma,A8549)作为标准来验证PhyTip色谱柱在所有应用中的使用。

纯化和富集的样品必须清晰,无颗粒物质。强烈建议离心样品,并使用PhyTip色谱柱前仅使用清澈的上清液。

 

链霉抗生物素蛋白PhyTip柱和链霉亲和素的PhyTip色谱柱已经过优化,可用于一般分子生物学实验室试剂和特定的PhyNexus仪器流速/体积,如下所示。这些信息是使用PhyNexus纯化系统收集的。

PhyNexus建议使用以下缓冲区(不提供):

捕获缓冲液 – pH值为7.4的磷酸盐缓冲溶液,对于需要添加额外缓冲液以补充样品体积并确保捕获的正确pH值的情况

清洗缓冲液I – 磷酸盐缓冲液pH 7.4

清洗缓冲液II – 磷酸盐缓冲液pH 7.4

富集缓冲液 – 如果进行下拉测定,zui终的洗脱步骤可能与酸性缓冲液如磷酸盐缓冲液(pH 2.5)

中和缓冲液。 – Tris缓冲液pH9.0

 

PhyNexus建议使用含有IgG的PhyTip入门试剂盒作为标准来验证PhyTip色谱柱在所有应用中的使用。

纯化和富集的样品必须清晰,无颗粒物质。强烈建议离心样品,并使用PhyTip色谱柱前仅使用清澈的上清液。

运输和存储

每包PhyTip色谱柱已经制造并按照zui高标准进行了质量控制,并运送到保持盒中,以保持每个PhyTip色谱柱内特定亲和树脂的完整性。本产品在环境温度下运输,但收到时应存放在标准的实验室冰箱中,温度在4℃到8℃之间。

  • 不要冻结或冻结。
  • 不使用时,请将箱子的盖子关好并密封好,存放在冰箱内。
  • 不要让亲和树脂在干燥的环境中长时间存放干燥。

链霉亲和素的PhyTip柱在从PhyNexus运输时储存在甘油中。

 

部分产品信息如下:

Streptavidin Agilent Velocity 11 Bravo & V-Prep 200 96 20 PTV 92-20-05 Box of 96 PhyTip columns (200 µL volume) 20 µL of Streptavidin per column
Streptavidin Beckman FX & NX 96-well 200 96 5 PTB 92-05-05 Box of 96 PhyTip columns (200 µL volume) 5 µL of Streptavidin per column
Streptavidin Beckman FX & NX 96-well 200 96 20 PTB 92-20-05 Box of 96 PhyTip columns (200 µL volume) 20 µL of Streptavidin per column
Streptavidin Caliper SciClone & Zephyr 96 channel_now Perkin Elmer 200 96 5 PTZ 92-05-05 Box of 96 PhyTip columns (200 µL volume) 5 µL of Streptavidin per column
Streptavidin Caliper SciClone & Zephyr 96 channel_now Perkin Elmer 200 96 20 PTZ 92-20-05 Box of 96 PhyTip columns (200 µL volume) 20 µL of Streptavidin per column
Streptavidin Dynamic Devices Oasis & Lynx 1000 96 10 PTD 91-10-05 Box of 96 PhyTip columns (1 mL volume) 10 µL of Streptavidin affinity resin per column
Streptavidin Dynamic Devices Oasis & Lynx 1000 96 20 PTD 91-20-05 Box of 96 PhyTip columns (1 mL volume) 20 µL of Streptavidin affinity resin per column
Streptavidin Dynamic Devices Oasis & Lynx 1000 96 40 PTD 91-40-05 Box of 96 PhyTip columns (1 mL volume) 40 µL of Streptavidin affinity resin per column
Streptavidin Dynamic Devices Oasis & Lynx 1000 96 80 PTD 91-80-05 Box of 96 PhyTip columns (1 mL volume) 80 µL of Streptavidin affinity resin per column
Streptavidin Dynamic Devices Oasis & Lynx 1000 96 160 PTD 91-16-05 Box of 96 PhyTip columns (1 mL volume) 160 µL of Streptavidin affinity resin per column

 

我们公司zui大优势是强大的采购,

1:基本什么都能进口,血清,抗体,耗材,还有部分限制进口的,

2:货品全,现经营过700多个品牌,基本所有生物试剂耗材都可以进口,特别是冷偏的产品那就更有优势,

3:提供加急服务,一般1-2周到货,超过时限加急费全免

4:价格公道,绝大部分价格有优势,当然不能保证100%产品都是,因为意味着没有服务.

5:良好的信誉,大部分客户我们提供货到付款服务,客户包括清华,北大 交大 复旦,中山等100多所大学,ROCHE,阿斯利康,国药 ,fisher等500多家公司

6:我们*代理的品牌有:Antibody Research Corporation,arcticzymes,Biorelevant,AmberGen, Inc. ,clemente-associates,clodronaiposomes,Columbia Biosciences,enzyme research,Gene Bridges GmbH,Genovis,AmberGen, Inc.  Biotechnology GmbH,Haematologic Technologies HTI Haemtech,hookelabs,Immudex,Innovative Research of America,inspiralis ,List Biological Laboratories, Inc.,lumafluor,Microsurfaces,multiplicom,nanotools,Pel-Freez Biologicals,pentapharm,progen,Protein Ark,QA-Bio,Inc,QA-Bio,IncQuickZyme Biosciences,Teknova,TriLink BioTechnologies, Inc.,Zyagen Laboratories 等

7:我们还是invitrogen,qiagen,Midland BioProducts Corporationam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知名*批发,欢迎合作。

 

加拿大Silicycle硅胶填料SiliaChrom HPLC Columns, dt C18, 10 µm, 100 Å (H141807E)

上海金畔生物科技有限公司代理加拿大Silicycle硅胶填料色谱柱层析板金属离子清除剂全线产品,欢迎访问官网了解更多产品信息和订购。
SiliaChrom HPLC Columns, dt C18, 10 µm, 100 Å (H141807E)

SiliaChrom HPLC Columns, dt C18, 10 µm, 100 Å (H141807E)

Prices do not include applicable taxes, customs, duties and/or shipping.

Product Number Quantity Length Internal Diameter  
H141807E-N250 1/box 250 mm 4.6 mm

具体价格咨询客服

SiliaChrom HPLC Columns, dt C18, 10 µm, 100 Å (H141807E)   Loading…  

Details

SiliaChrom dt C18 is a universal column compatible with 100 % aqueous conditions. It shows good peak shapes for any type of molecules (acidic, neutral, basic). The ultra pure metal-free silica allows high sensitivity for LC/MS applications. SiliaChrom dt C18 columns are available in 3, 5 & 10 µm.

SiliaChrom dt C18 Main Characteristics:

  • Compatible with 100 % aqueous and 100 % organic mobile phases
  • Ultra pure metal-free silica (99.999 % purity)
  • High sensitivity for LC/MS applications
  • Enhanced retention of hydrophilic compounds
  • Inertness for acidic and basic analytes
  • Endcapped

Additional Information

Particle Shape Spherical
Array dt C18
Family / Formats SiliaChrom® (HPLC Columns)
Particle Size 10 µm
Pore Size 100 Å
Chromatographic Mode Reversed Phases
USP Code L1

加拿大Silicycle硅胶填料SiliaChrom HPLC Columns, dt C18, 5 µm, 100 Å (H141805E)

上海金畔生物科技有限公司代理加拿大Silicycle硅胶填料色谱柱层析板金属离子清除剂全线产品,欢迎访问官网了解更多产品信息和订购。
SiliaChrom HPLC Columns, dt C18, 5 µm, 100 Å (H141805E)

SiliaChrom HPLC Columns, dt C18, 5 µm, 100 Å (H141805E)

Prices do not include applicable taxes, customs, duties and/or shipping.

Product Number Quantity Length Internal Diameter  
H141805E-N050 1/box 50 mm 4.6 mm

具体价格咨询客服

H141805E-N100 1/box 100 mm 4.6 mm

具体价格咨询客服

H141805E-N150 1/box 150 mm 4.6 mm

具体价格咨询客服

H141805E-N250 1/box 250 mm 4.6 mm

具体价格咨询客服

SiliaChrom HPLC Columns, dt C18, 5 µm, 100 Å (H141805E)   Loading…  

Details

SiliaChrom dt C18 is a universal column compatible with 100 % aqueous conditions. It shows good peak shapes for any type of molecules (acidic, neutral, basic). The ultra pure metal-free silica allows high sensitivity for LC/MS applications. SiliaChrom dt C18 columns are available in 3, 5 & 10 µm.

SiliaChrom dt C18 Main Characteristics:

  • Compatible with 100 % aqueous and 100 % organic mobile phases
  • Ultra pure metal-free silica (99.999 % purity)
  • High sensitivity for LC/MS applications
  • Enhanced retention of hydrophilic compounds
  • Inertness for acidic and basic analytes
  • Endcapped

Additional Information

Particle Shape Spherical
Array dt C18
Family / Formats SiliaChrom® (HPLC Columns)
Particle Size 5 µm
Pore Size 100 Å
Chromatographic Mode Reversed Phases
USP Code L1

加拿大Silicycle硅胶填料SiliaChrom Plus HPLC Columns, Amine (WAX), 10 µm, 100 Å (HPL-S52007E-A)

上海金畔生物科技有限公司代理加拿大Silicycle硅胶填料色谱柱层析板金属离子清除剂全线产品,欢迎访问官网了解更多产品信息和订购。
SiliaChrom Plus HPLC Columns, Amine (WAX), 10 µm, 100 Å (HPL-S52007E-A)

SiliaChrom Plus HPLC Columns, Amine (WAX), 10 µm, 100 Å (HPL-S52007E-A)

Prices do not include applicable taxes, customs, duties and/or shipping.

Product Number Quantity Length Internal Diameter  
HPL-S52007E-A-N150 1/box 150 mm 4.6 mm

具体价格咨询客服

HPL-S52007E-A-N250 1/box 250 mm 4.6 mm

具体价格咨询客服

HPL-S52007E-A-Q150 1/box 150 mm 10 mm

具体价格咨询客服

HPL-S52007E-A-Q250 1/box 250 mm 10 mm

具体价格咨询客服

HPL-S52007E-A-T050 1/box 50 mm 21.2 mm

具体价格咨询客服

HPL-S52007E-A-T100 1/box 100 mm 21.2 mm

具体价格咨询客服

HPL-S52007E-A-T150 1/box 150 mm 21.2 mm

具体价格咨询客服

HPL-S52007E-A-T250 1/box 250 mm 21.2 mm

具体价格咨询客服

HPL-S52007E-A-V050 1/box 50 mm 30 mm

具体价格咨询客服

HPL-S52007E-A-V100 1/box 100 mm 30 mm

具体价格咨询客服

HPL-S52007E-A-V150 1/box 150 mm 30 mm

具体价格咨询客服

HPL-S52007E-A-V250 1/box 250 mm 30 mm

具体价格咨询客服

HPL-S52007E-A-W050 1/box 50 mm 50 mm

具体价格咨询客服

HPL-S52007E-A-W100 1/box 100 mm 50 mm

具体价格咨询客服

HPL-S52007E-A-W150 1/box 150 mm 50 mm

具体价格咨询客服

HPL-S52007E-A-W250 1/box 250 mm 50 mm

具体价格咨询客服

SiliaChrom Plus HPLC Columns, Amine (WAX), 10 µm, 100 Å (HPL-S52007E-A)   Loading…  

Details

SiliaChrom Plus Amine columns are very popular for sugars analysis (carbohydrates, glycosides and oligosaccharides). They represent a good alternative for normal phase separation of compounds with acidic/basic properties. SiliaChrom Plus Amine columns can also be used in Ion Exchange Mode, for retention & release of very strong anions such as sulfonic acids.

We guarantee:

  • Ultra pure metal-free silica (99.9999 % purity)
  • High column performance and resolution
  • Enhanced batch-to-batch reproducibility
  • Extended column lifetime
  • Reduced silanol activity, better peak symmetry
  • Extremely low bleeding for LC/MS applications
  • Easy scale-up to preparative formats

Additional Information

Particle Shape Irregular
Array Amine (WAX)
Family / Formats SiliaChrom® (HPLC Columns)
Particle Size 10 µm
Pore Size 100 Å
Chromatographic Mode Normal Phases
USP Code L8

加拿大Silicycle硅胶填料SiliaChrom Plus HPLC Columns, Amine (WAX), 5 µm, 100 Å (HPL-S52005E-A)

上海金畔生物科技有限公司代理加拿大Silicycle硅胶填料色谱柱层析板金属离子清除剂全线产品,欢迎访问官网了解更多产品信息和订购。
SiliaChrom Plus HPLC Columns, Amine (WAX), 5 µm, 100 Å (HPL-S52005E-A)

SiliaChrom Plus HPLC Columns, Amine (WAX), 5 µm, 100 Å (HPL-S52005E-A)

Prices do not include applicable taxes, customs, duties and/or shipping.

Product Number Quantity Length Internal Diameter  
HPL-S52005E-A-G033 1/box 33 mm 2.1 mm

具体价格咨询客服

HPL-S52005E-A-G050 1/box 50 mm 2.1 mm

具体价格咨询客服

HPL-S52005E-A-G100 1/box 100 mm 2.1 mm

具体价格咨询客服

HPL-S52005E-A-G150 1/box 150 mm 2.1 mm

具体价格咨询客服

HPL-S52005E-A-G250 1/box 250 mm 2.1 mm

具体价格咨询客服

HPL-S52005E-A-H033 1/box 33 mm 3.0 mm

具体价格咨询客服

HPL-S52005E-A-H050 1/box 50 mm 3.0 mm

具体价格咨询客服

HPL-S52005E-A-H100 1/box 100 mm 3.0 mm

具体价格咨询客服

HPL-S52005E-A-H150 1/box 150 mm 3.0 mm

具体价格咨询客服

HPL-S52005E-A-H250 1/box 250 mm 3.0 mm

具体价格咨询客服

HPL-S52005E-A-N033 1/box 33 mm 4.6 mm

具体价格咨询客服

HPL-S52005E-A-N050 1/box 50 mm 4.6 mm

具体价格咨询客服

HPL-S52005E-A-N100 1/box 100 mm 4.6 mm

具体价格咨询客服

HPL-S52005E-A-N150 1/box 150 mm 4.6 mm

具体价格咨询客服

HPL-S52005E-A-N250 1/box 250 mm 4.6 mm

具体价格咨询客服

HPL-S52005E-A-Q150 1/box 150 mm 10 mm

具体价格咨询客服

HPL-S52005E-A-Q250 1/box 250 mm 10 mm

具体价格咨询客服

HPL-S52005E-A-T050 1/box 50 mm 21.2 mm

具体价格咨询客服

HPL-S52005E-A-T100 1/box 100 mm 21.2 mm

具体价格咨询客服

HPL-S52005E-A-T150 1/box 150 mm 21.2 mm

具体价格咨询客服

HPL-S52005E-A-T250 1/box 250 mm 21.2 mm

具体价格咨询客服

HPL-S52005E-A-V050 1/box 50 mm 30 mm

具体价格咨询客服

HPL-S52005E-A-V100 1/box 100 mm 30 mm

具体价格咨询客服

HPL-S52005E-A-V150 1/box 150 mm 30 mm

具体价格咨询客服

HPL-S52005E-A-V250 1/box 250 mm 30 mm

具体价格咨询客服

SiliaChrom Plus HPLC Columns, Amine (WAX), 5 µm, 100 Å (HPL-S52005E-A)   Loading…  

Details

SiliaChrom Plus Amine columns are very popular for sugars analysis (carbohydrates, glycosides and oligosaccharides). They represent a good alternative for normal phase separation of compounds with acidic/basic properties. SiliaChrom Plus Amine columns can also be used in Ion Exchange Mode, for retention & release of very strong anions such as sulfonic acids.

We guarantee:

  • Ultra pure metal-free silica (99.9999 % purity)
  • High column performance and resolution
  • Enhanced batch-to-batch reproducibility
  • Extended column lifetime
  • Reduced silanol activity, better peak symmetry
  • Extremely low bleeding for LC/MS applications
  • Easy scale-up to preparative formats

Additional Information

Particle Shape Irregular
Array Amine (WAX)
Family / Formats SiliaChrom® (HPLC Columns)
Particle Size 5 µm
Pore Size 100 Å
Chromatographic Mode Normal Phases
USP Code L8