上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
dam–/dcm– Competent E. coli
Methyltransferase deficient E. coli cells suitable for growth of plasmids free of Dam and Dcm methylation
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Dam /Dcm Competent E. Coli |NEB酶试剂 New England Biolabs
Dam Methyltransferase |NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
dam Methyltransferase
- Modifies the adenine residue (N6) of the sequence GATC
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
Lambda DNA (dam-) | NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
Lambda DNA (dam-)
- Lambda DNA is a commonly used DNA substrate
- Isolated from a dam- E.coli strain, this DNA can be digested with restriction endonucleases that are sensitive to dam methylation
- View sequence details
产品信息
来源
The phage is isolated from a heat-inducible E. coli(dam–) lambda lysogen. The DNA is isolated from purified phage by phenol extraction and dialyzed against 10 mM Tris-HCl (pH 8.0)and 1 mM EDTA.
- 产品类别:
- DNA Plasmids & Substrates Products
- 产品组分信息
产品组分信息
本产品提供以下试剂或组分：
NEB # 名称 组分货号 储存温度 数量 浓度 -
N3013S -20 Lambda DNA (dam-) N3013SVIAL -20 1 x 0.5 ml 500 µg/ml
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N3013L -20 Lambda DNA (dam-) N3013LVIAL -20 2 x 1.25 ml 500 µg/ml
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- 特性和用法
贮存溶液
10 mM Tris-HCl
1 mM EDTA
pH 8 @ 25°C - 相关产品
相关产品
- Lambda DNA
- Monarch® Plasmid Miniprep Kit
- Monarch® DNA Gel Extraction Kit
- Monarch® PCR & DNA Cleanup Kit (5 μg)
- 注意事项
- The advantage of this DNA is that it gives complete digests with those restriction endonucleases (BclI, MboI, DpnII, etc.) whose recognition sites are partially modified by most E. coli strains.
- 参考文献
- Daniels, D.L. et al. (1983). Appendix II: Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R.A. Weisberg(Ed.), Lambda-II. 519-676. New York: Cold Spring Harbor Laboratory Press.
FAQs & 问题解决指南
- FAQs
- Is the Lambda DNA (N3011) methylated?
- What is the DNA sequence of this product?
引用 & 技术文献
- 引用文献
产品引用文献查找工具
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质控、安全 & 法规
- 质控分析
每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。
- 产品说明与变更通知
产品说明与变更通知
产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]
- N3013S_L_v1
- CoA
CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]
- N3013S_L_v1_0481401
- N3013S_L_v1_0481407
- N3013S_L_v1_0481412
- N3013S_L_v1_0491412
- N3013S_L_v1_0491505
- N3013S_L_v1_0491511
- N3013S_L_v1_0491603
- N3013S_L_v1_0491608
- N3013S_L_v1_0491612
- N3013S_L_v1_0491704
- N3013S_L_v1_0491705
- N3013S_L_v1_0491707
- N3013S_L_v1_0501802
- N3013S_L_v1_0501804
- N3013L_v1_10007983
- N3013L_v1_10019245
- N3013S_v1_10019247
- N3013L_v1_10032407
- N3013L_v1_10035202
- N3013S_v1_10032410
- N3013L_v1_10055063
- N3013S_v1_10058088
- N3013L_v1_10067002
- N3013S_v1_10067003
- N3013L_v2_10071277
- N3013S_v2_10089320
- N3013L_v2_10114564
- N3013L_v2_10115054
- N3013S_v2_10119138
- N3013L_v2_10139395
- N3013S_v2_10140439
- N3013L_v2_10140441
- N3013S_v2_10157758
- SDS
以下 SDS 文件可以帮助您安全地使用该产品
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Lambda DNA (dam-)
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