cytoskeleton APHL95说明书


 

cytoskeleton APHL95说明书

肌动蛋白产品

 

 

肌动蛋白是一种43 kDa的蛋白质,在物种之间非常高度保守。肌动蛋白具有三种主要同种型(α-肌动蛋白,β-肌动蛋白和γ-肌动蛋白),其在同种型之间显示> 90%的氨基酸(aa)同源性,并且在特定同种型组的成员中具有> 98%的同源性。大多数同种型异质性位于氨基末端30个残基中。肌动蛋白的氨基末端位于F-肌动蛋白(4)中双螺旋的外围,并且该位点也被认为与肌球蛋白(5)相互作用。

 

Cytoskeleton,Inc。提供大量高纯度和生物活性肌动蛋白,肌动蛋白结合蛋白,抗体,缓冲液和研究Biochem Kits™。我们的肌动蛋白生物化学试剂盒使研究人员可以轻松获得该领域中强大的体内体外试验。

 

蛋白质

在全面的肌动蛋白组合中提供的活动和纯度。 

 

  • 选择> 95%或> 99%纯
  • 标记或未标记的蛋白质
  • 细胞质,心脏,骨骼和平滑肌来源
  • 批量可用

 

肌动蛋白的纯度和生物活性。  在SDS-PAGE凝胶上运行
100μg> 99%纯度(AKL99,AD99,APHL99,AS99)和> 95%纯(AKL95)兔骨骼肌肌动蛋白,并用考马斯蓝染色。在聚合试验中,箭头表示肌动蛋白,箭头表示α-辅肌动蛋白污染物(115kDa)。纯化的肌动蛋白中的微量杂质主要是肌动蛋白结合蛋白,例如α-辅肌动蛋白和凝溶胶蛋白。AKL99产生> 90%的F-肌动蛋白,AKL95产生> 80%的F-肌动蛋白。

 

 

肌动蛋白(纯度> 95%):人血小板 – 停产 APHL95
 
肌动蛋白(纯度> 95%):兔骨骼肌 AKL95
 
肌动蛋白(纯度> 99%):牛心肌 AD99
 
肌动蛋白(纯度> 99%):鸡g肌肉 AS99
 
肌动蛋白(纯度> 99%):人血小板 APHL99
 
肌动蛋白(纯度> 99%):兔骨骼肌 AKL99
 
肌动蛋白(生物素):骨骼肌 AB07
 
肌动蛋白(预成形细丝):兔骨骼肌 AKF99
 
肌动蛋白(芘标记):兔骨骼肌 AP05
 
肌动蛋白(罗丹明):人血小板 APHR
 
肌动蛋白(罗丹明):兔骨骼肌 AR05
 
心脏薄丝复合物(CTFC) CS-TFC01

 

 

 

 

Cytoskeleton BK163-S说明书

Cytoskeleton,Inc。成立于1993年。从一开始我们就不断扩展我们的产品线。

Cytoskeleton,Inc。很高兴为药物筛选,信号转导和细胞骨架研究提供广泛的试剂盒和产品。我们专注于生产纯化蛋白质和易于使用的试剂盒,以研究生物化学和细胞过程。我们的试剂盒可用于少量样品,用于基础研究或小屏幕(Biochem Kits™),也可用于大屏幕的高通量样品(CytoDYNAMIX Screens™)。

除了我们现有的产品,我们还在产品线中为微管,微管蛋白,运动蛋白,小G蛋白效应物,GAP,GEF和其他几种蛋白提供药物筛选服务。

Cytoskeleton BK163-S说明书

 

Signal-Seeker™乙酰赖氨酸检测试剂盒(10次检测)

10种检测方法(Cat。#BK163-S)

Signal-Seeker™系列产品的开发旨在简化专家和非专业人士对关键调节蛋白修饰的分析。全面的Signal-Seeker™试剂盒提供亲和珠系统,可从任何给定的细胞或组织裂解液中分离和富集修饰的蛋白质。然后使用针对靶蛋白的抗体通过标准蛋白质印迹程序分析富集的蛋白质群体。

产品用途包括

  • 调查瞬态监管机制
  • 测量多种途径成员蛋白的信号传导事件 
  • 发现您感兴趣的蛋白质的新修改
  • 深入了解监管机制
  • 测量内源性或瞬时表达的蛋白质信号传导事件

 

套件内容

乙酰赖氨酸试剂盒包含以下组分:

裂解和蛋白质定量步骤 IP和预清除步骤 洗步骤 洗脱步骤 西部一步

 BlastR™裂解缓冲液

 BlastR™稀释缓冲液

 BlastR™过滤器

 蛋白酶抑制剂鸡尾酒

 HDAC抑制剂鸡尾酒

 Precision Red™蛋白质分析试剂

 乙酰赖氨酸亲和珠

 IP控制珠

 

 

 

 

 BlastR™洗涤缓冲液

 

 

 

 

 

 旋转列

 珠子洗脱缓冲液

 

 

 

 

 化学发光试剂A.

 化学发光试剂B.

 抗乙酰赖氨酸-HRP抗体

 

 

 

 

示例结果

这些套件有很多应用,这里我们描述一个有趣的例子:

应用1:调查显着的乙酰化事件

使用Signal-Seeker™乙酰赖氨酸检测试剂盒进行免疫沉淀,与细胞信号传导乙酰赖氨酸抗体和免疫组化乙酰赖氨酸亲和珠相比较

图1.各种乙酰基 – 赖氨酸亲和试剂用于来自Cos-7细胞的IP乙酰化蛋白质,用TSA(1m M)和烟酰胺(1mM)处理(+)或未处理( – )6小时。(1)16.7  AAC04珠浆液(20升克抗体)。(2)50  AAC04珠浆液(60升克抗体)。(3)抗乙酰赖氨酸兔单克隆混合物(Cell Signaling,按制造商的说明书1:100)。(4)乙酰ImmuneChem赖氨酸亲和力珠(40 克抗体)。(5)ImmunChem乙酰赖氨酸珠(80 克抗体)。(6)正常小鼠IgG对照珠(60 g抗体)。洗脱富集的乙酰化蛋白质的总曲线,并通过蛋白质印迹用AAC03-HRP抗体(1:3000)分析。AAC04在富集广泛的乙酰化蛋白质方面表现出色,而其他商业乙酰赖氨酸富集试剂主要富集丰富的乙酰化蛋白质(例如乙酰化微管蛋白和组蛋白)。

使用Signal-Seeker™乙酰赖氨酸检测试剂盒免疫沉淀靶特异性乙酰化蛋白

图2A:使用BlastR缓冲液分离A431细胞,未处理( – )或用1m M TSA和1mM烟酰胺处理(+)6小时。使用AAC04珠(60m g)进行IP 。通过SDS-PAGE分离总细胞裂解物(输入)和免疫沉淀的样品,并通过蛋白质印迹用针对EGFR的抗体(Millipore,1:1000),Hsp90(Abcam,1:20,000),P53(Sigma,1:2000)进行分析,和RhoDGI(Millipore,1:1000)

使用Signal-Seeker™乙酰赖氨酸检测试剂盒从肝脏和心脏组织中免疫沉淀乙酰化蛋白

图2B:用BlastR缓冲液获得小鼠组织提取物(肝脏和心脏)。使用AAC04珠(60,进行IP  g)或mIgG对照珠(#CIG02,60 在组织提取物的1mg的克)。通过SDS-PAGE分离富集的蛋白质,并通过蛋白质印迹用AAC03-HRP(1:3000)分析。

Cytoskeleton品牌代理商

上海金畔生物科技有限公司是Cytoskeleton品牌代理商 ,欢迎访问官网了解更多产品信息和订购。
Cytoskeleton

简要描述:

细胞骨架公司很高兴提供范围广泛的工具包和产品用于药物筛选、信号转导和细胞骨架研究。

细胞骨架公司很高兴提供范围广泛的工具包和产品用于药物筛选、信号转导和细胞骨架研究。


Cytoskeleton, Inc. was formed in 1993. Since the start we have continuously been expanding our line of products.
Cytoskeleton, Inc. is pleased to offer a wide range of kits and products for drug screening, signal transduction and cytoskeletal research. We specialize in the production of purified proteins and easy-to-use kits to study biochemical and cellular processes. Our kits are either made for smaller number of samples for use in basic research or small screens (Biochem Kits™) or in a high throughput scale for large screens (CytoDYNAMIX Screens™).


品牌

货号

名称

规格

Cytoskeleton

BK165-S

NEW Signal-Seeker™ SUMOylation 1 Detection Kit (10 assay)

10tests

Cytoskeleton

BK 165

NEW Signal-Seeker™ SUMOylation 1 Detection Kit (30 assay)

30tests

Cytoskeleton

BK 163-S

Signal-Seeker™ Acetyl-Lysine Detection Kit (10 assay)

10tests

Cytoskeleton2019年价格表

 

 

Cytoskeleton,Inc。很高兴为药物筛选,信号转导和细胞骨架研究提供广泛的试剂盒和产品。我们专注于生产纯化蛋白质和易于使用的试剂盒,以研究生物化学和细胞过程。我们的试剂盒可用于少量样品,用于基础研究或小屏幕(Biochem Kits™),也可用于大屏幕的高通量样品CytoDYNAMIX Screens™)。

除了我们现有的产品,我们还在产品线中为微管,微管蛋白,运动蛋白,小G蛋白效应物,GAP,GEF和其他几种蛋白提供药物筛选服务。

Cytoskeleton2019年价格表

 

货号 品名   规格   价格     
AAC01 Anti-Acetyl Lysine Mouse Monoclonal Antibody 2 x 100 µl ¥4,295.20
AAC02 Anti-Acetyl Lysine Mouse Monoclonal Antibody 2 x 100 µl ¥4,295.20
AAC02-S Anti-Acetyl Lysine Mouse Monoclonal Antibody (Trial size) 1 x 25 ul ¥1,237.60
AAC03 Anti-Acetyl Lysine Mouse Monoclonal Antibody 2 x 100 µl ¥4,295.20
AAC03-HRP Anti-Acetyl Lysine Mouse Monoclonal Antibody HRP Conjugates 1 x 100 µl ¥5,314.40
AAC03-HRP-S Anti-Acetyl Lysine Mouse Monoclonal Antibody HRP Conjugates (Trial size) 1 x 25 ul ¥1,426.88
AAC03-S Anti-Acetyl Lysine Mouse Monoclonal Antibody (Trial size) 1 x 25 ul ¥1,237.60
AAC04-beads Anti-Acetyl Lysine Mouse Monoclonal Antibody Agarose Conjugates 4 x 500 µl ¥5,751.20
AACX1 Chemically Acetylated Bovine Serum Albumin (BSA) 1 x 500 µg ¥509.60
AAN01-A Anti-actin antibody (rabbit polyAb) + control 1 x 100 µg ¥3,712.80
AAN01-B Anti-actin antibody (rabbit polyAb) + control 3 x 100 µg ¥8,663.20
AB07-A Actin protein (biotin, rabbit skeletal muscle) 5 x 20 µg ¥4,149.60
AB07-C Actin protein (biotin, rabbit skeletal muscle) 20 x 20 µg ¥11,575.20
AB07-D Actin protein (biotin, rabbit skeletal muscle) Large quantities #VALUE!
ACD03 Anti-Cdc42 Mouse Monoclonal Antibody  2 x 200 µl ¥4,295.20
ACD03-S Anti-Cdc42 Mouse Monoclonal Antibody (sample size)  1 x 50 µl ¥1,237.60
ACFL02-A Cofilin antibody (host rabbit) + control 1 x 50 µg ¥4,149.60
ACFL02-B Cofilin antibody (host rabbit) + control 3 x 50 µg ¥10,556.00
AD99-A Actin protein (bovine cardiac muscle, >99% pure) 1 x 1 mg ¥1,441.44
AD99-B Actin protein (bovine cardiac muscle, >99% pure) 5 x 1 mg ¥6,770.40
ADV01-A Advanced Protein Assay (5X conc) 1 x 500 ml ¥2,839.20
ADV01-B Advanced Protein Assay (5X conc) 3 x 500 ml ¥7,498.40
ADV02-A Precision Red Advanced Protein Assay (1X conc) 1 x 500 ml ¥1,820.00
ADV02-B Precision Red Advanced Protein Assay (1X conc) 3 x 500 ml ¥4,732.00
AKF99-A Pre-formed actin filaments (rabbit skeletal muscle) 1 x 1 mg ¥2,475.20
AKF99-B Pre-formed actin filaments (rabbit skeletal muscle) 5 x 1 mg ¥8,663.20
AKL95-B Actin protein (rabbit skeletal muscle, >95% pure) 1 x 1  mg ¥1,237.60
AKL95-C Actin protein (rabbit skeletal muscle, >95% pure) 5 x 1  mg ¥4,877.60
AKL95-XL Actin protein (rabbit skeletal muscle, >95% pure) Large quantities #VALUE!
AKL99-A Actin protein (rabbit skeletal muscle, >99% pure) 4 x 250 µg ¥2,620.80
AKL99-B Actin protein (rabbit skeletal muscle, >99% pure) 2 x 1 mg ¥3,305.12
AKL99-C Actin protein (rabbit skeletal muscle, >99% pure) 5 x 1 mg ¥6,595.68
AKL99-D Actin protein (rabbit skeletal muscle, >99% pure) 10 x 1 mg ¥12,376.00
AKL99-E Actin protein (rabbit skeletal muscle, >99% pure) 20 x 1 mg ¥20,748.00
AKL99-XL Actin protein (rabbit skeletal muscle, >99% pure) Large quantities #VALUE!
AP05-A Actin protein (pyrene, rabbit skeletal muscle) 1 x 1 mg ¥2,839.20
AP05-B Actin protein (pyrene, rabbit skeletal muscle) 5 x 1 mg ¥11,575.20
AP05-XL Actin protein (pyrene, rabbit skeletal muscle) Large quantities #VALUE!
APHL99-A Actin protein (human platelet non-muscle, >99% pure) 2 x 250 µg ¥2,839.20
APHL99-C Actin protein (human platelet non-muscle, >99% pure) 1 x 1 mg ¥4,004.00
APHL99-E Actin protein (human platelet non-muscle, >99% pure) 5 x 1 mg ¥14,487.20
APHL99-XL Actin protein (human platelet non-muscle, >99% pure) Large quantities #VALUE!
APHR-A Actin protein (rhodamine, human platelet non-muscle) 4 x 10 µg ¥3,858.40
APHR-C Actin protein (rhodamine, human platelet non-muscle) 20 x 10 µg ¥12,376.00
APUF01-A Profilin polyclonal antibody (host rabbit) + control 1 x 50 µg ¥3,276.00
APY03 Anti-Phosphotyrosine Mouse Monoclonal Antibody 2 x 100 µl ¥4,295.20
APY03-Beads Anti-Phosphotyrosine Affinity Beads 4 x 300 µl ¥5,314.40
APY03-HRP Anti-Phosphotyrosine-HRP Mouse Monoclonal Antibody 1 x 100 µl ¥5,314.40
APY03-HRP-S Anti-Phosphotyrosine-HRP Mouse Monoclonal Antibody (Trial Size) 1 x 25 µl ¥1,426.88
APY03-HRP-XL Anti-Phosphotyrosine-HRP Mouse Monoclonal Antibody Large Quantities #VALUE!
APY03-S Anti-Phosphotyrosine Mouse Monoclonal Antibody (Trial Size) 1 x 25 µl ¥1,237.60
AR05-B Actin protein (rhodamine, rabbit skeletal muscle) 10 x 20 µg ¥3,858.40
AR05-C Actin protein (rhodamine, rabbit skeletal muscle) 20 x 20 µg ¥7,644.00
ARC03 Anti-Rac1 specific mouse MAb 2 x 100 µl ¥4,295.20
ARC03-S Anti-Rac1 specific mouse MAb(sample size) 1 x 25 µl ¥1,237.60
ARH04 Anti-RhoA antibody (mouse MAb) + control. 2 x 100 µl ¥4,295.20
ARH04-S Anti-RhoA antibody (mouse MAb) + control.  (Trial size) 1 x 50 µl ¥1,237.60
AS99-A Actin protein (chicken gizzard muscle, >99% pure) 1 x 1 mg ¥1,441.44
AS99-B Actin protein (chicken gizzard muscle, >99% pure) 5 x 1 mg ¥6,770.40
ASM23 Anti-SUMO-2/3 Mouse Monoclonal Antibody 2 x 100 µl ¥4,295.20
ASM23-S Anti-SUMO-2/3 Mouse Monoclonal Antibody (Trial Size) 1 x 25 µl ¥1,237.60
ASM24 Anti-SUMO-2/3 Mouse Monoclonal Antibody 2 x 200 µl ¥4,295.20
ASM24-Beads SUMO-2/3 Affinity Beads 2 x 400 µl ¥5,314.40
ASM24-S Anti-SUMO-2/3 Mouse Monoclonal Antibody (Trial Size) 1 x 150 µl ¥1,237.60
AT01-A Alpha-actinin (rabbit skeletal muscle) 2 x 50 µg ¥3,858.40
AT01-C Alpha-actinin (rabbit skeletal muscle) 10 x 50 µg ¥12,885.60
ATN02 Anti-tubulin polyAb pan specific (sheep)  2 x 100 µl ¥4,149.60
ATN02-S Anti-tubulin polyAb pan specific (sheep) (Trial size) 1 x 25 µl ¥1,237.60
AUB01 Anti-Ubiquitin Mouse Monoclonal Antibody 2 x 100 µl ¥4,295.20
AUB01-S Anti-Ubiquitin Mouse Monoclonal Antibody (Trial size) 1 x 25 µl ¥1,237.60
AUB01-XL Anti-Ubiquitin Mouse Monoclonal Antibody 4 x 500 µl ¥9,973.60
BK001 Actin Binding Protein Spin-Down Assay Kit KIT 30-100 assays ¥10,119.20
BK003 Actin Polymerization Biochem Kit  KIT 30-100 assays ¥11,356.80
BK004P Tubulin Polymerization Assay Kit (>97% pure porcine brain tubulin) KIT 24 assays ¥8,663.20
BK005 F-Actin Visualization Kit KIT 300 slides ¥5,168.80
BK006P Tubulin Polymerization Assay Kit (>99% pure porcine brain tubulin) KIT 24 assays ¥12,376.00
BK008 Ras Activation Assay Biochem Kit KIT 50 assays ¥10,177.44
BK008-S Ras Activation Assay Biochem Kit KIT 20 assays ¥5,168.80
BK011P Tubulin Polymerization Assay (fluoresc-96assays porcine brain tubulin) KIT 96 assays ¥12,667.20
BK013 Actin Binding Protein Spin-Down Assay Kit (Non-muscle) KIT 30-100 assays ¥10,628.80
BK029 Microtubule Binding Protein Spin Down Assay Kit KIT 30-100 assays ¥9,682.40
BK030 Combo RhoA/Rac1/Cdc42 Activation Assay Kit KIT 3 x 10 assays ¥12,740.00
BK032-S Arf1 Pulldown Activation Assay Kit KIT 20 assays ¥6,188.00
BK033-S Arf6 Pulldown Activation Assay Kit KIT 20 assays ¥6,188.00
BK034 Cdc42 Pulldown Activation Assay Kit KIT 50 assays ¥10,177.44
BK034-S Cdc42 Pulldown Activation Assay Kit KIT 20 assays ¥5,168.80
BK035 Rac1 Pulldown Activation Assay Kit KIT 50 assays ¥10,177.44
BK035-S Rac1 Pulldown Activation Assay Kit KIT 20 assays ¥5,168.80
BK036 RhoA Pulldown Activation Assay Kit KIT 80 assays ¥10,177.44
BK036-S RhoA Pulldown Activation Assay Kit KIT 20 assays ¥5,168.80
BK037 G-Actin : F-Actin In Vivo Assay Kit KIT 30-100 assays ¥10,119.20
BK038 Microtubule / Tubulin In Vivo Assay Kit KIT 30-100 assays ¥10,119.20
BK040 RalA Activation Assay Biochem Kit KIT 50  assays ¥10,556.00
BK051/52 ATPase ELIPA™ (Enzyme Linked Inorganic Phosphate Assay) KIT 96 assays ¥4,732.00
BK051/52 GTPase ELIPA™ (Enzyme Linked Inorganic Phosphate Assay) KIT 96 assays ¥4,732.00
BK053 HTS Kinesin ATPase Endpoint Assay KIT 1000 assays ¥11,284.00
BK054 CytoPhos™ Phosphate Assay (1-500ug/ml protein reactions) KIT 1000 assays ¥4,732.00
BK060 Kinesin ELIPA (Enzyme Linked Inorganic Phosphate Assay TM) KIT 96 assays ¥10,920.00
BK100 GEF Exchange Assay KIT 60-300 assays ¥9,391.20
BK105 GAP Assay Kit KIT 80-160 assays ¥9,391.20
BK121 RhoA G-LISA Activation Assay (luminescence) KIT 96 assays ¥12,521.60
BK124 RhoA G-LISA Activation Assay (absorbance) KIT 96 assays ¥12,521.60
BK124-S RhoA G-LISA Activation Assay (absorbance) KIT 24 assays ¥7,207.20
BK124-XL RhoA G-LISA Activation Assay (absorbance) Large Quantities #VALUE!
BK125 Rac1,2,3 G-LISA Activation Assay Kit KIT 96 assays ¥12,521.60
BK126 Rac1 G-LISA Activation Assay Kit KIT 96 assays ¥12,521.60
BK127 Cdc42 G-LISA Activation Assay Kit KIT 96 assays ¥12,521.60
BK127-S Cdc42 G-LISA Activation Assay Kit KIT 24 assays ¥7,207.20
BK128 Rac1 G-LISA Activation Assay Kit KIT 96 assays ¥12,521.60
BK128-S Rac1 G-LISA Activation Assay Kit KIT 24 assays ¥7,207.20
BK129 RalA G-LISA Activation Assay Kit KIT 96 assays ¥12,521.60
BK131 Ras G-LISA Activation Assay Kit KIT 96 assays ¥12,521.60
BK131-XL Ras G-LISA Activation Assay Kit Large Quantities #VALUE!
BK132 Arf1 G-LISA Activation Assay Kit KIT 96 assays ¥12,521.60
BK133 Arf6 G-LISA Activation Assay Kit KIT 96 assays ¥12,521.60
BK135 G-LISA Bundle (BK135 = BK124-S + BK127-S + BK128-S) 3 KITS (24 assays/kit) ¥20,238.40
BK150 RhoA ELISA Kit KIT 96 assays ¥6,770.40
BK160 Signal-Seeker™ Phosphotyrosine Enrichment Kit (30 assays) KIT 30 assays ¥7,265.44
BK160-S Signal-Seeker™ Phosphotyrosine Enrichment Kit (10 assays) KIT 10 assays ¥2,839.20
BK161 Signal-Seeker™ Ubiquitin Enrichment Kit (30 assays) KIT 30 assays ¥7,265.44
BK161-S Signal-Seeker™ Ubiquitin Enrichment Kit (10 assays) KIT 10 assays ¥2,839.20
BK162 Signal-Seeker™ SUMOylation Enrichment Kit (30 assays) KIT 30 assays ¥7,265.44
BK162-S Signal-Seeker™ SUMOylation Enrichment Kit (10 assays) KIT 10 assays ¥2,839.20
BK163 (30 assays) Signal-Seeker™ Acetyl-Lysine Enrichment Kit (30 assays) KIT 30 assays ¥7,207.20
BK163-S (10 assays) Signal-Seeker™ Acetyl-Lysine Enrichment Kit (10 assays) KIT 10 assays ¥2,839.20
BLR01 BlastR Rapid Lysate Prep Kit 50 purifications ¥2,693.60
BLR02 BlastR Rapid Filtration Kit 50 purifications ¥1,747.20
BM01-A BimC Motor Domain (Asp.nid.) 2 x 25 µg ¥4,732.00
BS01 GTP gamma S 1 x 500 µg ¥1,354.08
BSA01-001 General Actin Buffer 1 x 10 ml ¥990.08
BSA01-010 General Actin Buffer 1 x 100 ml ¥2,169.44
BSA02-001 Actin Polymerization Buffer (10x stock) 1 x 2 ml ¥990.08
BSA04-001 ATP (100mM Stock) 1 x 1 ml ¥990.08
BST01-001 General Tubulin Buffer 1 x 10 ml ¥990.08
BST01-010 General Tubulin Buffer 1 x 100 ml ¥2,169.44
BST05-001 Tubulin Glycerol Buffer 1 x 10 ml ¥990.08
BST06-001 GTP (100mM Stock) 1 x 100 µl ¥990.08
BST06-010 GTP (100mM Stock) 10 x 100 µl ¥2,984.80
C17G01-A Cdc42-GST protein: dominant negative 1 x 25 µg  ¥3,756.48
C6101-A Cdc42-His Protein: constituitively active 1 x 10 µg ¥3,756.48
CD01-A Cdc42-His Protein: Wild type 1 x 100 µg ¥3,756.48
CD01-C Cdc42-His Protein: Wild type 3 x 100 µg ¥9,143.68
CD01-XL Cdc42-His Protein: Wild type 1 x 1 mg ¥19,350.24
CDG01-C Cdc42-GST Protein: Wild type 8 x 25 µg ¥7,017.92
CF01-A Cofilin (Human Recombinant) 1 x 100 µg ¥3,523.52
CF01-C Cofilin (Human Recombinant) 4 x 100 µg ¥11,327.68
CIG01-beads Control for Immunoprecipitation IgG beads 10 assays ¥1,237.60
CN01-A Rho Activator 5 x 10 units ¥2,897.44
CN01-B Rho Activator 20 x 10 units ¥10,556.00
CN02-A Rac and Cdc42 Activator 5 x 10 units ¥2,897.44
CN02-B Rac and Cdc42 Activator 20 x 10 units ¥10,556.00
CN03-A Rho activator II 3 x 20 µg ¥2,897.44
CN03-B Rho activator II 9 x 20 µg ¥8,153.60
CN03-XL Rho activator II Large Quantities #VALUE!
CN04-A Rho/Rac/Cdc42 Activator I 3 x 20 µg ¥2,897.44
CN04-B Rho/Rac/Cdc42 Activator I 9 x 20 µg ¥8,153.60
CP01-A CENP-E Motor domain (Hm.sap.) 2 x 25 µg ¥4,732.00
CP01-XL CENP-E Motor domain (Hm.sap.) (Bulk discount avail.) 1 x 1 mg ¥24,024.00
CR01-A Chromokinesin Motor Domain (Hm.sap.) 2 x 25 µg ¥4,732.00
CS-DN01 Dynein (Cytoplasmic), Porcine brain 1 x 50 µg ¥5,678.40
CS-GE02 SOS1 Ras GEF protein (human recomb. ExD domain, 6xHis tagged) 1 x 100 µg ¥4,732.00
CS-GE02-XL SOS1 Ras GEF protein (human recomb. ExD domain, 6xHis tagged) 1 x 1 mg ¥24,024.00
CS-GE03 Ras-GRF GEF protein (human recomb. CDC25 domain, MBP tagged) 1 x 100 µg ¥4,732.00
CS-GE04 Tiam1 GEF protein (human recomb. DHPH domain, MBP tagged) 1 x 100 µg ¥4,732.00
CS-GE05 Vav1 GEF protein (human recomb. DHPHC1 domain Y174D mutant, 6xHis tagged) 1 x 100 µg ¥4,732.00
CS-GE06 Vav2 GEF protein (human recomb. DH domain, 6xHis tagged) 1 x 100 µg ¥4,732.00
CS-GE07 ARNO Protein: GEF (Sec7) domain (aa31- 267, human recomb., 6xHis tagged) 1 x 100 µg ¥4,732.00
CS-H001-B Hela Cancer Cell Tubulin 1 x 250 µg ¥11,618.88
CS-H005 MCF-7 Cancer Cell Tubulin 1 x 250 µg ¥11,618.88
CS-KF51 KIF7 motor domain (human recombinant) 1 x 100 µg ¥4,732.00
CS-MH03 Heavy Meromyosin Protein (HMM Fragment) 1 x 100 µg ¥4,295.20
CS-MH03-XL Heavy Meromyosin Protein (HMM Fragment) Large quantities #VALUE!
CS-MP05 MKLP2 motor domain (human recombinant) 1 x 50 µg ¥5,620.16
CS-MYS02 Myosin S1 Fragment (Skeletal muscle), Bovine 1 x 250 µg ¥3,756.48
CS-MYS03 Myosin S1 Fragment (Cardiac), Bovine cardiac 1 x 250 µg ¥3,756.48
CS-MYS03-XL Myosin S1 Fragment (Cardiac), Bovine cardiac Large Quantities #VALUE!
CS-MYS04 Myosin S1 Fragment (Skeletal), Rabbit skeletal 1 x 250 µg ¥3,756.48
CS-MYS05 Myosin S1 Fragment (smooth), Chicken gizzard 1 x 250 µg ¥3,756.48
CS-RS03 K-Ras4B Protein: wild-type (human recombinant) 1 x 100 µg ¥5,474.56
CS-TFC01 Actin Thin Filaments (Bovine cardiac) 1 x 1 mg ¥8,736.00
CS-TM001 Caki-1 tumor tubulin 1 x 250 µg #VALUE!
CS-TT05 Tropomyosin / Tropomodulin Complex, Bovine cardiac 1 x 1 mg ¥4,004.00
CT03-A C3 transferase protein 1 x 25 µg ¥2,897.44
CT03-C C3 transferase protein 4 x 25 µg ¥9,973.60
CT04-A Cell Permeable Rho Inhibitor (C3 Trans based)) 1 x 20 µg ¥2,897.44
CT04-B Cell Permeable Rho Inhibitor (C3 Trans based)) 5 x 20 µg ¥8,663.20
CT04-C Cell Permeable Rho Inhibitor (C3 Trans based)) 20 x 20 µg ¥22,932.00
CT04-XL Cell Permeable Rho Inhibitor (C3 Trans based)) Large Quantities #VALUE!
CUB01 Control for Ubiquitin Affinity Beads 10 assays ¥1,237.60
CY-SC001 SiR-Actin Kit 50-300 slides ¥7,265.44
CY-SC001-XL SiR-Actin Kit Large Quantities #VALUE!
CY-SC002 SiR-Tubulin Kit 50-300 slides ¥7,265.44
CY-SC006 Cytoskeleton Kit (SiR-Actin + SiR-Tubulin) 50-300 slides each ¥12,012.00
CY-SC007 SiR-DNA Kit 50-300 slides ¥4,295.20
CY-SC012 SiR-Lysosome Kit 50-300 slides ¥5,460.00
CY-SC013 SiR700-Actin Kit 35-200 slides ¥7,265.44
CY-SC014 SiR700-Tubulin Kit 35-200 slides ¥7,265.44
CY-SC015 SiR700-DNA Kit 35-200 slides ¥4,295.20
CY-SC016 SiR700-Lysosome Kit 35-200 slides ¥5,460.00
EG01-A Eg5 Motor Domain (Hm.sap.) 2 x 25 µg ¥4,732.00
EG01-B Eg5 Motor Domain (Hm.sap.) 10 x 25 µg ¥14,924.00
EG01-XL Eg5 Motor Domain (Hm.sap.) (Bulk discount avail.) 1 x 1 mg ¥24,024.00
EG02-A Eg5 Homolog Motor Domain (Asp.fum.) 2 x 15 µg ¥4,732.00
ELP03 Purine Nucleoside Phosphorylase (PNP, 96 assays) 1 x 50 U ¥2,839.20
ELP03-XL Purine Nucleoside Phosphorylase (PNP) Large Quantities #VALUE!
FNR01-A Fibronectin Rhodamine (bovine plasma) 5 x 20 µg ¥4,732.00
FNR01-B Fibronectin Rhodamine (bovine plasma) 20 x 20 µg ¥13,468.00
FNR01-XL Fibronectin Rhodamine (bovine plasma) Large Quantities #VALUE!
FNR02-A Fibronectin HiLyte488™ (bovine plasma) 5 x 20 µg ¥4,732.00
FNR02-B Fibronectin HiLyte488™ (bovine plasma) 20 x 20 µg ¥13,468.00
FNR03-A Fibronectin Biotin (bovine plasma) 5 x 20 µg ¥4,732.00
FNR03-B Fibronectin Biotin (bovine plasma) 20 x 20 µg ¥13,468.00
FTZ02-A FtsZ Protein (Staphylococcus aureus recombinant plus 6xHis tag) 1 x 1 mg ¥5,241.60
FTZ02-B FtsZ Protein (Staphylococcus aureus recombinant plus 6xHis tag) 5 x 1 mg ¥23,296.00
FTZ02-XL FtsZ Protein (Staphylococcus aureus recombinant plus 6xHis tag) Large Quantities #VALUE!
FTZ03-A FtsZ Protein (Streptococcus pneumoniae recombinant plus 6xHis tag) 1 x 1 mg ¥5,241.60
FTZ03-B FtsZ Protein (Streptococcus pneumoniae recombinant plus 6xHis tag) 5 x 1 mg ¥23,296.00
FTZ04-A FtsZ Protein (Enterococcus faecalis recombinant plus 6xHis tag) 1 x 1 mg ¥5,241.60
FTZ04-B FtsZ Protein (Enterococcus faecalis recombinant plus 6xHis tag) 5 x 1 mg ¥23,296.00
FTZ05-A FtsZ Protein (E.coli  recombinant, 6xHis-tagged) 1 x 1 mg ¥5,241.60
FTZ05-B FtsZ Protein (E.coli  recombinant, 6xHis-tagged) 5 x 1 mg ¥23,296.00
GAP01-A Rho GAP Protein GST 1 x 50 µg ¥3,669.12
GAP01-B Rho GAP Protein GST 4 x 50 µg ¥9,682.40
GAS01-A Rho GAP Protein: catalytic domain GST 1 x 50 µg ¥3,669.12
GAS01-B Rho GAP Protein: catalytic domain GST 4 x 50 µg ¥9,682.40
GDI01-A GDP dissociation inhibitor protein GST fusion 1 x 25 µg ¥3,669.12
GE01-A Dbs protein GEF domain – HIS fusion 2 x 50 µg ¥3,669.12
GGA05-A GGA3-PBD Arf Affinity Beads 1 x 500 µg ¥5,751.20
H003 Hela Cancer Cell Tubulin Biotinylated 1 x 40 µg ¥9,536.80
HPG6-A Gelsolin (recomb. Human) 4 x 20 µg ¥4,295.20
HPG6-B Gelsolin (recomb. Human) 20 x 20 µg ¥14,487.20
HTS03-A Tubulin (HTS format, porcine, 8 assays) 1 x 4 mg ¥4,804.80
HTS03-B Tubulin (HTS format, porcine, 96 assays) 1 x 40 mg ¥23,281.44
KC01-A KIFC3 Motor Domain (Hm.sap) 2 x 25 µg ¥4,732.00
KF01-A KIF3C Motor Domain (Hm.sap.) 2 x 25 µg ¥4,732.00
KR01-A KHC Motor Domain (Hm.sap.) 2 x 25 µg ¥4,732.00
KR01-XL KHC Motor Domain (Hm.sap.) (Bulk discounts avail.) 1 x 1 mg ¥24,024.00
LMN01-A Laminin Rhodamine (mouse tumor) 5 x 20 µg ¥4,732.00
LMN01-B Laminin Rhodamine (mouse tumor) 20 x 20 µg ¥13,468.00
LMN02-A Laminin HiLyte488™ (mouse tumor) 5 x 20 µg ¥4,732.00
LMN02-B Laminin HiLyte488™ (mouse tumor) 20 x 20 µg ¥13,468.00
LMN02-XL Laminin HiLyte488™ (mouse tumor) Large Quantities #VALUE!
LMN03-A Laminin Biotin (mouse tumor) 5 x 20 µg ¥4,732.00
LMN03-B Laminin Biotin (mouse tumor) 20 x 20 µg ¥13,468.00
MAPF-A MAP Fraction 1 x 100 µg ¥3,276.00
MAPF-C MAP Fraction 5 x 100 µg ¥11,575.20
MH01-A Myosin: Heavy Meromyosin 4 x 50 µg ¥3,756.48
MK01-A MCAK Motor Domain (Hm.sap.) 2 x 25 µg ¥4,732.00
ML116-A Tubulin: MAP-rich (porcine) 1 x 1 mg ¥1,441.44
ML116-B Tubulin: MAP-rich (porcine) 5 x 1 mg ¥6,770.40
ML116-DX Tubulin: MAP-rich (porcine) 1 x 10 mg ¥11,429.60
ML116-XL Tubulin: MAP-rich (porcine) Large quantities #VALUE!
MP01-A MKLP Motor Domain (Hm.sap.) 2 x 25 µg ¥4,732.00
MP01-XL MKLP Motor Domain (Hm.sap.)  1 x 1 mg ¥24,024.00
MT002-A Microtubules Pre-formed Motor ATPase ready(porcine) 4 x 500 µg ¥5,460.00
MT002-XL Microtubules Pre-formed Motor ATPase ready(porcine) 1 x 10 mg ¥18,200.00
MY02-A Myosin Skeletal Muscle 5 x 1 mg ¥3,276.00
MY02-B Myosin Skeletal Muscle 20 x 1 mg ¥11,284.00
MY03-A Myosin Cardiac Muscle 5 x 1 mg ¥3,276.00
MY03-B Myosin Cardiac Muscle 20 x 1 mg ¥11,284.00
PAK01-A PAK Rac1/Cdc42 binding domain protein 1 x 250 µg ¥5,314.40
PAK01-B PAK Rac1/Cdc42 binding domain protein 4 x 250 µg ¥14,487.20
PAK02-A PAK Beads, Rac1/Cdc42 binding domain protein on beads 1 x 500 µg ¥6,188.00
PAK02-B PAK Beads, Rac1/Cdc42 binding domain protein on beads 4 x 500 µg ¥19,364.80
PHDG1-A Acti-stain™ 488 phalloidin 1 x 500 µl (300 slides) ¥2,722.72
PHDH1-A Acti-stain™ 555 phalloidin 1 x 500 µl (300 slides) ¥2,722.72
PHDN1-A Acti-stain™ 670 phalloidin 1 x 500 µl (300 slides) ¥2,722.72
PHDR1 Rhodamine Phalloidin (14 µM in methanol) 1 x 500 µl ¥2,722.72
PIC02 Protease Inhibitor Cocktail (100x strength) 1 x 1 ml ¥990.08
PR02-A Profilin 1 (recombinant human no tag) 1 x 100 µg ¥2,548.00
PR02-B Profilin 1 (recombinant human no tag) 1 x 500 µg ¥5,460.00
PR02-XL2 Profilin 1 (recombinant human no tag) 1 x 1 mg ¥10,920.00
R17G01-A Rac1 GST Protein: dominant negative 1 x 25 µg ¥3,756.48
R6101-A Rac1 His Protein: constitutively active 1 x 10 µg ¥3,756.48
R6301-A RhoA His Protein: constitutively active 1 x 10 µg ¥3,756.48
RC01-A Rac1 His Protein: wild type 1 x 100 µg ¥3,756.48
RC01-C Rac1 His Protein: wild type 3 x 100 µg ¥9,376.64
RC01-XL Rac1 His Protein: wild type 1 x 1 mg ¥19,350.24
RC02-A Rac2 His Protein: wild type 1 x 100 µg ¥3,756.48
RCG01-C Rac1 GST Protein: wild type 8 x 25 µg ¥6,755.84
RF02-A Raf protein ras binding domain on GST beads 1 x 2 mg ¥6,595.68
RF02-B Raf protein ras binding domain on GST beads 4 x 2 mg ¥19,423.04
RH01-A RhoA His Protein: wild type 1 x 100 µg ¥3,756.48
RH01-C RhoA His Protein: wild type 3 x 100 µg ¥9,376.64
RH01-XL RhoA His Protein: wild type 1 x 1 mg ¥19,350.24
RH03-A RhoC His Protein: wild type 1 x 100 µg ¥3,756.48
RHG01-C RhoA GST Protein: wild type 8 x 25 µg ¥6,755.84
RP01P-A Arp2/3 Protein Complex (Porcine brain) 2 x 50 µg ¥5,605.60
RP01P-B Arp2/3 Protein Complex (Porcine brain) 6 x 50 µg ¥14,487.20
RR02-A Rap1b protein (Human wild type, His) 1 x 100 µg ¥3,756.48
RS01-A H-Ras-His Protein wild type 1 x 100 µg ¥3,756.48
RS01-C H-Ras-His Protein wild type 3 x 100 µg ¥9,376.64
RT01-A Rhotekin RBD protein GST fusion 1 x 500 µg ¥5,809.44
RT02-A Rhotekin RBD protein on GST beads 2 x 2 mg ¥7,935.20
RT02-B Rhotekin RBD protein on GST beads 6 x 2 mg ¥18,855.20
T238P-A Tubulin (porcine brain, >99% pure) 1 x 1 mg ¥2,256.80
T238P-B Tubulin (porcine brain, >99% pure) 5 x 1 mg ¥8,590.40
T238P-C Tubulin (porcine brain, >99% pure) 20 x 1 mg ¥25,407.20
T240-A Tubulin (porcine brain, >99% pure) 1 x 1 mg ¥1,601.60
T240-B Tubulin (porcine brain, >99% pure) 5 x 1 mg ¥7,644.00
T240-C Tubulin (porcine brain, >99% pure) 20 x 1 mg ¥25,261.60
T240-DX Tubulin (porcine brain, >99% pure) 1 x 10 mg ¥12,376.00
T240-XL Tubulin (porcine brain, >99% pure) Large Quantities #VALUE!
T333P-A Tubulin (Biotin Conjugate; Porcine Brain) 5 x 20 µg ¥4,295.20
T333P-B Tubulin (Biotin Conjugate; Porcine Brain) 20 x 20 µg ¥11,575.20
T333P-XL Tubulin (Biotin Conjugate; Porcine Brain) 1 x 500 µg ¥9,973.60
TA01-A Tau (bovine brain) 1 x 50 µg ¥4,353.44
TA01-B Tau (bovine brain) 3 x 50 µg ¥9,755.20
TL440M-A Tubulin (AMCA dye labeled; porcine, micro-injctn) 5 x 20 µg ¥4,295.20
TL440M-B Tubulin (AMCA dye labeled; porcine, micro-injctn) 20 x 20 µg ¥11,575.20
TL488M-A Tubulin (HiLyte 488TM dye labeled; porcine, micro-injctn) 5 x 20 µg ¥4,295.20
TL488M-B Tubulin (HiLyte 488TM dye labeled; porcine, micro-injctn) 20 x 20 µg ¥11,575.20
TL590M-A Tubulin (Rhodamine labeled; porcine, micro-injctn) 5 x 20 µg ¥4,295.20
TL590M-B Tubulin (Rhodamine labeled; porcine, micro-injctn) 20 x 20 µg ¥11,575.20
TL620M-A Tubulin (X-rhodamine labeled; Bovine Brain micr-injctn) 5 x 20 µg ¥4,295.20
TL620M-B Tubulin (X-rhodamine labeled; Bovine Brain micr-injctn) 20 x 20 µg ¥11,575.20
TL670M-A Tubulin (HiLyte 647TM dye labeled; porcine, micro-injctn) 5 x 20 µg ¥4,295.20
TL670M-B Tubulin (HiLyte 647TM dye labeled; porcine, micro-injctn) 20 x 20 µg ¥11,575.20
TXD01 Paclitaxel  (2mM in DMSO) 10 x 100 µl ¥2,548.00
UBA01-beads Ubiquitin Affinity Beads 40 rxs ¥5,620.16
V01-A Vimentin (Recombinant Syrian Hamster) 2 x 50 µg ¥2,475.20
V01-C Vimentin (Recombinant Syrian Hamster) 10 x 50 µg ¥11,575.20
VCG03-A VCA domain of WASP (GST fusion) 1 x 500 µg ¥6,377.28
WBM01 GOBlot Western Blot Processor each ¥11,575.20
WBM01-XL GOBlot Western Blot Processor four pack ¥40,040.00

 

 

 

 

Cytoskeleton ACFL02-A说明书

 

Cytoskeleton,Inc。很高兴为药物筛选,信号转导和细胞骨架研究提供广泛的试剂盒和产品。我们专注于生产纯化蛋白质和易于使用的试剂盒,以研究生物化学和细胞过程。我们的试剂盒可用于少量样品,用于基础研究或小屏幕(Biochem Kits™),也可用于大屏幕的高通量样品CytoDYNAMIX Screens™)。

除了我们现有的产品,我们还在产品线中为微管,微管蛋白,运动蛋白,小G蛋白效应物,GAP,GEF和其他几种蛋白提供药物筛选服务。

 

Anti-cofilin: rabbit polyclonal

抗cofilin:兔多克隆

 

窗体顶端

1×50 ug  (Cat. # ACFL02-A)

3×50 ug  (Cat. # ACFL02-B

产品用途
该抗体推荐用于检测人,小鼠,大鼠和非洲爪蟾中的cofilin(图2)。以下方案已使用此抗体进行了测试:  

 

 

蛋白质印迹

免疫组化

ELISA

免疫沉淀

 

没有

 

材料
ACFL02作为亲和纯化的兔多克隆抗体提供。Cofilin是肌动蛋白结合蛋白,其通过单体结合和切断F-肌动蛋白来调节肌动蛋白动力学和重组(综述于Bamburg.1999.Annu Rev Cell Dev Biol.14:185-230)。针对人cofilin1的N末端13-22个氨基酸产生抗体。天然的cofilin具有19kD的预测分子量,并在21kD的SDS-PAGE凝胶上迁移(Yonezawa等,1987; Cell Structure and Function 12:443-452)。血小板细胞额外包括ct(1mg; Cat。#EXT01)作为Western印迹阳性对照(参见图1)。ACFL02以冻干白色粉末形式提供。  

 

图1.抗 – cofilin抗体(Cat。#ACFL02)的Western印迹分析。 通过电泳分离蛋白质样品,并如方法中所述转移至PVDF膜。ACFL02抗体w稀释至500ng / ml(1:500)用于Western印迹分析。在20μg血小板细胞提取物中检测到Cofilin(参见箭头)。分子量标记来自Invitrogen。  
 

 

图2.用抗 – cofilin抗体(Cat。#ACFL02)探测的细胞提取物的Western印迹。化学发光检测50微克huma中的cofilin(见箭头)n HeLa细胞提取物(泳道5)。印迹上显示的其他泳道是果蝇S2(泳道1),非洲爪蟾A6(泳道2),小鼠Swiss 3T3(泳道3),大鼠NRK(泳道4)和人血小板(泳道6)细胞提取物(各50μg)。 。分子量标记来自Invitrogen。   

 

图3.用抗 – cofilin抗体(Cat。#ACFL02)染色的小鼠Swiss 3T3细胞的免疫荧光图像。使Swiss Swiss 3T3细胞生长至半融合并用3.7%甲醛固定。显示了使用500ng / ml(1:250稀释)ACFL02抗体的免疫荧光染色(红色)。用1:500稀释的山羊抗兔罗丹明缀合的抗体(Cat。#RG05)检测抗体。用PBS中的100nM DAPI染色DNA(蓝色)。照片是用20倍物镜拍摄的。

 

问题1:抗cofilin抗体(Cat。#ACFL02)产生的抗原是什么?

答案1:针对人cofilin1的N末端13-22个氨基酸产生抗体。

 

问题2:哪些物种的抗cofilin抗体(Cat。#ACFL02)经过测试?

答案2:  建议将抗体用于检测人,小鼠,大鼠和非洲爪蟾细胞裂解液中的cofilin 。

 

 

 

J.Neurosci。RES。2015年; doi:10.1002 / jnr.23602。
“Rho GTPases的N-钙粘蛋白水平和下游效应物与大鼠海马神经元慢性应激诱导的树突棘损失的关联”
作者:CastañedaP。等。
见PubMed文章

PLoS One。2014; 9,e94787。
“活动依赖性树突棘收缩和生长涉及通过不同机制下调cofilin。” 
作者:B。Calabrese等。
见PubMed文章

上午。J. Physiol。肺细胞。摩尔。生理学。2013; 305,L651-64。
“凝血酶选择性地参与LIM激酶1和弹弓-1L磷酸酶以调节NF-κB活化和内皮细胞炎症”
作者:Leonard A.等。
见PubMed文章

Neurotox。RES。2013; 24,532-548。
“慢性酒精改变原代培养神经元中树突棘的发育”
作者:Romero AM等。
见PubMed文章

PLoS One。2012; 7,e41342。
“活性氧通过控制肌动蛋白动力学来调节突出效率。”
作者:N。Taulet等。
见PubMed文章

J Neurosci。2012; 32,3697-711。
“精神分裂症易感因子dysbindin的定量蛋白质组学和遗传学分析鉴定了溶酶体相关细胞器复合物生物发生的新作用1.”
作者:A。Gokhale等。
见PubMed文章

J Neurosci。2010; 30,10977-84。
“突触Rac的生理活化> PAK p-21激活的激酶信号在脆性X综合征的小鼠模型中是有缺陷的。”
作者:LY Chen等。
见PubMed文章

J Neurosci。2009年; 29,8129-42。
“粘着激酶作用于EphB受体的下游,通过调节cofilin活性来维持成熟的树突棘。”
作者:Y。Shi等。
见PubMed文章

进出口。Cell Res。2005; 302,83-95。
“细胞迁移中的膜褶皱:低效的lamellipodia粘附指标和肌动蛋白丝重组的区室。” 
作者:B。Borm等。
见PubMed文章

纳特。细胞生物学。2005; 7,21-29。
“Chronophin是一种新型HAD型丝氨酸蛋白磷酸酶,可调节cofilin依赖性肌动蛋白动力学。” 
作者:A。Gohla等。
见PubMed文章

J. Cell Biol。2005; 168,619-631。
“没有lamellipodium的细胞迁移:肌动蛋白动力学转化为原肌球蛋白介导的细胞运动。” 
作者:SL Gupton等。
见PubMed文章

进出口。Cell Res。2005; 307,342-353。
“亚汇合的内皮细胞在细胞因子和RhoGTP酶信号传导下游形成小体。” 
作者:AE Osiak等。
见PubMed文章

摩尔。细胞。生物学。2005; 25,5119-5133。
“MEK1脚手架蛋白MP1通过整合PAK1和Rho信号来调节细胞扩散。” 
作者:A。Pullikuth等。
见PubMed文章

J. Biol。化学。2004; 279,14039-14048。
“N末端对于原肌球蛋白功能至关重要:N末端修饰会破坏应激纤维的组织,并消除原肌球蛋白-1的抗致癌作用。” 
作者:S。Bharadwaj等。
见PubMed文章

摩尔。生物学。Cell 2004; 15,2639-2651。
“肌动蛋白丝转换和肌球蛋白II运动在细胞骨架驱动的上皮顶端交界复合体的解体中的作用。” 
作者:AI Ivanov等。
见PubMed文章

J. Biol。化学。2004; 279,1885-1891。
“细胞质p21Cip1参与Ras诱导的ROCK / LIMK / cofilin通路的抑制。” 
作者:S。Lee和DM Helfman。
见PubMed文章

EMBO J. 2004; 23,3973-3983。
“稳定F-肌动蛋白的突变肌动蛋白使用不同的机制激活SRF共激活因子MAL。” 
作者:G。Posern等。
见PubMed文章

摩尔。细胞。生物学。2004; 24,9371-9382。
“通过分析激素抗性细胞,Cofilin 1被揭示为糖皮质激素受体的抑制剂。” 
作者:J。Ruegg等。
见PubMed文章

摩尔。生物学。Cell 2004; 15,5369-5382。
“内溶酶体分选机制与中间细胞细胞骨架相互作用。” 
作者:ML Styers等。
见PubMed文章

纳特。细胞生物学。2003; 5,1083-1089。
“cofilin的线粒体易位是凋亡诱导的早期步骤。” 
作者:BT Chua等。
见PubMed文章

J. Biol。化学。2003; 278,40169-40176。
“Cofilin与ClC-5相互作用并调节近端小管细胞系中的白蛋白摄取。” 
作者:DH Hryciw等。
见PubMed文章

癌症研究 2003; 63,6405-6412。
“血管生成抑制剂通过改变热休克蛋白27和cofilin的调节来靶向内皮细胞细胞骨架。” 
作者:SM Keezer等。
见PubMed文章

癌症研究 2003; 63,4641-4647。
“表达稳定的β-连环蛋白的肠上皮细胞的蛋白质组学分析。” 
作者:M。Seike等。
见PubMed文章

J. Biol。化学。2003; 278,13578-13584。
“RhoA和ROCK通过限制膜突起促进迁移。” 
作者:RA Worthylake和K. Burridge。
见PubMed文章

摩尔。生物学。Cell 2002; 13,2946-2962。
“PI-3激酶与PAK1的结合导致肌动蛋白磷酸化和细胞骨架重组。” 
作者:EA Papakonstanti和C. Stournaras。
见PubMed文章

摩尔。生物学。Cell 2002; 13,336-347。
“通过MEK依赖性途径对ROCKI / Rho激酶的转录后下调导致Ras转化的成纤维细胞中的细胞骨架破坏。” 
作者:G。Pawlak和DM Helfman。
见PubMed文章

J. Biol。化学。2001; 276,23092-23096。
“通过肌强直性营养不良激酶相关的Cdc42结合激酶α激活LIM激酶。” 
作者:T。Sumi等。
见PubMed文章

 

抗体

· 概述

· 常见问题解答

Cytoskeleton Inc致力于为肌动蛋白和肌动蛋白结合蛋白提供高质量的抗体。我们在该系列产品中的所有抗体均经过QC Max™测试。已经测试QC Max TM抗体用于蛋白质印迹,原位和免疫沉淀。这样,您就可以准确了解每种抗体的用途,并确保购买适合您的抗体。如果您对我们的QC Max™抗体有关的详细信息,请点击下面列出的产品以获取更多详细信息。

问题1:在细胞和组织中染色F-肌动蛋白的*方法是什么?

答案2:   细胞骨架提供我们的Acti染色系列荧光标记的鬼笔环肽,可在固定细胞和组织样本中特异性染色F-肌动蛋白。Acti染色荧光鬼笔环肽探针非常明亮和稳定,为标记F-肌动蛋白提供了一种非常简单和经济的方法。鬼笔环肽用不同的绿色和红色荧光团(Cat。#PHDG1PHDR1PHDH1PHDN1标记。也可以使用肌动蛋白抗体,但通常那些也染色G-肌动蛋白,这产生高背景。

 

问题2:您对多克隆抗肌动蛋白抗体有何推荐稀释度?

答案2:   抗体的稀释度取决于所使用的细胞/组织类型和实验形式。对于蛋白质印迹,我们已经测试并推荐使用浓度为500ng / ml(1:1000稀释)的抗体。在该稀释度下,我们在非洲爪蟾 A6细胞,小鼠Swiss 3T3细胞,大鼠NRK细胞,人HeLa细胞和血小板细胞的细胞提取物中检测到肌动蛋白。对于免疫荧光,我们使用抗肌动蛋白抗体检测小鼠Swiss 3T3细胞中的肌动蛋白。抗体以2μg/ ml(1:500稀释)的浓度使用,然后与1:500稀释的抗兔罗丹明缀合的第二抗体一起温育。这些信息通常在图例中或我们的抗体数据表的“产品用途”部分中找到。

 

 

 

货号

品名

规格

品牌

BK165-S

Signal-Seeker™ SUMOylation 1 Detection Kit

(10 assay)

cytoskeleton

BK165

Signal-Seeker™ SUMOylation 1 Detection Kit

(30 assay)

cytoskeleton

BK163-S

Signal-Seeker™ Acetyl-Lysine Detection Kit

(10 assay)

cytoskeleton

BK163

Signal-Seeker™ Acetyl-Lysine Detection Kit

(30 assay)

cytoskeleton

BK160-S

Signal-Seeker™ Phosphotyrosine Detection Kit

(10 assay)

cytoskeleton

BK160

Signal-Seeker™ Phosphotyrosine Detection Kit

(30 assay)

cytoskeleton

BK162-S

Signal-Seeker™ SUMOylation 2/3 Detection Kit

(10 assay)

cytoskeleton

BK162

Signal-Seeker™ SUMOylation 2/3 Detection Kit

(30 assay)

cytoskeleton

BK161-S

Signal-Seeker™ Ubiquitination Detection Kit

(10 assay)

cytoskeleton

BK161

Signal-Seeker™ Ubiquitination Detection Kit

(30 assay)

cytoskeleton

AAC04-Beads

Acetyl-Lysine Affinity Beads

ea

cytoskeleton

APY03-Beads

Anti-Phosphotyrosine Affinity Beads

ea

cytoskeleton

ASM11-Beads

SUMOylation 1 Affinity Beads

ea

cytoskeleton

ASM24-Beads

SUMOylation 2/3 Affinity Beads

ea

cytoskeleton

UBA01-Beads

Ubiquitination Affinity Beads

ea

cytoskeleton

CIG02-Beads

Acetyl-Lysine Control Beads

ea

cytoskeleton

CIG03-Beads

SUMO1 IP Control Beads

ea

cytoskeleton

CIG01-Beads

IgG Control Beads

ea

cytoskeleton

CUB02-Beads

Ubiquitination Control Beads

ea

cytoskeleton

BLR01

BlastR™ Rapid Lysate Prep Kit

ea

cytoskeleton

BLR02

BlastR™ Rapid Filtration Kit

ea

cytoskeleton

AAC02

Acetyl Lysine Antibody Mouse Monoclonal (7B1)

ea

cytoskeleton

AAC03

Acetyl Lysine Antibody Mouse Monoclonal (19C4B2.1)

ea

cytoskeleton

AAC03-HRP

Acetyl Lysine-HRP Antibody Mouse Monoclonal (19C4B2.1)

ea

cytoskeleton

AAC01

Acetyl Lysine Antibody Mouse Monoclonal (3C6.08.20)

ea

cytoskeleton

APY03

Phosphotyrosine Antibody Mouse Monoclonal (27B10)

ea

cytoskeleton

APY03-HRP

Phosphotyrosine-HRP Antibody Mouse Monoclonal (27B10)

ea

cytoskeleton

ASM01

SUMO1 Antibody Mouse Monoclonal (Clone 5D8B16)

ea

cytoskeleton

ASM24

SUMO-2/3 Antibody Mouse Monoclonal (Clone 11G2)

ea

cytoskeleton

ASM23

SUMO-2/3 Antibody Mouse Monoclonal (Clone 12F3)

ea

cytoskeleton

ASM23-HRP

SUMO-2/3-HRP Antibody Mouse Monoclonal (Clone 12F3)

ea

cytoskeleton

AUB01

Ubiquitin Antibody Mouse Monoclonal

ea

cytoskeleton

AUB01-HRP

Ubiquitin-HRP Antibody Mouse Monoclonal

ea

cytoskeleton

PHDG1

Acti-stain 488 phalloidin

ea

cytoskeleton

PHDH1

Acti-stain 555 phalloidin

ea

cytoskeleton

PHDN1

Acti-stain 670 phalloidin

ea

cytoskeleton

BK001

Actin Binding Protein Spin-Down Assay Biochem Kit: rabbit skeletal muscle actin

ea

cytoskeleton

BK003

Actin Polymerization Biochem Kit (fluorescence format): rabbit skeletal muscle actin

ea

cytoskeleton

AKL95

Actin protein (>95% pure): rabbit skeletal muscle

ea

cytoskeleton

AD99

Actin protein (>99% pure): bovine cardiac muscle

ea

cytoskeleton

AS99

Actin protein (>99% pure): chicken gizzard muscle

ea

cytoskeleton

AKL99

Actin protein (>99% pure): rabbit skeletal muscle

ea

cytoskeleton

AKF99

Actin protein (pre-formed filaments): rabbit skeletal muscle

ea

cytoskeleton

AP05

Actin protein (pyrene labeled): rabbit skeletal muscle

ea

cytoskeleton

APHR

Actin protein (rhodamine): human platelet

ea

cytoskeleton

AR05

Actin protein (rhodamine): rabbit skeletal muscle

ea

cytoskeleton

AT01

Alpha-actinin protein: rabbit skeletal muscle

ea

cytoskeleton

RP01P

Arp2/3 protein complex: porcine brain

ea

cytoskeleton

CS-TFC01

Cardiac Thin Filament Complex (CTFC)

ea

cytoskeleton

CF01

Cofilin 1 protein: human recombinant

ea

cytoskeleton

HPG6

Gelsolin protein: Homo sapiens recombinant

ea

cytoskeleton

MH01

Heavy meromyosin protein

ea

cytoskeleton

CS-MH03

Heavy Meromyosin Protein (HMM Fragment)

ea

cytoskeleton

CS-MYS03

Myosin – cardiac S1 fragment

ea

cytoskeleton

CS-MYS04

Myosin – skeletal muscle S1 fragment

ea

cytoskeleton

CS-MYS05

Myosin – smooth muscle S1 fragment

ea

cytoskeleton

MY02

Myosin II protein: rabbit skeletal muscle

ea

cytoskeleton

MY03

Myosin protein: bovine cardiac muscle

ea

cytoskeleton

PR02

Profilin 1 protein: Untagged, human recombinant

ea

cytoskeleton

PHDR1

Rhodamine Phalloidin

ea

cytoskeleton

CY-SC001

SiR-Actin Kit

ea

cytoskeleton

CY-SC013

SiR700-Actin Kit

ea

cytoskeleton

VCG03

WASP protein VCA domain: GST tagged: human

ea

cytoskeleton

 

 

 

上海金畔生物科技有限公司是实验试剂一站式采购服务商

1:强大的进口辐射能力,血清、抗体、耗材、大部分限制进口品等。

2:产品种类齐全,经营超过700多品牌,基本涵盖所有生物实验试剂耗材。

3:提供加急服务,货品一般1-2周到货。

4:富有竞争力的价格优势,绝大部分价格有优势。

5:多年积累良好的信誉,大部分客户提供货到付款服务。客户包括清华、北大、交大、复旦、中山等100多所高校,ROCHE,阿斯利康、国药、fisher等知药企。

6:我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech;sysy,TriLink BioTechnologies,Inc;worthington-biochem,zyagen等几十家国外公司代理。

7:我们还是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知*批发,欢迎合作。